Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes
Zebrafish models for mild, moderate, and severe acute organophosphorus poisoning were previously developed by exposing zebrafish larvae to chlopyrifos-oxon. The phenotype of these models was characterized at several levels of biological organization. Oxidative stress and mitochondrial dysfunction were found to be involved in the development of the more severe phenotype. Here we used targeted gene expression to understand the dose-responsiveness of those two pathways and their involvement on generating the different zebrafish models. As the severe phenotype is irreversible after only 3 h of exposure, we also analyzed the response of the oxidative stress pathway at 3 and 24 h. Some of the genes related to oxidative stress were already differentially expressed at 3 h. There was an increase in differentially expressed genes related to both oxidative stress and mitochondrial function from the more mild to the more severe phenotype, suggesting the involvement of these mechanisms in increasing phenotype severity. Temporal data suggest that peroxynitrite leading to lipid peroxidation might be involved in phenotype transition and irreversibility. © 2016, The Author(s).
Main Authors: | , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Springer Nature
2016-06-01
|
Subjects: | Chlorpyrifos-oxon, Gene expression, Zebrafish, Mitochondria, Oxidative stress, |
Online Access: | http://hdl.handle.net/10261/175353 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-idaea-es-10261-175353 |
---|---|
record_format |
koha |
spelling |
dig-idaea-es-10261-1753532021-12-27T16:53:14Z Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes García-Reyero, Natàlia Escalon, Lynn B. Prats, Eva Faria, Melissa Soares, Amadeu M. V. M. Raldúa, Demetrio Prats, Eva [0000-0001-7838-2027] Faria, Melissa S. [0000-0003-1451-4427] Raldúa, Demetrio [0000-0001-5256-1641] Chlorpyrifos-oxon Gene expression Zebrafish Mitochondria Oxidative stress Zebrafish models for mild, moderate, and severe acute organophosphorus poisoning were previously developed by exposing zebrafish larvae to chlopyrifos-oxon. The phenotype of these models was characterized at several levels of biological organization. Oxidative stress and mitochondrial dysfunction were found to be involved in the development of the more severe phenotype. Here we used targeted gene expression to understand the dose-responsiveness of those two pathways and their involvement on generating the different zebrafish models. As the severe phenotype is irreversible after only 3 h of exposure, we also analyzed the response of the oxidative stress pathway at 3 and 24 h. Some of the genes related to oxidative stress were already differentially expressed at 3 h. There was an increase in differentially expressed genes related to both oxidative stress and mitochondrial function from the more mild to the more severe phenotype, suggesting the involvement of these mechanisms in increasing phenotype severity. Temporal data suggest that peroxynitrite leading to lipid peroxidation might be involved in phenotype transition and irreversibility. © 2016, The Author(s). This work was supported in part by the US Army ERDC-IRO (W912HZ-13-BAA-01; D.R., N.G.R.) and Environmental Quality Research Program (N.G.R.), the NATO SfP project MD.SFPP 984777 (D.R., N.G.R.), and the National Science Foundation EPSCoR Grant EPS-0903787 (N.G.R.). Peer reviewed 2019-02-06T09:25:36Z 2019-02-06T09:25:36Z 2016-06-01 artículo http://purl.org/coar/resource_type/c_6501 Bulletin of Environmental Contamination and Toxicology 96 (6): 707-713 (2016) http://hdl.handle.net/10261/175353 10.1007/s00128-016-1798-3 27086301 en Publisher's version https://doi.org/10.1007/s00128-016-1798-3 Sí open Springer Nature |
institution |
IDAEA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-idaea-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IDAEA España |
language |
English |
topic |
Chlorpyrifos-oxon Gene expression Zebrafish Mitochondria Oxidative stress Chlorpyrifos-oxon Gene expression Zebrafish Mitochondria Oxidative stress |
spellingShingle |
Chlorpyrifos-oxon Gene expression Zebrafish Mitochondria Oxidative stress Chlorpyrifos-oxon Gene expression Zebrafish Mitochondria Oxidative stress García-Reyero, Natàlia Escalon, Lynn B. Prats, Eva Faria, Melissa Soares, Amadeu M. V. M. Raldúa, Demetrio Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes |
description |
Zebrafish models for mild, moderate, and severe acute organophosphorus poisoning were previously developed by exposing zebrafish larvae to chlopyrifos-oxon. The phenotype of these models was characterized at several levels of biological organization. Oxidative stress and mitochondrial dysfunction were found to be involved in the development of the more severe phenotype. Here we used targeted gene expression to understand the dose-responsiveness of those two pathways and their involvement on generating the different zebrafish models. As the severe phenotype is irreversible after only 3 h of exposure, we also analyzed the response of the oxidative stress pathway at 3 and 24 h. Some of the genes related to oxidative stress were already differentially expressed at 3 h. There was an increase in differentially expressed genes related to both oxidative stress and mitochondrial function from the more mild to the more severe phenotype, suggesting the involvement of these mechanisms in increasing phenotype severity. Temporal data suggest that peroxynitrite leading to lipid peroxidation might be involved in phenotype transition and irreversibility. © 2016, The Author(s). |
author2 |
Prats, Eva [0000-0001-7838-2027] |
author_facet |
Prats, Eva [0000-0001-7838-2027] García-Reyero, Natàlia Escalon, Lynn B. Prats, Eva Faria, Melissa Soares, Amadeu M. V. M. Raldúa, Demetrio |
format |
artículo |
topic_facet |
Chlorpyrifos-oxon Gene expression Zebrafish Mitochondria Oxidative stress |
author |
García-Reyero, Natàlia Escalon, Lynn B. Prats, Eva Faria, Melissa Soares, Amadeu M. V. M. Raldúa, Demetrio |
author_sort |
García-Reyero, Natàlia |
title |
Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes |
title_short |
Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes |
title_full |
Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes |
title_fullStr |
Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes |
title_full_unstemmed |
Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes |
title_sort |
targeted gene expression in zebrafish exposed to chlorpyrifos-oxon confirms phenotype-specific mechanisms leading to adverse outcomes |
publisher |
Springer Nature |
publishDate |
2016-06-01 |
url |
http://hdl.handle.net/10261/175353 |
work_keys_str_mv |
AT garciareyeronatalia targetedgeneexpressioninzebrafishexposedtochlorpyrifosoxonconfirmsphenotypespecificmechanismsleadingtoadverseoutcomes AT escalonlynnb targetedgeneexpressioninzebrafishexposedtochlorpyrifosoxonconfirmsphenotypespecificmechanismsleadingtoadverseoutcomes AT pratseva targetedgeneexpressioninzebrafishexposedtochlorpyrifosoxonconfirmsphenotypespecificmechanismsleadingtoadverseoutcomes AT fariamelissa targetedgeneexpressioninzebrafishexposedtochlorpyrifosoxonconfirmsphenotypespecificmechanismsleadingtoadverseoutcomes AT soaresamadeumvm targetedgeneexpressioninzebrafishexposedtochlorpyrifosoxonconfirmsphenotypespecificmechanismsleadingtoadverseoutcomes AT ralduademetrio targetedgeneexpressioninzebrafishexposedtochlorpyrifosoxonconfirmsphenotypespecificmechanismsleadingtoadverseoutcomes |
_version_ |
1777669317711626240 |