Photostability study of multicomponent drug formulations via MCR-ALS: The case of the hydrochlorothiazide-amiloride mixture
The kinetics and photodegradation mechanism of the pharmaceutical mixture of hydrochlorothiazide (HCT) and amiloride (AML) has been studied in depth using a chemometric approach. Water solutions of HCT and AML, separately or in binary mixtures, were irradiated with forced light at different pH values (3, 7, 9 and 12). Multivariate Curve Resolution – Alternating Least Squares (MCR-ALS) modelling has been applied to the experimental data recorded by UV spectrophotometry and HPLC-UV/MS. 78 data sets were collected and their chemometric processing has allowed the simultaneous determination of the behaviour of the two drugs in the mixture when exposed to light and the dependence of their photodegradation kinetics on pH. MCR-ALS has been applied using three different implementations. Soft-MCR-ALS and hybrid Hard/Soft-MCR-ALS have been used to resolve the experimental data and to get the equilibrium and kinetic parameters of the investigated chemical processes. A third implementation of the MCR-ALS method has been used in the analysis of the incomplete data sets obtained when UV spectrophotometric and HPLC-UV/MS data were simultaneously analysed, using a row- and column-wise incomplete augmented data matrix arrangement. In these matrices, information from HPLC-UV detector was used as a bridge between the data recorded by UV spectrophotometry (acid-base and kinetic reactions monitoring) and the data obtained by HPLC-MS.
Main Author: | |
---|---|
Format: | artículo biblioteca |
Published: |
Elsevier
2020
|
Subjects: | Amiloride, Hydrochlorothiazide, Multivariate curve resolution, UV spectrophotometry, Photostability, |
Online Access: | http://hdl.handle.net/10261/228530 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-idaea-es-10261-228530 |
---|---|
record_format |
koha |
spelling |
dig-idaea-es-10261-2285302022-07-15T04:30:38Z Photostability study of multicomponent drug formulations via MCR-ALS: The case of the hydrochlorothiazide-amiloride mixture HPLC Tauler, Romà Amiloride Hydrochlorothiazide Multivariate curve resolution UV spectrophotometry Photostability The kinetics and photodegradation mechanism of the pharmaceutical mixture of hydrochlorothiazide (HCT) and amiloride (AML) has been studied in depth using a chemometric approach. Water solutions of HCT and AML, separately or in binary mixtures, were irradiated with forced light at different pH values (3, 7, 9 and 12). Multivariate Curve Resolution – Alternating Least Squares (MCR-ALS) modelling has been applied to the experimental data recorded by UV spectrophotometry and HPLC-UV/MS. 78 data sets were collected and their chemometric processing has allowed the simultaneous determination of the behaviour of the two drugs in the mixture when exposed to light and the dependence of their photodegradation kinetics on pH. MCR-ALS has been applied using three different implementations. Soft-MCR-ALS and hybrid Hard/Soft-MCR-ALS have been used to resolve the experimental data and to get the equilibrium and kinetic parameters of the investigated chemical processes. A third implementation of the MCR-ALS method has been used in the analysis of the incomplete data sets obtained when UV spectrophotometric and HPLC-UV/MS data were simultaneously analysed, using a row- and column-wise incomplete augmented data matrix arrangement. In these matrices, information from HPLC-UV detector was used as a bridge between the data recorded by UV spectrophotometry (acid-base and kinetic reactions monitoring) and the data obtained by HPLC-MS. This study was supported by Ministero dell’Istruzione, Università e Ricerca (MIUR), Italy. 2021-02-04T09:20:04Z 2021-02-04T09:20:04Z 2020 2021-02-04T09:20:05Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.jpba.2020.113332 issn: 1873-264X Journal of Pharmaceutical and Biomedical Analysis 186 (2020) http://hdl.handle.net/10261/228530 10.1016/j.jpba.2020.113332 Postprint http://dx.doi.org/10.1016/j.jpba.2020.113332 Sí open Elsevier |
institution |
IDAEA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-idaea-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IDAEA España |
topic |
Amiloride Hydrochlorothiazide Multivariate curve resolution UV spectrophotometry Photostability Amiloride Hydrochlorothiazide Multivariate curve resolution UV spectrophotometry Photostability |
spellingShingle |
Amiloride Hydrochlorothiazide Multivariate curve resolution UV spectrophotometry Photostability Amiloride Hydrochlorothiazide Multivariate curve resolution UV spectrophotometry Photostability Tauler, Romà Photostability study of multicomponent drug formulations via MCR-ALS: The case of the hydrochlorothiazide-amiloride mixture |
description |
The kinetics and photodegradation mechanism of the pharmaceutical mixture of hydrochlorothiazide (HCT) and amiloride (AML) has been studied in depth using a chemometric approach. Water solutions of HCT and AML, separately or in binary mixtures, were irradiated with forced light at different pH values (3, 7, 9 and 12). Multivariate Curve Resolution – Alternating Least Squares (MCR-ALS) modelling has been applied to the experimental data recorded by UV spectrophotometry and HPLC-UV/MS. 78 data sets were collected and their chemometric processing has allowed the simultaneous determination of the behaviour of the two drugs in the mixture when exposed to light and the dependence of their photodegradation kinetics on pH. MCR-ALS has been applied using three different implementations. Soft-MCR-ALS and hybrid Hard/Soft-MCR-ALS have been used to resolve the experimental data and to get the equilibrium and kinetic parameters of the investigated chemical processes. A third implementation of the MCR-ALS method has been used in the analysis of the incomplete data sets obtained when UV spectrophotometric and HPLC-UV/MS data were simultaneously analysed, using a row- and column-wise incomplete augmented data matrix arrangement. In these matrices, information from HPLC-UV detector was used as a bridge between the data recorded by UV spectrophotometry (acid-base and kinetic reactions monitoring) and the data obtained by HPLC-MS. |
format |
artículo |
topic_facet |
Amiloride Hydrochlorothiazide Multivariate curve resolution UV spectrophotometry Photostability |
author |
Tauler, Romà |
author_facet |
Tauler, Romà |
author_sort |
Tauler, Romà |
title |
Photostability study of multicomponent drug formulations via MCR-ALS: The case of the hydrochlorothiazide-amiloride mixture |
title_short |
Photostability study of multicomponent drug formulations via MCR-ALS: The case of the hydrochlorothiazide-amiloride mixture |
title_full |
Photostability study of multicomponent drug formulations via MCR-ALS: The case of the hydrochlorothiazide-amiloride mixture |
title_fullStr |
Photostability study of multicomponent drug formulations via MCR-ALS: The case of the hydrochlorothiazide-amiloride mixture |
title_full_unstemmed |
Photostability study of multicomponent drug formulations via MCR-ALS: The case of the hydrochlorothiazide-amiloride mixture |
title_sort |
photostability study of multicomponent drug formulations via mcr-als: the case of the hydrochlorothiazide-amiloride mixture |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/228530 |
work_keys_str_mv |
AT taulerroma photostabilitystudyofmulticomponentdrugformulationsviamcralsthecaseofthehydrochlorothiazideamiloridemixture AT taulerroma hplc |
_version_ |
1777669492018511872 |