Community-level analysis of psbA gene sequences and irgarol tolerance in marine periphyton
10 pages, 4 figures, 2 tables.-- PMID: 19088321 [PubMed].-- PMCID: PMC2643554.
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American Society for Microbiology
2009-02
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dig-idaea-es-10261-259372021-12-28T16:11:00Z Community-level analysis of psbA gene sequences and irgarol tolerance in marine periphyton Eriksson, K. M. Clarke, A. K. Franzen, L. G. Kuylenstierna, M. Martínez Guijarro, Karell Blanck, H. 10 pages, 4 figures, 2 tables.-- PMID: 19088321 [PubMed].-- PMCID: PMC2643554. This study analyzes psbA gene sequences, predicted D1 protein sequences, species relative abundance, and pollution-induced community tolerance in marine periphyton communities exposed to the antifouling compound Irgarol 1051. The mechanism of action of Irgarol is the inhibition of photosynthetic electron transport at photosystem II by binding to the D1 protein. The metagenome of the communities was used to produce clone libraries containing fragments of the psbA gene encoding the D1 protein. Community tolerance was quantified with a short-term test for the inhibition of photosynthesis. The communities were established in a continuous flow of natural seawater through microcosms with or without added Irgarol. The selection pressure from Irgarol resulted in an altered species composition and an inducted community tolerance to Irgarol. Moreover, there was a very high diversity in the psbA gene sequences in the periphyton, and the composition of psbA and D1 fragments within the communities was dramatically altered by increased Irgarol exposure. Even though tolerance to this type of compound in land plants often depends on a single amino acid substitution (Ser264→Gly) in the D1 protein, this was not the case for marine periphyton species. Instead, the tolerance mechanism likely involves increased degradation of D1. When we compared sequences from low and high Irgarol exposure, differences in nonconserved amino acids were found only in the so-called PEST region of D1, which is involved in regulating its degradation. Our results suggest that environmental contamination with Irgarol has led to selection for high-turnover D1 proteins in marine periphyton communities at the west coast of Sweden. This work was supported by the European Commission (ACE project MAS3-CT98-0178), the Swedish Research Council Formas (project number 2004-2139), the University of Gothenburg Marine Research Centre, and the Memorial Foundation of Birgit and Birger Wåhlström, which are gratefully acknowledged. Peer reviewed 2010-07-02T13:20:47Z 2010-07-02T13:20:47Z 2009-02 artículo http://purl.org/coar/resource_type/c_6501 Applied and Environmental Microbiology 75(4): 897–906 (2009) 0099-2240 http://hdl.handle.net/10261/25937 10.1128/AEM.01830-08 1098-5336 19088321 en http://dx.doi.org/10.1128/AEM.01830-08 open 530392 bytes application/pdf American Society for Microbiology |
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10 pages, 4 figures, 2 tables.-- PMID: 19088321 [PubMed].-- PMCID: PMC2643554. |
| format |
artículo |
| author |
Eriksson, K. M. Clarke, A. K. Franzen, L. G. Kuylenstierna, M. Martínez Guijarro, Karell Blanck, H. |
| spellingShingle |
Eriksson, K. M. Clarke, A. K. Franzen, L. G. Kuylenstierna, M. Martínez Guijarro, Karell Blanck, H. Community-level analysis of psbA gene sequences and irgarol tolerance in marine periphyton |
| author_facet |
Eriksson, K. M. Clarke, A. K. Franzen, L. G. Kuylenstierna, M. Martínez Guijarro, Karell Blanck, H. |
| author_sort |
Eriksson, K. M. |
| title |
Community-level analysis of psbA gene sequences and irgarol tolerance in marine periphyton |
| title_short |
Community-level analysis of psbA gene sequences and irgarol tolerance in marine periphyton |
| title_full |
Community-level analysis of psbA gene sequences and irgarol tolerance in marine periphyton |
| title_fullStr |
Community-level analysis of psbA gene sequences and irgarol tolerance in marine periphyton |
| title_full_unstemmed |
Community-level analysis of psbA gene sequences and irgarol tolerance in marine periphyton |
| title_sort |
community-level analysis of psba gene sequences and irgarol tolerance in marine periphyton |
| publisher |
American Society for Microbiology |
| publishDate |
2009-02 |
| url |
http://hdl.handle.net/10261/25937 |
| work_keys_str_mv |
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