Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment
Diclofenac (DCF) belongs to the class of nonsteroidal anti-inflammatory drugs, which is one of the most consumed by population and detected in raw sewage. Several studies have reported variable removal rates by biodegradation of diclofenac in wastewater treatment plants (WWTPs). This study deals with the evaluation of the biodegradation of DCF by a bacterial consortium (obtained from pure cultures of Enterobacter hormaechei D15 and Enterobacter cloacea D16), which were isolated from household compost and Algerian WWTP, respectively, as sole carbon source and by co-metabolism, using glucose as carbon source. A 98% removal rate of DCF was observed when it is used as the sole carbon source, whilst only 44% of DCF was removed in co-metabolic conditions. Two metabolites were identified using ultra-high-performance liquid chromatography coupled to electrospray injection tandem mass spectrometry analysis (UHPLC-ESI-MS/MS); one of them was identified as 4′-hydroxy-DCF, and the second metabolite was suspected to be a nitro derivative of DCF, according to comparison with the literature. Biodegradation of DCF by this bacterial consortium generates relatively safe final by-products.
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2021-03-25
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Subjects: | Bacterial consortium, Metabolites, Biodegradation, LC-HRMS, Diclofenac, |
Online Access: | http://hdl.handle.net/10261/240483 |
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dig-idaea-es-10261-2404832022-03-25T05:30:24Z Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment Aissaoui, Salima Fagnani, Enelton Pérez, Sandra Ouled-Haddar, Houria Sifour, Mohamed Bacterial consortium Metabolites Biodegradation LC-HRMS Diclofenac Diclofenac (DCF) belongs to the class of nonsteroidal anti-inflammatory drugs, which is one of the most consumed by population and detected in raw sewage. Several studies have reported variable removal rates by biodegradation of diclofenac in wastewater treatment plants (WWTPs). This study deals with the evaluation of the biodegradation of DCF by a bacterial consortium (obtained from pure cultures of Enterobacter hormaechei D15 and Enterobacter cloacea D16), which were isolated from household compost and Algerian WWTP, respectively, as sole carbon source and by co-metabolism, using glucose as carbon source. A 98% removal rate of DCF was observed when it is used as the sole carbon source, whilst only 44% of DCF was removed in co-metabolic conditions. Two metabolites were identified using ultra-high-performance liquid chromatography coupled to electrospray injection tandem mass spectrometry analysis (UHPLC-ESI-MS/MS); one of them was identified as 4′-hydroxy-DCF, and the second metabolite was suspected to be a nitro derivative of DCF, according to comparison with the literature. Biodegradation of DCF by this bacterial consortium generates relatively safe final by-products. Authors are grateful to the Environmental Assessment and Water Research Institute of Barcelona, Department of Environmental Chemistry, Spain. E. Fagnani thanks to São Paulo Research Foundation (FAPESP), grant#2018/09875-3. S. Aissaoui is grateful to the Algerian Ministry of Higher Education and Scientific Research and DGRSDT for financial support. Peer reviewed 2021-05-11T13:40:58Z 2021-05-11T13:40:58Z 2021-03-25 artículo http://purl.org/coar/resource_type/c_6501 Brazilian Journal of Microbiology 5: 749–759 (2021) http://hdl.handle.net/10261/240483 10.1007/s42770-021-00464-9 33765286 en Postprint https://doi.org/10.1007/s42770-021-00464-9 Sí open Springer |
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Bacterial consortium Metabolites Biodegradation LC-HRMS Diclofenac Bacterial consortium Metabolites Biodegradation LC-HRMS Diclofenac |
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Bacterial consortium Metabolites Biodegradation LC-HRMS Diclofenac Bacterial consortium Metabolites Biodegradation LC-HRMS Diclofenac Aissaoui, Salima Fagnani, Enelton Pérez, Sandra Ouled-Haddar, Houria Sifour, Mohamed Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment |
description |
Diclofenac (DCF) belongs to the class of nonsteroidal anti-inflammatory drugs, which is one of the most consumed by population and detected in raw sewage. Several studies have reported variable removal rates by biodegradation of diclofenac in wastewater treatment plants (WWTPs). This study deals with the evaluation of the biodegradation of DCF by a bacterial consortium (obtained from pure cultures of Enterobacter hormaechei D15 and Enterobacter cloacea D16), which were isolated from household compost and Algerian WWTP, respectively, as sole carbon source and by co-metabolism, using glucose as carbon source. A 98% removal rate of DCF was observed when it is used as the sole carbon source, whilst only 44% of DCF was removed in co-metabolic conditions. Two metabolites were identified using ultra-high-performance liquid chromatography coupled to electrospray injection tandem mass spectrometry analysis (UHPLC-ESI-MS/MS); one of them was identified as 4′-hydroxy-DCF, and the second metabolite was suspected to be a nitro derivative of DCF, according to comparison with the literature. Biodegradation of DCF by this bacterial consortium generates relatively safe final by-products. |
format |
artículo |
topic_facet |
Bacterial consortium Metabolites Biodegradation LC-HRMS Diclofenac |
author |
Aissaoui, Salima Fagnani, Enelton Pérez, Sandra Ouled-Haddar, Houria Sifour, Mohamed |
author_facet |
Aissaoui, Salima Fagnani, Enelton Pérez, Sandra Ouled-Haddar, Houria Sifour, Mohamed |
author_sort |
Aissaoui, Salima |
title |
Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment |
title_short |
Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment |
title_full |
Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment |
title_fullStr |
Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment |
title_full_unstemmed |
Removal of diclofenac by a local bacterial consortium: UHPLC-ESI-MS/MS analysis of metabolites and ecotoxicity assessment |
title_sort |
removal of diclofenac by a local bacterial consortium: uhplc-esi-ms/ms analysis of metabolites and ecotoxicity assessment |
publisher |
Springer |
publishDate |
2021-03-25 |
url |
http://hdl.handle.net/10261/240483 |
work_keys_str_mv |
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