Discovery of large molecules as new biomarkers in wastewater using environmental proteomics and suitable polymer probes

The capability of monitoring large molecules as possible biomarkers in wastewater will be an important contribution to the new field of sewage epidemiology. Here, we explore the use of polymer probes together with untargeted proteomics for large scale protein analysis in sewage and treated water. Polymeric probes were immersed in the influent, anoxic reactor and effluent waters of a Spanish WWTP during 11 days. Proteins sorbed were extracted and identified by mass spectrometry. A total of 690 proteins from bacteria, plants and animals, including human, were identified showing different proteome profiles in the different sites. Bacterial proteins (510) pointed at 175 genera distributed in 22 bacteria classes. The most abundant were EF-Tu, GroEL and ATP synthase which were contributed by a high number of species. Human was the species contributing the greatest number of identified proteins (57), some in high abundance like keratins. Human proteins dominated in the influent water and were efficiently removed at the effluent. Several of the proteins identified (S100A8, uromodulin, defensins) are known disease biomarkers. This study provides the first insight into the proteome profiles present in real wastewater.

Bibliographic Details

Main Authors:	Carrascal, Montserrat, Abián, Joaquín, Ginebreda Martí, Antoni, Barceló, Damià
Other Authors:	Instituto de Salud Carlos III
Format:	artículo biblioteca
Language:	English
Published:	Elsevier BV 2020-07-25
Subjects:	Sewage epidemiology, Sewage water, HPLC-HRMS, Water fingerprinting, Proteins, Biomolecules,
Online Access:	http://hdl.handle.net/10261/217023 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100011033
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