Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)

Farré, Marinella; Brix, Rikke; Kuster, Marina; Rubio, Fernando; Goda, Yasuhiro; López de Alda, Miren; Barceló, Damià

Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)

11 pages, 5 figures, 5 tables.

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Bibliographic Details
Main Authors:	Farré, Marinella, Brix, Rikke, Kuster, Marina, Rubio, Fernando, Goda, Yasuhiro, López de Alda, Miren, Barceló, Damià
Format:	artículo biblioteca
Language:	English
Published:	Springer 2006-07-01
Subjects:	Immunoassay, ELISA, Estrogens, LC–MS/MS,
Online Access:	http://hdl.handle.net/10261/11798
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spelling	dig-idaea-es-10261-117982020-05-25T08:38:02Z Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ) Farré, Marinella Brix, Rikke Kuster, Marina Rubio, Fernando Goda, Yasuhiro López de Alda, Miren Barceló, Damià Immunoassay ELISA Estrogens LC–MS/MS 11 pages, 5 figures, 5 tables. In this work four different commercially available enzyme-linked immunosorbent assays (ELISA) (from Japan EnviroChemicals, Ltd., Tokyo, Japan) were evaluated in terms of performance for the rapid screening of estrogens in different water matrices, including natural and spiked samples from urban wastewater, river water and ground water. All four test kits are based on monoclonal antibodies. The compounds detected by these immunoassays are (1) 17-β-estradiol, (2) estrone, (3) 17-α-ethynyl estradiol and (4) estrogens in general, with high recognition properties for 17-β-estradiol, estrone and estriol. Standards were prepared in water containing 10% (v/v) methanol. The IC 50 (corresponding to the 50% of the effective concentration) values, the dynamic ranges, and the limits of detection of the ELISA kits were 0.060–0.304 μg/L, 0.05–5 μg/L and 0.05 μg/L, respectively. All samples were extracted by solid-phase extraction (SPE) beforehand, and the evaluation was carried out by comparing the results obtained by ELISA with those obtained by HPLC–MS/MS using a triple quadrupole (QqQ) instrument. In addition, two different solid-phase extraction procedures were carried out and compared. Except for moderate overestimation in the results observed with the ELISA kits in the analysis of complex wastewater samples, the results obtained using all of the tested techniques were generally in very good agreement. This work was supported by the EU through the projects SWIFT-WFD (SSPI-CT-2003-502492) and MODELKEY (SSPI-CT-2003-511237-2), and by the Ministerio de Ciencia y Tecnología through the projects CTM2005-24255-E and EVITA (CTM2004-06265-C03-01/TECNO). Marinella Farré thanks the support from the Ministerio de Educación y Ciencia through the Juan de la Cierva program. Peer reviewed 2009-03-23T12:37:37Z 2009-03-23T12:37:37Z 2006-07-01 artículo http://purl.org/coar/resource_type/c_6501 Analytical and Bioanalytical Chemistry 385(6): 1001-1011 (2006) 1618-2642 http://hdl.handle.net/10261/11798 10.1007/s00216-006-0562-7 1618-2650 en http://dx.doi.org/10.1007/s00216-006-0562-7 none 19968 bytes application/msword Springer
institution	IDAEA ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-idaea-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IDAEA España
language	English
topic	Immunoassay ELISA Estrogens LC–MS/MS Immunoassay ELISA Estrogens LC–MS/MS
spellingShingle	Immunoassay ELISA Estrogens LC–MS/MS Immunoassay ELISA Estrogens LC–MS/MS Farré, Marinella Brix, Rikke Kuster, Marina Rubio, Fernando Goda, Yasuhiro López de Alda, Miren Barceló, Damià Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)
description	11 pages, 5 figures, 5 tables.
format	artículo
topic_facet	Immunoassay ELISA Estrogens LC–MS/MS
author	Farré, Marinella Brix, Rikke Kuster, Marina Rubio, Fernando Goda, Yasuhiro López de Alda, Miren Barceló, Damià
author_facet	Farré, Marinella Brix, Rikke Kuster, Marina Rubio, Fernando Goda, Yasuhiro López de Alda, Miren Barceló, Damià
author_sort	Farré, Marinella
title	Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)
title_short	Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)
title_full	Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)
title_fullStr	Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)
title_full_unstemmed	Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)
title_sort	evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry hplc–ms/ms (qqq)
publisher	Springer
publishDate	2006-07-01
url	http://hdl.handle.net/10261/11798
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Evaluation of commercial immunoassays for the detection of estrogens in water by comparison with high-performance liquid chromatography tandem mass spectrometry HPLC–MS/MS (QqQ)

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