Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations
Vegetable crops irrigated with treated wastewater can take up the environmentally persistent pharmaceuticals diclofenac and lamotrigine. This study aimed at quantifying the uptake and translocation of the two pharmaceuticals in lettuce (Lactuca sativa) as well as on the elucidation of the molecular and physiological changes triggered by them. Therefore, plants were cultivated in a phytochamber in hydroponic systems under controlled conditions and treated independently with diclofenac (20 μg L−1) and lamotrigine (60 μg L−1) for 48 h. A low translocation of lamotrigine but not of diclofenac or its metabolite 4’-hydroxydiclofenac to leaves was observed, which corresponded with the expression of stress related genes only in roots of diclofenac treated plants. We observed an oxidative burst in roots and leaves occurring around the same time point when lamotrigine was detected in leaves. This could be responsible for the significantly changed gene expression pattern in both tissues. Our results showed for the first time that pharmaceuticals like lamotrigine or diclofenac might act as signals or zeitgebers, affecting the circadian expression of stress related genes in lettuce possibly causing a repressed physiological status of the plant.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2021-02-05
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Subjects: | Treated wastewater, Accumulation in plants, Lactuca sativa, Stress gene expression, Diurnal rhythm, |
Online Access: | http://hdl.handle.net/10261/223079 |
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dig-idaea-es-10261-2230792023-02-05T05:30:24Z Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations Bigott, Yvonne Chowdhury, Soumitra Paul Pérez, Sandra Montemurro, Nicola Manasfi, Rayana Schröder, Peter Montemurro, Nicola [0000-0002-7496-203X] Treated wastewater Accumulation in plants Lactuca sativa Stress gene expression Diurnal rhythm Vegetable crops irrigated with treated wastewater can take up the environmentally persistent pharmaceuticals diclofenac and lamotrigine. This study aimed at quantifying the uptake and translocation of the two pharmaceuticals in lettuce (Lactuca sativa) as well as on the elucidation of the molecular and physiological changes triggered by them. Therefore, plants were cultivated in a phytochamber in hydroponic systems under controlled conditions and treated independently with diclofenac (20 μg L−1) and lamotrigine (60 μg L−1) for 48 h. A low translocation of lamotrigine but not of diclofenac or its metabolite 4’-hydroxydiclofenac to leaves was observed, which corresponded with the expression of stress related genes only in roots of diclofenac treated plants. We observed an oxidative burst in roots and leaves occurring around the same time point when lamotrigine was detected in leaves. This could be responsible for the significantly changed gene expression pattern in both tissues. Our results showed for the first time that pharmaceuticals like lamotrigine or diclofenac might act as signals or zeitgebers, affecting the circadian expression of stress related genes in lettuce possibly causing a repressed physiological status of the plant. The authors would like to kindly thank Dr. Catarina Cruzeiro for her contribution to the analytical methodology and description for the liquid media samples and the detailed proofreading of this paper. Furthermore, the authors want to thank Dr. Antonios Michas for his statistical advice. Peer reviewed 2020-11-18T09:00:26Z 2020-11-18T09:00:26Z 2021-02-05 artículo http://purl.org/coar/resource_type/c_6501 Journal of Hazardous Materials 403: 123881 (2021) http://hdl.handle.net/10261/223079 10.1016/j.jhazmat.2020.123881 en #PLACEHOLDER_PARENT_METADATA_VALUE# https://doi.org/10.1016/j.jhazmat.2020.123881 Postprint Sí open Elsevier |
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Treated wastewater Accumulation in plants Lactuca sativa Stress gene expression Diurnal rhythm Treated wastewater Accumulation in plants Lactuca sativa Stress gene expression Diurnal rhythm |
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Treated wastewater Accumulation in plants Lactuca sativa Stress gene expression Diurnal rhythm Treated wastewater Accumulation in plants Lactuca sativa Stress gene expression Diurnal rhythm Bigott, Yvonne Chowdhury, Soumitra Paul Pérez, Sandra Montemurro, Nicola Manasfi, Rayana Schröder, Peter Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations |
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Vegetable crops irrigated with treated wastewater can take up the environmentally persistent pharmaceuticals diclofenac and lamotrigine. This study aimed at quantifying the uptake and translocation of the two pharmaceuticals in lettuce (Lactuca sativa) as well as on the elucidation of the molecular and physiological changes triggered by them. Therefore, plants were cultivated in a phytochamber in hydroponic systems under controlled conditions and treated independently with diclofenac (20 μg L−1) and lamotrigine (60 μg L−1) for 48 h. A low translocation of lamotrigine but not of diclofenac or its metabolite 4’-hydroxydiclofenac to leaves was observed, which corresponded with the expression of stress related genes only in roots of diclofenac treated plants. We observed an oxidative burst in roots and leaves occurring around the same time point when lamotrigine was detected in leaves. This could be responsible for the significantly changed gene expression pattern in both tissues. Our results showed for the first time that pharmaceuticals like lamotrigine or diclofenac might act as signals or zeitgebers, affecting the circadian expression of stress related genes in lettuce possibly causing a repressed physiological status of the plant. |
author2 |
Montemurro, Nicola [0000-0002-7496-203X] |
author_facet |
Montemurro, Nicola [0000-0002-7496-203X] Bigott, Yvonne Chowdhury, Soumitra Paul Pérez, Sandra Montemurro, Nicola Manasfi, Rayana Schröder, Peter |
format |
artículo |
topic_facet |
Treated wastewater Accumulation in plants Lactuca sativa Stress gene expression Diurnal rhythm |
author |
Bigott, Yvonne Chowdhury, Soumitra Paul Pérez, Sandra Montemurro, Nicola Manasfi, Rayana Schröder, Peter |
author_sort |
Bigott, Yvonne |
title |
Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations |
title_short |
Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations |
title_full |
Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations |
title_fullStr |
Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations |
title_full_unstemmed |
Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations |
title_sort |
effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (lactuca sativa) at environmentally relevant concentrations |
publisher |
Elsevier |
publishDate |
2021-02-05 |
url |
http://hdl.handle.net/10261/223079 |
work_keys_str_mv |
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