Common and unique transcription factor requirements of human U1 and U6 snRNA genes
13 pages, 7 figures.-- PMID: 8253082 [PubMed].-- PMCID: PMC413633.
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| Format: | artículo biblioteca |
| Language: | English |
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National Institutes of Health (U.S.). PubMed Central
1993-09
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| Subjects: | Human Ul transcription, PSE-binding protein (PBP), RNA polymerase, Small nuclear (sn) RNA genes, TATA-binding protein (TBP), |
| Online Access: | http://hdl.handle.net/10261/26607 |
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dig-idaea-es-10261-266072019-03-20T11:25:54Z Common and unique transcription factor requirements of human U1 and U6 snRNA genes Bernués, Jordi Simmen, Kenneth A. Lewis, Joe D. Gunderson, Samuel I. Human Ul transcription PSE-binding protein (PBP) RNA polymerase Small nuclear (sn) RNA genes TATA-binding protein (TBP) 13 pages, 7 figures.-- PMID: 8253082 [PubMed].-- PMCID: PMC413633. The human U1 and U6 genes have similar basal promoter structures. A first analysis of the factor requirements for the transcription of a human U1 gene by RNA polymerase II in vitro has been undertaken, and these requirements compared with those of human U6 gene transcription by RNA polymerase III in the same extracts. Fractions containing PSE-binding protein (PBP) are shown to be essential for transcription of both genes, and further evidence that PBP itself is required for U1 as well as U6 transcription is presented. On the other hand, the two genes have distinct requirements for TATA-binding protein (TBP). On the basis of chromatographic and functional properties, the TBP, or TBP complex, required for U1 transcription appears to differ from previously described complexes required for RNA polymerase I, II or III transcription. The different TBP requirements of the U1 and U6 promoters are reflected by specific association with either TFIIB or TFIIIB respectively, thus providing a basis for differential RNA polymerase selection. We wish to thank Henk Stunnenberg, Dirk Bohmann, Francis Stewart and Peter Becker for comments on the manuscript, Jacky Schmidt and H.Stunnenberg for helpful suggestions and plasmids, the EMBL photolab, Susie Weston and Sam O'Loughlin for efficient oligonucleotide synthesis, and Maryka Kimmins and Fiona Berrie for typing the manuscript. We are particularly grateful to all other members of the group for constructive criticism and suggestions. J.B. was supported by an EEC fellowship, J.D.L. by a Human Frontiers Science Program fellowship and S.I.G. by an Alexander von Humboldt fellowship. Peer reviewed 2010-07-27T08:39:56Z 2010-07-27T08:39:56Z 1993-09 artículo http://purl.org/coar/resource_type/c_6501 EMBO Journal 12(9): 3573–3585 (1993) 0261-4189 http://hdl.handle.net/10261/26607 1460-2075 en http://www.ncbi.nlm.nih.gov/pmc/articles/PMC413633 open 3153657 bytes application/pdf National Institutes of Health (U.S.). PubMed Central |
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Human Ul transcription PSE-binding protein (PBP) RNA polymerase Small nuclear (sn) RNA genes TATA-binding protein (TBP) Human Ul transcription PSE-binding protein (PBP) RNA polymerase Small nuclear (sn) RNA genes TATA-binding protein (TBP) |
| spellingShingle |
Human Ul transcription PSE-binding protein (PBP) RNA polymerase Small nuclear (sn) RNA genes TATA-binding protein (TBP) Human Ul transcription PSE-binding protein (PBP) RNA polymerase Small nuclear (sn) RNA genes TATA-binding protein (TBP) Bernués, Jordi Simmen, Kenneth A. Lewis, Joe D. Gunderson, Samuel I. Common and unique transcription factor requirements of human U1 and U6 snRNA genes |
| description |
13 pages, 7 figures.-- PMID: 8253082 [PubMed].-- PMCID: PMC413633. |
| format |
artículo |
| topic_facet |
Human Ul transcription PSE-binding protein (PBP) RNA polymerase Small nuclear (sn) RNA genes TATA-binding protein (TBP) |
| author |
Bernués, Jordi Simmen, Kenneth A. Lewis, Joe D. Gunderson, Samuel I. |
| author_facet |
Bernués, Jordi Simmen, Kenneth A. Lewis, Joe D. Gunderson, Samuel I. |
| author_sort |
Bernués, Jordi |
| title |
Common and unique transcription factor requirements of human U1 and U6 snRNA genes |
| title_short |
Common and unique transcription factor requirements of human U1 and U6 snRNA genes |
| title_full |
Common and unique transcription factor requirements of human U1 and U6 snRNA genes |
| title_fullStr |
Common and unique transcription factor requirements of human U1 and U6 snRNA genes |
| title_full_unstemmed |
Common and unique transcription factor requirements of human U1 and U6 snRNA genes |
| title_sort |
common and unique transcription factor requirements of human u1 and u6 snrna genes |
| publisher |
National Institutes of Health (U.S.). PubMed Central |
| publishDate |
1993-09 |
| url |
http://hdl.handle.net/10261/26607 |
| work_keys_str_mv |
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