Expression of Bacillus thuringiensis Cry1Ia7 toxin in Pseudomonas fluorescens confers protection against UV radiation
Trabajo presentado en la 45th Annual Meeting of the Society for Invertebrate Pathology, celebrada en Buenos Aires del 5 al 9 de agosto de 2012.
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Society for Invertebrate Pathology
2012-08
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dig-idab-es-10261-729572016-09-07T10:42:04Z Expression of Bacillus thuringiensis Cry1Ia7 toxin in Pseudomonas fluorescens confers protection against UV radiation Ruiz de Escudero, Íñigo Asensio, Aaron C. Nepote-Górriz, Ainara Muñoz, Delia Caballero, Primitivo Trabajo presentado en la 45th Annual Meeting of the Society for Invertebrate Pathology, celebrada en Buenos Aires del 5 al 9 de agosto de 2012. The Bacillus thuringiensis Cry1Ia7 insecticidal protein is toxic against several lepidopteran species, including the European grapevine moth Lobesia botrana (Lepidoptera: Tortricidae). Cry1Ia7 does not form part of the paraesporal crystal but is expressed and secreted during the vegetative phase.This,along with its rapid degradation upon exposure to solar ultraviolet radiation hinders its use as a biological insecticide.To address this problem, cry1Ia7 was cloned into a UV-resistant P. fluorescens strain. Cry1Ia7 was expressed and encapsulated in vegetative P. fluorescens cells following established protocols. The toxicity of Cry1Ia7 protein produced in P. fluorescenswas purified inanamylose affinity column usingamaltose binding protein (Pf-MBP-Cry1Ia7) and compared with that of Cry1Ia7 expressed and encapsulated in P.fluorescens(Pf-Cry1Ia7).Both proteins were active against L. botrana,and their mean lethal concentrations (LC50 values) were not statistically different (10.63and12.6/g/ml,respectively).The activity of free Pf-MBP- Cry1Ia7 protein was reduced 10-fold following exposure to a UV source; however, the LC50 of Pf-Cry1Ia7 encapsulated in P. fluorescens was not significantly affected by exposure to UV, due to the photoprotection offered by P. fluorescens encapsulation.This system of protein production can be used as a model for other Bacillus thuringiensis proteins that would benefit from improved protection against solar UV radiation. Peer Reviewed 2013-03-26T10:30:20Z 2013-03-26T10:30:20Z 2012-08 2013-03-26T10:30:21Z comunicación de congreso http://purl.org/coar/resource_type/c_5794 45th Annual Meeting of the Society for Invertebrate Pathology (2012) 2012 International Congress on Invertebrate Pathology and Microbial Control http://hdl.handle.net/10261/72957 en none Society for Invertebrate Pathology |
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Trabajo presentado en la 45th Annual Meeting of the Society for Invertebrate Pathology, celebrada en Buenos Aires del 5 al 9 de agosto de 2012. |
| format |
comunicación de congreso |
| author |
Ruiz de Escudero, Íñigo Asensio, Aaron C. Nepote-Górriz, Ainara Muñoz, Delia Caballero, Primitivo |
| spellingShingle |
Ruiz de Escudero, Íñigo Asensio, Aaron C. Nepote-Górriz, Ainara Muñoz, Delia Caballero, Primitivo Expression of Bacillus thuringiensis Cry1Ia7 toxin in Pseudomonas fluorescens confers protection against UV radiation |
| author_facet |
Ruiz de Escudero, Íñigo Asensio, Aaron C. Nepote-Górriz, Ainara Muñoz, Delia Caballero, Primitivo |
| author_sort |
Ruiz de Escudero, Íñigo |
| title |
Expression of Bacillus thuringiensis Cry1Ia7 toxin in Pseudomonas fluorescens confers protection against UV radiation |
| title_short |
Expression of Bacillus thuringiensis Cry1Ia7 toxin in Pseudomonas fluorescens confers protection against UV radiation |
| title_full |
Expression of Bacillus thuringiensis Cry1Ia7 toxin in Pseudomonas fluorescens confers protection against UV radiation |
| title_fullStr |
Expression of Bacillus thuringiensis Cry1Ia7 toxin in Pseudomonas fluorescens confers protection against UV radiation |
| title_full_unstemmed |
Expression of Bacillus thuringiensis Cry1Ia7 toxin in Pseudomonas fluorescens confers protection against UV radiation |
| title_sort |
expression of bacillus thuringiensis cry1ia7 toxin in pseudomonas fluorescens confers protection against uv radiation |
| publisher |
Society for Invertebrate Pathology |
| publishDate |
2012-08 |
| url |
http://hdl.handle.net/10261/72957 |
| work_keys_str_mv |
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