Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research
Aluminium (Al) hydroxide use as adjuvant induces local formation of long-lasting subcutaneous granulomas in sheep. Macrophages within these granulomas have been identified as a new small ruminant lentivirus (SRLV) replication site in naturally infected animals. Diagnosis of Al hydroxide-induced granulomas in sheep is mostly based on postmortem observations but little information is available on in vivo detection. Computed tomography (CT) is used for studying these reactions in other animal species. To determine if CT could be a tool for in vivo diagnosis and research of subcutaneous Al hydroxide-induced granulomas in sheep. A retrospective survey on thoracic CT scans was performed on 46 adult sheep. Analysis included absence or presence, number and location of subcutaneous nodules. Thoracic CT scans and pathological studies were prescribed to two further sheep. Single or multiple subcutaneous nodules were detected in 26 (56.52%) sheep. One or two nodules per animal were most often observed (36.95%). Size ranged between 1.5 and 4.5 cm. Pre-contrast two-dimensional (2D) CT images showed focal or multifocal increases in subcutaneous tissue thickness. Post-contrast 2D CT images revealed hypointense areas in the centre. Histopathology indicated the presence of granulomas composed by a large number of activated macrophages, surrounding a central core of necrosis. Large intracytoplasmic Al-positive aggregates were demonstrated by lumogallion staining. CT is a useful tool to detect subcutaneous Al hydroxide-induced granulomas in vivo in sheep. CT provides a diagnostic and research tool that can be very useful in future works in Al hydroxide-induced pathology, SRLV infection, or both.
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John Wiley & Sons
2021-09
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Subjects: | Non-invasive diagnosis, Ovine, Small ruminant lentiviruses, Subcutaneous granuloma, |
Online Access: | http://hdl.handle.net/10261/261408 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100010067 |
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dig-idab-es-10261-2614082022-02-22T02:22:26Z Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research Rodríguez-Largo, Ana Castells, Enrique Miguel, Ricardo de Gómez, Álex Ruiz, Héctor Pérez, Marta M. Reina, Ramsés Ferrer, Luis M. Luján, Lluís Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Gobierno de Aragón Non-invasive diagnosis Ovine Small ruminant lentiviruses Subcutaneous granuloma Aluminium (Al) hydroxide use as adjuvant induces local formation of long-lasting subcutaneous granulomas in sheep. Macrophages within these granulomas have been identified as a new small ruminant lentivirus (SRLV) replication site in naturally infected animals. Diagnosis of Al hydroxide-induced granulomas in sheep is mostly based on postmortem observations but little information is available on in vivo detection. Computed tomography (CT) is used for studying these reactions in other animal species. To determine if CT could be a tool for in vivo diagnosis and research of subcutaneous Al hydroxide-induced granulomas in sheep. A retrospective survey on thoracic CT scans was performed on 46 adult sheep. Analysis included absence or presence, number and location of subcutaneous nodules. Thoracic CT scans and pathological studies were prescribed to two further sheep. Single or multiple subcutaneous nodules were detected in 26 (56.52%) sheep. One or two nodules per animal were most often observed (36.95%). Size ranged between 1.5 and 4.5 cm. Pre-contrast two-dimensional (2D) CT images showed focal or multifocal increases in subcutaneous tissue thickness. Post-contrast 2D CT images revealed hypointense areas in the centre. Histopathology indicated the presence of granulomas composed by a large number of activated macrophages, surrounding a central core of necrosis. Large intracytoplasmic Al-positive aggregates were demonstrated by lumogallion staining. CT is a useful tool to detect subcutaneous Al hydroxide-induced granulomas in vivo in sheep. CT provides a diagnostic and research tool that can be very useful in future works in Al hydroxide-induced pathology, SRLV infection, or both. Spanish Ministry of Science, Innovation and Universities,Grant/Award Number:RTI2018- 096172-B-C33; Recognized ResearchGroups ofGovernment of Aragón, Grant/AwardNumber: A17_17R 2022-02-21T12:50:03Z 2022-02-21T12:50:03Z 2021-09 2022-02-21T12:50:03Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1002/vms3.572 e-issn: 2053-1095 Veterinary Medicine and Science 7(5): 1757-1761 (2021) http://hdl.handle.net/10261/261408 10.1002/vms3.572 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100010067 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096172-B-C33/ES/GRANULOMAS POSTVACUNALES EN OVINO: RELACION CON LENTIVIRUS DE LOS PEQUEÑOS RUMIANTES Y ESTUDIO DE UN ADYUVANTE VACUNAL ALTERNATIVO/ Publisher's version http://dx.doi.org/10.1002/vms3.572 Sí open John Wiley & Sons |
