Analysis of bisphenol A in natural waters by means of an optical immunosensor

Analysis of bisphenol A in natural waters by means of an optical immunosensor

9 pages, 3 figures.-- PMID: 16337256 [PubMed].-- Printed version published Dec 2005.

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Bibliographic Details
Main Authors: Rodríguez-Mozaz, Sara, López de Alda, Miren, Barceló, Damià
Format: artículo biblioteca
Language:English
Published: Elsevier 2005-12-06
Subjects:Immunosensor, Bisphenol A, Water, Waterworks,
Online Access:http://hdl.handle.net/10261/15276
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spelling dig-idaea-es-10261-152762020-05-25T08:38:02Z Analysis of bisphenol A in natural waters by means of an optical immunosensor Rodríguez-Mozaz, Sara López de Alda, Miren Barceló, Damià Immunosensor Bisphenol A Water Waterworks 9 pages, 3 figures.-- PMID: 16337256 [PubMed].-- Printed version published Dec 2005. This work describes a very simple, fast and sensitive method based on the use of the optical immunosensor “RIver ANAlyser” (RIANA) to the determination of bisphenol A in a waters. RIANA is based on a rapid solid-phase indirect inhibition immunoassay that takes place at an optical transducer chip chemically modified with an analyte derivative. Fluorescence produced by labelled antibodies bound to the transducer is detected by photodiodes and can be correlated with the analyte concentration. The sensor surface can be regenerated thus allowing the performance of several measurements (around 300) with the same transducer. Each test cycle, including one regeneration step, is accomplished in 15 min. The detection limit achieved in the direct determination of bisphenol A in water with this system was 0.014 μg/L. Satisfactory repeatability, with relative standard deviations (RSD) ranging between 1.48% and 6.93% were obtained. The immunosensor method developed was applied to the monitoring of bisphenol A in various types of water collected in a waterworks (from the river water source to the finished drinking water) and validated against the results obtained in the same approach by a more traditional method, based on solid-phase extraction followed by liquid chromatography–mass spectrometry. Results obtained by both techniques were in general good agreement (considering the typical overestimation bias of immunoassays), and served to prove the satisfactory removal efficiency of the overall purification process applied in the waterworks and, in particular, of the sand filtration step. This work has been supported by the Commission of the European Community (Projects RIANA ENV4-CT95-0066, AWACSS EVK1-CT-2000-00045). Peer reviewed 2009-07-24T08:11:20Z 2009-07-24T08:11:20Z 2005-12-06 artículo http://purl.org/coar/resource_type/c_6501 Water Research 39(20): 5071-5079 (2005) 0043-1354 (Print) http://hdl.handle.net/10261/15276 10.1016/j.watres.2005.09.023 en http://dx.doi.org/10.1016/j.watres.2005.09.023 none 22195 bytes application/pdf Elsevier
institution IDAEA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-idaea-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IDAEA España
language English
topic Immunosensor
Bisphenol A
Water
Waterworks
Immunosensor
Bisphenol A
Water
Waterworks
spellingShingle Immunosensor
Bisphenol A
Water
Waterworks
Immunosensor
Bisphenol A
Water
Waterworks
Rodríguez-Mozaz, Sara
López de Alda, Miren
Barceló, Damià
Analysis of bisphenol A in natural waters by means of an optical immunosensor
description 9 pages, 3 figures.-- PMID: 16337256 [PubMed].-- Printed version published Dec 2005.
format artículo
topic_facet Immunosensor
Bisphenol A
Water
Waterworks
author Rodríguez-Mozaz, Sara
López de Alda, Miren
Barceló, Damià
author_facet Rodríguez-Mozaz, Sara
López de Alda, Miren
Barceló, Damià
author_sort Rodríguez-Mozaz, Sara
title Analysis of bisphenol A in natural waters by means of an optical immunosensor
title_short Analysis of bisphenol A in natural waters by means of an optical immunosensor
title_full Analysis of bisphenol A in natural waters by means of an optical immunosensor
title_fullStr Analysis of bisphenol A in natural waters by means of an optical immunosensor
title_full_unstemmed Analysis of bisphenol A in natural waters by means of an optical immunosensor
title_sort analysis of bisphenol a in natural waters by means of an optical immunosensor
publisher Elsevier
publishDate 2005-12-06
url http://hdl.handle.net/10261/15276
work_keys_str_mv AT rodriguezmozazsara analysisofbisphenolainnaturalwatersbymeansofanopticalimmunosensor
AT lopezdealdamiren analysisofbisphenolainnaturalwatersbymeansofanopticalimmunosensor
AT barcelodamia analysisofbisphenolainnaturalwatersbymeansofanopticalimmunosensor
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