SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua

SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua

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Trabajo presentado en el IOBC-WPRS Working Group Insect Pathogens and Insect Parasitic Nematodes, celebrado en Zagreb del 16 al 20 de junio de 2013.

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Bibliographic Details
Main Authors: Virto, Cristina, Navarro, David, Tellez, María del Mar, Murillo, Rosa, Williams, Trevor G., Caballero, Primitivo
Format: comunicación de congreso biblioteca
Published: International Organisation for Biological and Integrated Control of Noxious Animals and Plants 2013-06
Subjects:Stress factors, NPVs reactivation, Spodoptera exigua multiple nucleopolyhedrovirus,
Online Access:http://hdl.handle.net/10261/99058
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spelling dig-idab-es-10261-990582016-09-07T10:33:40Z SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua Virto, Cristina Navarro, David Tellez, María del Mar Murillo, Rosa Williams, Trevor G. Caballero, Primitivo Stress factors NPVs reactivation Spodoptera exigua multiple nucleopolyhedrovirus Trabajo presentado en el IOBC-WPRS Working Group Insect Pathogens and Insect Parasitic Nematodes, celebrado en Zagreb del 16 al 20 de junio de 2013. The aim of this study is to evaluate the effect of different stress factors on covertly infected Spodptera exigua larvae in terms of virus reactivation. For this, adult survivors that had ingested occlusion bodies of S. exigua multiple nucleopolyhedrovirus (SeMNPV) were mated and the subsequent generation (F1) tested for virus reactivation in the second instar both in laboratory and field conditions. In the laboratory a number of treatments were tested including chemical stressors, inoculation with permissive and non permissive NPV species and Bacillus thuringiensis spores and crystals. Both, parental and F1 adults were confirmed to harbor the infection by qPCR. Reactivation was observed in 0.1% copper sulfate, 1% iron sulfate, and 1 ppm sodium selenate treatment that resulted in 12, 15, and 41% mortality due to SeMNPV, while no larvae with symptoms of viral infection were registered in virus-free controls. No effect on NPV-induced mortality was detected after inoculation with heterologous virus. Lately field trials were carried out by artificial infestation of pepper crops in experimental greenhouses using sublethally infected S. exigua larvae to evaluate copper sulfate and sodium selenate as activation factors. Hardly any NPV-induced mortality was observed in those larvae treated in field conditions. This work has been funded from the Ministerio de Ciencia e Innovación (Spain) by the project number AGL2011-30352-C02-01. C.V. received a predoctoral fellowship from the Universidad Publica de Navarra. Peer Reviewed 2014-06-27T07:56:14Z 2014-06-27T07:56:14Z 2013-06 2014-06-27T07:56:14Z comunicación de congreso http://purl.org/coar/resource_type/c_5794 IOBC-WPRS Bulletin 90: 203-205 (2013) 978-92-9067-268-5 1027-3115 http://hdl.handle.net/10261/99058 open International Organisation for Biological and Integrated Control of Noxious Animals and Plants
institution IDAB ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-idab-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IDAB España
topic Stress factors
NPVs reactivation
Spodoptera exigua multiple nucleopolyhedrovirus
Stress factors
NPVs reactivation
Spodoptera exigua multiple nucleopolyhedrovirus
spellingShingle Stress factors
NPVs reactivation
Spodoptera exigua multiple nucleopolyhedrovirus
Stress factors
NPVs reactivation
Spodoptera exigua multiple nucleopolyhedrovirus
Virto, Cristina
Navarro, David
Tellez, María del Mar
Murillo, Rosa
Williams, Trevor G.
Caballero, Primitivo
SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua
description Trabajo presentado en el IOBC-WPRS Working Group Insect Pathogens and Insect Parasitic Nematodes, celebrado en Zagreb del 16 al 20 de junio de 2013.
format comunicación de congreso
topic_facet Stress factors
NPVs reactivation
Spodoptera exigua multiple nucleopolyhedrovirus
author Virto, Cristina
Navarro, David
Tellez, María del Mar
Murillo, Rosa
Williams, Trevor G.
Caballero, Primitivo
author_facet Virto, Cristina
Navarro, David
Tellez, María del Mar
Murillo, Rosa
Williams, Trevor G.
Caballero, Primitivo
author_sort Virto, Cristina
title SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua
title_short SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua
title_full SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua
title_fullStr SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua
title_full_unstemmed SeMNPV reactivation through stress factors in covertly infected Spodoptera exigua
title_sort semnpv reactivation through stress factors in covertly infected spodoptera exigua
publisher International Organisation for Biological and Integrated Control of Noxious Animals and Plants
publishDate 2013-06
url http://hdl.handle.net/10261/99058
work_keys_str_mv AT virtocristina semnpvreactivationthroughstressfactorsincovertlyinfectedspodopteraexigua
AT navarrodavid semnpvreactivationthroughstressfactorsincovertlyinfectedspodopteraexigua
AT tellezmariadelmar semnpvreactivationthroughstressfactorsincovertlyinfectedspodopteraexigua
AT murillorosa semnpvreactivationthroughstressfactorsincovertlyinfectedspodopteraexigua
AT williamstrevorg semnpvreactivationthroughstressfactorsincovertlyinfectedspodopteraexigua
AT caballeroprimitivo semnpvreactivationthroughstressfactorsincovertlyinfectedspodopteraexigua
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