Studies on injection site reactions caused by aluminium-containing products in sheep
Trabajo presentado en el 3rd joint European Congress of the ESVP, ESTP and ECVP, celebrado en Lyon (Francia), del 30 de agosto al 2 de septiembre de 2017
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| Format: | comunicación de congreso biblioteca |
| Language: | English |
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2017-08-30
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| Online Access: | http://hdl.handle.net/10261/191915 |
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dig-idab-es-10261-1919152020-04-16T07:11:16Z Studies on injection site reactions caused by aluminium-containing products in sheep Asín, Javier Molín, Jéssica Pérez, Marta M. Pinczowski, Pedro Gimeno, Marina Navascués, Nuria Muniesa, Ana Blas, Ignacio de Lacasta, Delia Fernández, Antonio Pablo, Lorena de Mold, Matthew Exley, Christopher Andrés, Damián F. de Reina, Ramsés Luján, Lluís Trabajo presentado en el 3rd joint European Congress of the ESVP, ESTP and ECVP, celebrado en Lyon (Francia), del 30 de agosto al 2 de septiembre de 2017 Introduction: Aluminium (Al) containing adjuvants are widely used in sheep vaccines to promote an effective immune reaction against antigens but induce local injection-site reactions. This work aims to characterize these reactions and to determine the role of the Al in its genesis. Materials and Methods: 84 lambs divided into 3 groups (n=28 each) were inoculated with a different substance: A) Vaccines containing aluminium hydroxide; B) Aluminium hydroxide only; C) PBS. Animals received 19 subcutaneous inoculations along 15 months. Injection-site reactions and the regional lymph node (LN) were studied by gross and microscopic pathology, microbiology, fluorescence microscopy with lumogallion, Transmission Electron Microscopy (TEM), Energy Dispersive X-Ray Spectroscopy (EDS) and Graphite Furnace Atomic Absorption Spectroscopy (GFAAS). Results: Injection-site reactions consisted ofgranulomas that were more numerous (p<0.001) and with more severe central necrosis (p=0.021) in group A than in group B. Most of the vaccinated lambs (76.9%) showed more than 7 granulomas and all granulomas could be recovered in certain cases. Macrophages in the granulomas showed an orange fluorescence emission (590 nm), typical of Al. Similar groups of macrophages were observed in the regional LN. By TEM, macrophages in the granulomas contained aggregates of a spiculated electrondense material identified as Al by EDS. Group A showed longer Al particles than group B (p<0.001). By GFAAS, group A showed higher Al concentration in the regional LN than group B (p<0.001). Conclusions: Al induces persistent, immunomediated subcutaneous granulomas and the reactions are more severe in vaccinated animals. Al can reach the regional lymph nod Peer reviewed 2019-09-30T15:36:25Z 2019-09-30T15:36:25Z 2017-08-30 comunicación de congreso http://purl.org/coar/resource_type/c_5794 3rd joint European Congress of the ESVP, ESTP and ECVP (2017) http://hdl.handle.net/10261/191915 en Sí none |
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Trabajo presentado en el 3rd joint European Congress of the ESVP, ESTP and ECVP, celebrado en Lyon (Francia), del 30 de agosto al 2 de septiembre de 2017 |
| format |
comunicación de congreso |
| author |
Asín, Javier Molín, Jéssica Pérez, Marta M. Pinczowski, Pedro Gimeno, Marina Navascués, Nuria Muniesa, Ana Blas, Ignacio de Lacasta, Delia Fernández, Antonio Pablo, Lorena de Mold, Matthew Exley, Christopher Andrés, Damián F. de Reina, Ramsés Luján, Lluís |
| spellingShingle |
Asín, Javier Molín, Jéssica Pérez, Marta M. Pinczowski, Pedro Gimeno, Marina Navascués, Nuria Muniesa, Ana Blas, Ignacio de Lacasta, Delia Fernández, Antonio Pablo, Lorena de Mold, Matthew Exley, Christopher Andrés, Damián F. de Reina, Ramsés Luján, Lluís Studies on injection site reactions caused by aluminium-containing products in sheep |
| author_facet |
Asín, Javier Molín, Jéssica Pérez, Marta M. Pinczowski, Pedro Gimeno, Marina Navascués, Nuria Muniesa, Ana Blas, Ignacio de Lacasta, Delia Fernández, Antonio Pablo, Lorena de Mold, Matthew Exley, Christopher Andrés, Damián F. de Reina, Ramsés Luján, Lluís |
| author_sort |
Asín, Javier |
| title |
Studies on injection site reactions caused by aluminium-containing products in sheep |
| title_short |
Studies on injection site reactions caused by aluminium-containing products in sheep |
| title_full |
Studies on injection site reactions caused by aluminium-containing products in sheep |
| title_fullStr |
Studies on injection site reactions caused by aluminium-containing products in sheep |
| title_full_unstemmed |
Studies on injection site reactions caused by aluminium-containing products in sheep |
| title_sort |
studies on injection site reactions caused by aluminium-containing products in sheep |
| publishDate |
2017-08-30 |
| url |
http://hdl.handle.net/10261/191915 |
| work_keys_str_mv |
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