Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research

Aluminium (Al) hydroxide use as adjuvant induces local formation of long-lasting subcutaneous granulomas in sheep. Macrophages within these granulomas have been identified as a new small ruminant lentivirus (SRLV) replication site in naturally infected animals. Diagnosis of Al hydroxide-induced granulomas in sheep is mostly based on postmortem observations but little information is available on in vivo detection. Computed tomography (CT) is used for studying these reactions in other animal species. To determine if CT could be a tool for in vivo diagnosis and research of subcutaneous Al hydroxide-induced granulomas in sheep. A retrospective survey on thoracic CT scans was performed on 46 adult sheep. Analysis included absence or presence, number and location of subcutaneous nodules. Thoracic CT scans and pathological studies were prescribed to two further sheep. Single or multiple subcutaneous nodules were detected in 26 (56.52%) sheep. One or two nodules per animal were most often observed (36.95%). Size ranged between 1.5 and 4.5 cm. Pre-contrast two-dimensional (2D) CT images showed focal or multifocal increases in subcutaneous tissue thickness. Post-contrast 2D CT images revealed hypointense areas in the centre. Histopathology indicated the presence of granulomas composed by a large number of activated macrophages, surrounding a central core of necrosis. Large intracytoplasmic Al-positive aggregates were demonstrated by lumogallion staining. CT is a useful tool to detect subcutaneous Al hydroxide-induced granulomas in vivo in sheep. CT provides a diagnostic and research tool that can be very useful in future works in Al hydroxide-induced pathology, SRLV infection, or both.

Saved in:
Bibliographic Details
Main Authors: Rodríguez-Largo, Ana, Castells, Enrique, Miguel, Ricardo de, Gómez, Álex, Ruiz, Héctor, Pérez, Marta M., Reina, Ramsés, Ferrer, Luis M., Luján, Lluís
Other Authors: Ministerio de Ciencia, Innovación y Universidades (España)
Format: artículo biblioteca
Published: John Wiley & Sons 2021-09
Subjects:Non-invasive diagnosis, Ovine, Small ruminant lentiviruses, Subcutaneous granuloma,
Online Access:http://hdl.handle.net/10261/261408
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100010067
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-idab-es-10261-261408
record_format koha
spelling dig-idab-es-10261-2614082022-02-22T02:22:26Z Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research Rodríguez-Largo, Ana Castells, Enrique Miguel, Ricardo de Gómez, Álex Ruiz, Héctor Pérez, Marta M. Reina, Ramsés Ferrer, Luis M. Luján, Lluís Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Gobierno de Aragón Non-invasive diagnosis Ovine Small ruminant lentiviruses Subcutaneous granuloma Aluminium (Al) hydroxide use as adjuvant induces local formation of long-lasting subcutaneous granulomas in sheep. Macrophages within these granulomas have been identified as a new small ruminant lentivirus (SRLV) replication site in naturally infected animals. Diagnosis of Al hydroxide-induced granulomas in sheep is mostly based on postmortem observations but little information is available on in vivo detection. Computed tomography (CT) is used for studying these reactions in other animal species. To determine if CT could be a tool for in vivo diagnosis and research of subcutaneous Al hydroxide-induced granulomas in sheep. A retrospective survey on thoracic CT scans was performed on 46 adult sheep. Analysis included absence or presence, number and location of subcutaneous nodules. Thoracic CT scans and pathological studies were prescribed to two further sheep. Single or multiple subcutaneous nodules were detected in 26 (56.52%) sheep. One or two nodules per animal were most often observed (36.95%). Size ranged between 1.5 and 4.5 cm. Pre-contrast two-dimensional (2D) CT images showed focal or multifocal increases in subcutaneous tissue thickness. Post-contrast 2D CT images revealed hypointense areas in the centre. Histopathology indicated the presence of granulomas composed by a large number of activated macrophages, surrounding a central core of necrosis. Large intracytoplasmic Al-positive aggregates were demonstrated by lumogallion staining. CT is a useful tool to detect subcutaneous Al hydroxide-induced granulomas in vivo in sheep. CT provides a diagnostic and research tool that can be very useful in future works in Al hydroxide-induced pathology, SRLV infection, or both. Spanish Ministry of Science, Innovation and Universities,Grant/Award Number:RTI2018- 096172-B-C33; Recognized ResearchGroups ofGovernment of Aragón, Grant/AwardNumber: A17_17R 2022-02-21T12:50:03Z 2022-02-21T12:50:03Z 2021-09 2022-02-21T12:50:03Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1002/vms3.572 e-issn: 2053-1095 Veterinary Medicine and Science 7(5): 1757-1761 (2021) http://hdl.handle.net/10261/261408 10.1002/vms3.572 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100010067 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096172-B-C33/ES/GRANULOMAS POSTVACUNALES EN OVINO: RELACION CON LENTIVIRUS DE LOS PEQUEÑOS RUMIANTES Y ESTUDIO DE UN ADYUVANTE VACUNAL ALTERNATIVO/ Publisher's version http://dx.doi.org/10.1002/vms3.572 Sí open John Wiley & Sons
institution IDAB ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-idab-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IDAB España
topic Non-invasive diagnosis
Ovine
Small ruminant lentiviruses
Subcutaneous granuloma
Non-invasive diagnosis
Ovine
Small ruminant lentiviruses
Subcutaneous granuloma
spellingShingle Non-invasive diagnosis
Ovine
Small ruminant lentiviruses
Subcutaneous granuloma
Non-invasive diagnosis
Ovine
Small ruminant lentiviruses
Subcutaneous granuloma
Rodríguez-Largo, Ana
Castells, Enrique
Miguel, Ricardo de
Gómez, Álex
Ruiz, Héctor
Pérez, Marta M.
