Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora
The aim of this work was to isolate and characterize novel Phthorimaea operculella granulovirus (PhopGV) strains from Costa Rican soils. Three novel strains, named PhopGV-CR3, PhopGV-CR4 and PhopGV-CR5, were isolated from three locations in Costa Rica, Alvarado, Zarcero and Abangares, respectively, by means of soaking potato tubers with diluted soil samples. An additional strain, PhopGV-CR2, was identified from diseased larvae from a Tecia solanivora laboratory culture. Restriction fragment length polymorphisms obtained for each isolate with six restriction endonucleases (RENs) allowed their identification as four distinct PhopGV strains. Both REN and Polymerase chain reaction analyses indicated the existence of an array of genotypic variants present in all isolates. Bioassays in P. operculella and T. solanivora showed that PhopGV-CR3 was well adapted to the two coexisting hosts with high levels of pathogenicity against both pest species. The mean lethal dose values of this strain were 2.8OBs/mm 2 for P. operculella and less than 0.5OBs/mm 2 for T. solanivora. We conclude that PhopGV-CR3 shows great promise for soil application against these pests in Costa Rican potato crops. © 2011 Blackwell Verlag, GmbH.
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Format: | artículo biblioteca |
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Blackwell Publishing
2012
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Subjects: | Viral host adaptation, Soil isolates, Potato tuberworm, PhopGV, Persistence, Bioinsecticides, Betabaculovirus, |
Online Access: | http://hdl.handle.net/10261/97303 |
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dig-idab-es-10261-973032016-09-07T09:43:16Z Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora Gómez-Bonilla, Yannery López-Ferber, Miguel Caballero, Primitivo Léry, Xavier Muñoz, Delia Viral host adaptation Soil isolates Potato tuberworm PhopGV Persistence Bioinsecticides Betabaculovirus The aim of this work was to isolate and characterize novel Phthorimaea operculella granulovirus (PhopGV) strains from Costa Rican soils. Three novel strains, named PhopGV-CR3, PhopGV-CR4 and PhopGV-CR5, were isolated from three locations in Costa Rica, Alvarado, Zarcero and Abangares, respectively, by means of soaking potato tubers with diluted soil samples. An additional strain, PhopGV-CR2, was identified from diseased larvae from a Tecia solanivora laboratory culture. Restriction fragment length polymorphisms obtained for each isolate with six restriction endonucleases (RENs) allowed their identification as four distinct PhopGV strains. Both REN and Polymerase chain reaction analyses indicated the existence of an array of genotypic variants present in all isolates. Bioassays in P. operculella and T. solanivora showed that PhopGV-CR3 was well adapted to the two coexisting hosts with high levels of pathogenicity against both pest species. The mean lethal dose values of this strain were 2.8OBs/mm 2 for P. operculella and less than 0.5OBs/mm 2 for T. solanivora. We conclude that PhopGV-CR3 shows great promise for soil application against these pests in Costa Rican potato crops. © 2011 Blackwell Verlag, GmbH. Yannery Gomez-Bonilla was the recipient of scholarships from the Spanish National Institute of Agricultural Research (INIA), the Costa Rican Ministry of Science and Technology (MICIT- CONICIT) and INTA. We are very grateful to Trevor Williams for his help with the English and the editing of this paper as well as for his constant support. Peer Reviewed 2014-05-27T11:16:00Z 2014-05-27T11:16:00Z 2012 2014-05-27T11:16:00Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1111/j.1439-0418.2011.01679.x issn: 0931-2048 e-issn: 1439-0418 Journal of Applied Entomology 136(7): 530-538 (2012) http://hdl.handle.net/10261/97303 10.1111/j.1439-0418.2011.01679.x none Blackwell Publishing |
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Viral host adaptation Soil isolates Potato tuberworm PhopGV Persistence Bioinsecticides Betabaculovirus Viral host adaptation Soil isolates Potato tuberworm PhopGV Persistence Bioinsecticides Betabaculovirus |
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Viral host adaptation Soil isolates Potato tuberworm PhopGV Persistence Bioinsecticides Betabaculovirus Viral host adaptation Soil isolates Potato tuberworm PhopGV Persistence Bioinsecticides Betabaculovirus Gómez-Bonilla, Yannery López-Ferber, Miguel Caballero, Primitivo Léry, Xavier Muñoz, Delia Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora |
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The aim of this work was to isolate and characterize novel Phthorimaea operculella granulovirus (PhopGV) strains from Costa Rican soils. Three novel strains, named PhopGV-CR3, PhopGV-CR4 and PhopGV-CR5, were isolated from three locations in Costa Rica, Alvarado, Zarcero and Abangares, respectively, by means of soaking potato tubers with diluted soil samples. An additional strain, PhopGV-CR2, was identified from diseased larvae from a Tecia solanivora laboratory culture. Restriction fragment length polymorphisms obtained for each isolate with six restriction endonucleases (RENs) allowed their identification as four distinct PhopGV strains. Both REN and Polymerase chain reaction analyses indicated the existence of an array of genotypic variants present in all isolates. Bioassays in P. operculella and T. solanivora showed that PhopGV-CR3 was well adapted to the two coexisting hosts with high levels of pathogenicity against both pest species. The mean lethal dose values of this strain were 2.8OBs/mm 2 for P. operculella and less than 0.5OBs/mm 2 for T. solanivora. We conclude that PhopGV-CR3 shows great promise for soil application against these pests in Costa Rican potato crops. © 2011 Blackwell Verlag, GmbH. |
format |
artículo |
topic_facet |
Viral host adaptation Soil isolates Potato tuberworm PhopGV Persistence Bioinsecticides Betabaculovirus |
author |
Gómez-Bonilla, Yannery López-Ferber, Miguel Caballero, Primitivo Léry, Xavier Muñoz, Delia |
author_facet |
Gómez-Bonilla, Yannery López-Ferber, Miguel Caballero, Primitivo Léry, Xavier Muñoz, Delia |
author_sort |
Gómez-Bonilla, Yannery |
title |
Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora |
title_short |
Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora |
title_full |
Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora |
title_fullStr |
Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora |
title_full_unstemmed |
Costa Rican soils contain highly insecticidal granulovirus strains against Phthorimaea operculella and Tecia solanivora |
title_sort |
costa rican soils contain highly insecticidal granulovirus strains against phthorimaea operculella and tecia solanivora |
publisher |
Blackwell Publishing |
publishDate |
2012 |
url |
http://hdl.handle.net/10261/97303 |
work_keys_str_mv |
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