One evolutionarily selected amino acid variation is sufficient to provide functional specificity in the cold shock protein paralogs of Staphylococcus aureus
Bacterial genomes encode several families of protein paralogs. Discrimination between functional divergence and redundancy among paralogs is challenging due to their sequence conservation. Here, we investigated whether the amino acid differences present in the cold shock protein (CSP) paralogs of Staphylococcus aureus were responsible for functional specificity. Since deletion of cspA reduces the synthesis of staphyloxanthin (STX), we used it as an in vivo reporter of CSP functionality. Complementation of a ΔcspA strain with the different S. aureus CSP variants showed that only CspA could specifically restore STX production by controlling the activity of the stress-associated sigma B factor (σ). To determine the amino acid residues responsible for CspA specificity, we created several chimeric CSPs that interchanged the amino acid differences between CspA and CspC, which shared the highest identity. We demonstrated that CspA Pro58 was responsible for the specific control of σ activity and its associated phenotypes. Interestingly, CspC gained the biological function of CspA when the E58P substitution was introduced. This study highlights how just one evolutionarily selected amino acid change may be sufficient to modify the specific functionality of CSP paralogs.
Main Authors: | Catalán Moreno, Arancha, Caballero Sánchez, Carlos José, Irurzun, Naiara, Cuesta, Sergio, Lopez-Sagaseta, Jacinto, Toledo-Arana, Alejandro |
---|---|
Other Authors: | European Research Council |
Format: | artículo biblioteca |
Published: |
John Wiley & Sons
2020-04
|
Subjects: | Cold shock proteins, CspA, Protein paralogs, Protein specificity, RNA chaperone, Staphylococcus aureus, |
Online Access: | http://hdl.handle.net/10261/227978 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100000781 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100007680 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Fluorescent molecular beacons mimicking RNA secondary structures to study RNA chaperone activity
by: Menéndez Gil, Pilar, et al.
Published: (2020) -
Rol de la proteína A en la activación y regulación de la sobrevida de neutrófilos durante las infecciones por Staphylococcus aureus
by: Garófalo, Ailin Natalia -
DnaK/DnaJ-assisted recombinant protein production in Trichoplusia ni larvae
by: Martínez-Alonso, M., et al.
Published: (2010) -
Caracterización de la proteína Hsp90 y co-chaperonas de Toxoplasma gondii y su papel en el desarrollo del parásito
by: Echeverría, Pablo C. -
Dicoumarol down-regulates human PTTG1/Securin mRNA expression through inhibition of Hsp90
by: Hernández, Agustín, et al.
Published: (2008-03)