Reina, Ramsés
Ferrer, Luis M.
Luján, Lluís
Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research
description Aluminium (Al) hydroxide use as adjuvant induces local formation of long-lasting subcutaneous granulomas in sheep. Macrophages within these granulomas have been identified as a new small ruminant lentivirus (SRLV) replication site in naturally infected animals. Diagnosis of Al hydroxide-induced granulomas in sheep is mostly based on postmortem observations but little information is available on in vivo detection. Computed tomography (CT) is used for studying these reactions in other animal species. To determine if CT could be a tool for in vivo diagnosis and research of subcutaneous Al hydroxide-induced granulomas in sheep. A retrospective survey on thoracic CT scans was performed on 46 adult sheep. Analysis included absence or presence, number and location of subcutaneous nodules. Thoracic CT scans and pathological studies were prescribed to two further sheep. Single or multiple subcutaneous nodules were detected in 26 (56.52%) sheep. One or two nodules per animal were most often observed (36.95%). Size ranged between 1.5 and 4.5 cm. Pre-contrast two-dimensional (2D) CT images showed focal or multifocal increases in subcutaneous tissue thickness. Post-contrast 2D CT images revealed hypointense areas in the centre. Histopathology indicated the presence of granulomas composed by a large number of activated macrophages, surrounding a central core of necrosis. Large intracytoplasmic Al-positive aggregates were demonstrated by lumogallion staining. CT is a useful tool to detect subcutaneous Al hydroxide-induced granulomas in vivo in sheep. CT provides a diagnostic and research tool that can be very useful in future works in Al hydroxide-induced pathology, SRLV infection, or both.
author2 Ministerio de Ciencia, Innovación y Universidades (España)
author_facet Ministerio de Ciencia, Innovación y Universidades (España)
Rodríguez-Largo, Ana
Castells, Enrique
Miguel, Ricardo de
Gómez, Álex
Ruiz, Héctor
Pérez, Marta M.
Reina, Ramsés
Ferrer, Luis M.
Luján, Lluís
format artículo
topic_facet Non-invasive diagnosis
Ovine
Small ruminant lentiviruses
Subcutaneous granuloma
author Rodríguez-Largo, Ana
Castells, Enrique
Miguel, Ricardo de
Gómez, Álex
Ruiz, Héctor
Pérez, Marta M.
Reina, Ramsés
Ferrer, Luis M.
Luján, Lluís
author_sort Rodríguez-Largo, Ana
title Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research
title_short Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research
title_full Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research
title_fullStr Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research
title_full_unstemmed Detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research
title_sort detection of aluminium hydroxide-induced granulomas in sheep by computed tomography: a feasible approach for small ruminant lentiviruses diagnosis and research
publisher John Wiley & Sons
publishDate 2021-09
url http://hdl.handle.net/10261/261408
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100010067
work_keys_str_mv AT rodriguezlargoana detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
AT castellsenrique detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
AT miguelricardode detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
AT gomezalex detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
AT ruizhector detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
AT perezmartam detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
AT reinaramses detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
AT ferrerluism detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
AT lujanlluis detectionofaluminiumhydroxideinducedgranulomasinsheepbycomputedtomographyafeasibleapproachforsmallruminantlentivirusesdiagnosisandresearch
_version_ 1777663536732831744