Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass
8 pages, 4 figures, 3 tables
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Elsevier
2016-06
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| Subjects: | Progestins, Sea bass, Sex steroids, Metabolism, cyp17a1, |
| Online Access: | http://hdl.handle.net/10261/133350 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100007273 |
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dig-idaea-es-10261-1333502021-09-17T06:05:46Z Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass Blanco, María Fernandes, Denise Medina, Paula Blázquez, Mercedes Porte Visa, Cinta Fundação para a Ciência e a Tecnologia (Portugal) Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Progestins Sea bass Sex steroids Metabolism cyp17a1 8 pages, 4 figures, 3 tables Drospirenone (DRO) is one of the most widely used progestins in contraceptive treatments and hormone replacement therapies. The pharmacokinetics and potential toxicological effects of DRO were investigated in juvenile sea bass (Dicentrarchus labrax) exposed through the diet (0.01–10 μg DRO/g) for up to 31 days. DRO was detected in the blood (4–27 ng/mL) of fish exposed to the highest concentration, with no significant bioaccumulation over time and no alteration of hepatic metabolizing enzymes, namely, CYP1A and CYP3A-catalysed activities and UDP-glucuronyltransferase (UGT). Pregnenolone (P5), progesterone (P4), 17α-hydroxyprogesterone (17P4), 17α-hydroxypregnenolone (17P5), androstenedione (AD) and testosterone (T) were determined in plasma and gene expression of cyp17a1, cyp19a1a and cyp11β analysed by qRT-PCR in gonads. The significant increase in plasmatic levels of 17P5, 17P4 and AD detected after 31 days exposure to 10 ng DRO/g together with the increased expression of cyp17a1 in females evidence the ability of DRO to alter steroid synthesis at low intake concentrations (7 ng DRO/day). However, the potential consequences of this steroid shift for female reproduction remain to be investigated Maria Blanco acknowledges a pre-doctoral fellowship (FPI, BES-2012-054438) from the Spanish Government and Paula Medina the pre-doctoral fellowship MECE2, ANT0806. Denise Fernandes acknowledges a post-doctoral fellowship (SFRH/BPD/34289/2006) from the Portuguese Fundação para a Ciência e Tecnologia (FCT) of the Ministry of Science and Technology of Portugal. This study was supported by the Spanish National Plan of Research (Project Ref. CGL2011-24538) Peer Reviewed 2016-06-13T06:53:26Z 2016-06-13T06:53:26Z 2016-06 2016-06-13T06:53:26Z artículo http://purl.org/coar/resource_type/c_6501 issn: 0269-7491 e-issn: 1873-6424 Environmental Pollution 213: 541-548 (2016) http://hdl.handle.net/10261/133350 10.1016/j.envpol.2016.03.007 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100007273 https://dx.doi.org/10.1016/j.envpol.2016.03.007 Sí none Elsevier |
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Progestins Sea bass Sex steroids Metabolism cyp17a1 Progestins Sea bass Sex steroids Metabolism cyp17a1 |
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Progestins Sea bass Sex steroids Metabolism cyp17a1 Progestins Sea bass Sex steroids Metabolism cyp17a1 Blanco, María Fernandes, Denise Medina, Paula Blázquez, Mercedes Porte Visa, Cinta Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass |
| description |
8 pages, 4 figures, 3 tables |
| author2 |
Fundação para a Ciência e a Tecnologia (Portugal) |
| author_facet |
Fundação para a Ciência e a Tecnologia (Portugal) Blanco, María Fernandes, Denise Medina, Paula Blázquez, Mercedes Porte Visa, Cinta |
| format |
artículo |
| topic_facet |
Progestins Sea bass Sex steroids Metabolism cyp17a1 |
| author |
Blanco, María Fernandes, Denise Medina, Paula Blázquez, Mercedes Porte Visa, Cinta |
| author_sort |
Blanco, María |
| title |
Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass |
| title_short |
Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass |
| title_full |
Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass |
| title_fullStr |
Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass |
| title_full_unstemmed |
Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass |
| title_sort |
drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass |
| publisher |
Elsevier |
| publishDate |
2016-06 |
| url |
http://hdl.handle.net/10261/133350 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100007273 |
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