Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva
8 p., 3 figures, 1 table of contents and bibliography
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Elsevier
2010-11
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| Online Access: | http://hdl.handle.net/10261/48483 |
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dig-idab-es-10261-484832016-09-07T10:56:00Z Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva Fernández San Millán, Alicia Gómez Sebastián, Silvia Núñez, María Carmen Veramendi, Jon Escribano, José M. 8 p., 3 figures, 1 table of contents and bibliography Sexually transmitt ed human papillomavirus (HPV) infections cause most cervical cancers. The inability to propagate HPV in vitro has led to the development of alternative approaches to antigen production for vaccine development. In the present work we describe a l ow - cost eff icient production of HPV virus - like particles (VLPs) in a non - fermentative system based on baculovirus and Trichoplusia ni ( T. ni ) insects. The L1 protein from HPV 16 was expressed by a recombinant baculovirus in larva at levels that reached 18 to 21 mg pe r g of fresh insect biomass. It represents about 2.5 times the L1 production yields obtained per g of insect cells using the same recombinant baculovirus. Under electron microscopy, purified VLPs produced in larvae were indistinguishable from those produce d in Sf - 21 insect cell cultures, presenting identical shape and size . Immunization of mice with the insect - derived VLPs induced a potent immune response similar to that obtained with insect cells - derived VLPs. The use of live insect larvae as “mini bioreac tors” opens up the possibility of cost - effective production of a vaccine against cervical cancer produced by papillomavirus, with special application in human populations where production costs restrain their use. This work was partially supported by Grants BIO2005-00155 and CPE03-022-C5-2 from the Ministerio de Educación y Ciencia and INIA, respectively, and by funds from the company Alternative Gene Expression S.L. (ALGENEX). Peer reviewed 2012-04-18T11:29:49Z 2012-04-18T11:29:49Z 2010-11 artículo http://purl.org/coar/resource_type/c_6501 Protein Expression and Purification 74(1): 1-8 (2010) 1046-5928 http://hdl.handle.net/10261/48483 10.1016/j.pep.2010.06.009 en http://dx.doi.org/10.1016/j.pep.2010.06.009 open Elsevier Academic Press |
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| language |
English |
| description |
8 p., 3 figures, 1 table of contents and bibliography |
| format |
artículo |
| author |
Fernández San Millán, Alicia Gómez Sebastián, Silvia Núñez, María Carmen Veramendi, Jon Escribano, José M. |
| spellingShingle |
Fernández San Millán, Alicia Gómez Sebastián, Silvia Núñez, María Carmen Veramendi, Jon Escribano, José M. Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva |
| author_facet |
Fernández San Millán, Alicia Gómez Sebastián, Silvia Núñez, María Carmen Veramendi, Jon Escribano, José M. |
| author_sort |
Fernández San Millán, Alicia |
| title |
Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva |
| title_short |
Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva |
| title_full |
Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva |
| title_fullStr |
Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva |
| title_full_unstemmed |
Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva |
| title_sort |
human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva |
| publisher |
Elsevier |
| publishDate |
2010-11 |
| url |
http://hdl.handle.net/10261/48483 |
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