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Non-invasive diagnosis Ovine Small ruminant lentiviruses Subcutaneous granuloma Non-invasive diagnosis Ovine Small ruminant lentiviruses Subcutaneous granuloma |
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Non-invasive diagnosis Ovine Small ruminant lentiviruses Subcutaneous granuloma Non-invasive diagnosis Ovine Small ruminant lentiviruses Subcutaneous granuloma Rodríguez-Largo, Ana Castells, Enrique Miguel, Ricardo de Gómez, Álex Ruiz, Héctor Pérez, Marta M. Reina, Ramsés Ferrer, Luis M. Luján, Lluís Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research |
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Aluminium (Al) hydroxide use as adjuvant induces local formation of long-lasting subcutaneous granulomas in sheep. Macrophages within these granulomas have been identified as a new small ruminant lentivirus (SRLV) replication site in naturally infected animals. Diagnosis of Al hydroxide-induced granulomas in sheep is mostly based on postmortem observations but little information is available on in vivo detection. Computed tomography (CT) is used for studying these reactions in other animal species. To determine if CT could be a tool for in vivo diagnosis and research of subcutaneous Al hydroxide-induced granulomas in sheep. A retrospective survey on thoracic CT scans was performed on 46 adult sheep. Analysis included absence or presence, number and location of subcutaneous nodules. Thoracic CT scans and pathological studies were prescribed to two further sheep. Single or multiple subcutaneous nodules were detected in 26 (56.52%) sheep. One or two nodules per animal were most often observed (36.95%). Size ranged between 1.5 and 4.5 cm. Pre-contrast two-dimensional (2D) CT images showed focal or multifocal increases in subcutaneous tissue thickness. Post-contrast 2D CT images revealed hypointense areas in the centre. Histopathology indicated the presence of granulomas composed by a large number of activated macrophages, surrounding a central core of necrosis. Large intracytoplasmic Al-positive aggregates were demonstrated by lumogallion staining. CT is a useful tool to detect subcutaneous Al hydroxide-induced granulomas in vivo in sheep. CT provides a diagnostic and research tool that can be very useful in future works in Al hydroxide-induced pathology, SRLV infection, or both. |
author2 |
Ministerio de Ciencia, Innovación y Universidades (España) |
author_facet |
Ministerio de Ciencia, Innovación y Universidades (España) Rodríguez-Largo, Ana Castells, Enrique Miguel, Ricardo de Gómez, Álex Ruiz, Héctor Pérez, Marta M. Reina, Ramsés Ferrer, Luis M. Luján, Lluís |
format |
artículo |
topic_facet |
Non-invasive diagnosis Ovine Small ruminant lentiviruses Subcutaneous granuloma |
author |
Rodríguez-Largo, Ana Castells, Enrique Miguel, Ricardo de Gómez, Álex Ruiz, Héctor Pérez, Marta M. Reina, Ramsés Ferrer, Luis M. Luján, Lluís |
author_sort |
Rodríguez-Largo, Ana |
title |
Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research |
title_short |
Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research |
title_full |
Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research |
title_fullStr |
Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research |
title_full_unstemmed |
Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research |
title_sort |
detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: a feasible approach for small ruminant lentiviruses diagnosis and research |
publisher |
John Wiley & Sons |
publishDate |
2021-09 |
url |
http://hdl.handle.net/10261/261408 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100010067 |
work_keys_str_mv |
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