Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep
The use of vaccines including aluminum (Al)–based adjuvants is widespread among small ruminants and other animals. They are associated with the appearance of transient injection site nodules corresponding to granulomas. This study aims to characterize the morphology of these granulomas, to understand the role of the Al adjuvant in their genesis, and to establish the presence of the metal in regional lymph nodes. A total of 84 male neutered lambs were selected and divided into 3 treatment groups of 28 animals each: (1) vaccine (containing Al-based adjuvant), (2) adjuvant-only, and (3) control. A total of 19 subcutaneous injections were performed in a time frame of 15 months. Granulomas and regional lymph nodes were evaluated by clinicopathological means. All of the vaccine and 92.3% of the adjuvant-only lambs presented injection-site granulomas; the granulomas were more numerous in the group administered the vaccine. Bacterial culture in granulomas was always negative. Histologically, granulomas in the vaccine group presented a higher degree of severity. Al was specifically identified by lumogallion staining in granulomas and lymph nodes. Al median content was significantly higher (P < .001) in the lymph nodes of the vaccine group (82.65 μg/g) compared with both adjuvant-only (2.53 μg/g) and control groups (0.96 μg/g). Scanning transmission electron microscopy demonstrated aggregates of Al within macrophages in vaccine and adjuvant-only groups. In these two groups, Al-based adjuvants induce persistent, sterile, subcutaneous granulomas with macrophage-driven translocation of Al to regional lymph nodes. Local translocation of Al may induce further accumulation in distant tissues and be related to the appearance of systemic signs.
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Sage Publications
2019
|
Subjects: | Aluminum-based adjuvants, Granuloma, Macrophage, Sheep, Lymph node, Vaccine, |
Online Access: | http://hdl.handle.net/10261/191869 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-idab-es-10261-191869 |
---|---|
record_format |
koha |
spelling |
dig-idab-es-10261-1918692020-04-16T07:10:42Z Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep Asín, Javier Molín, Jéssica Pérez, Marta M. Pinczowski, Pedro Gimeno, Marina Navascués, Nuria Muniesa, Ana Blas, Ignacio de Lacasta, Delia Fernández, Antonio Pablo, Lorena de Mold, Matthew Exley, Christopher Andrés, Damián F. de Reina, Ramsés Luján, Lluís Mold, Matthew [0000-0002-4616-6204] Luján, Lluís [0000-0002-2053-9842] Aluminum-based adjuvants Granuloma Macrophage Sheep Lymph node Vaccine The use of vaccines including aluminum (Al)–based adjuvants is widespread among small ruminants and other animals. They are associated with the appearance of transient injection site nodules corresponding to granulomas. This study aims to characterize the morphology of these granulomas, to understand the role of the Al adjuvant in their genesis, and to establish the presence of the metal in regional lymph nodes. A total of 84 male neutered lambs were selected and divided into 3 treatment groups of 28 animals each: (1) vaccine (containing Al-based adjuvant), (2) adjuvant-only, and (3) control. A total of 19 subcutaneous injections were performed in a time frame of 15 months. Granulomas and regional lymph nodes were evaluated by clinicopathological means. All of the vaccine and 92.3% of the adjuvant-only lambs presented injection-site granulomas; the granulomas were more numerous in the group administered the vaccine. Bacterial culture in granulomas was always negative. Histologically, granulomas in the vaccine group presented a higher degree of severity. Al was specifically identified by lumogallion staining in granulomas and lymph nodes. Al median content was significantly higher (P < .001) in the lymph nodes of the vaccine group (82.65 μg/g) compared with both adjuvant-only (2.53 μg/g) and control groups (0.96 μg/g). Scanning transmission electron microscopy demonstrated aggregates of Al within macrophages in vaccine and adjuvant-only groups. In these two groups, Al-based adjuvants induce persistent, sterile, subcutaneous granulomas with macrophage-driven translocation of Al to regional lymph nodes. Local translocation of Al may induce further accumulation in distant tissues and be related to the appearance of systemic signs. Peer reviewed 2019-09-30T11:39:53Z 2019-09-30T11:39:53Z 2019 artículo http://purl.org/coar/resource_type/c_6501 Veterinary Pathology 56(3): 418–428 (2019) 1865-1674 0300-9858 http://hdl.handle.net/10261/191869 10.1177/0300985818809142 1544-2217 en https://doi.org/10.1177/0300985818809142 Sí none Sage Publications |
institution |
IDAB ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-idab-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IDAB España |
language |
English |
topic |
Aluminum-based adjuvants Granuloma Macrophage Sheep Lymph node Vaccine Aluminum-based adjuvants Granuloma Macrophage Sheep Lymph node Vaccine |
spellingShingle |
Aluminum-based adjuvants Granuloma Macrophage Sheep Lymph node Vaccine Aluminum-based adjuvants Granuloma Macrophage Sheep Lymph node Vaccine Asín, Javier Molín, Jéssica Pérez, Marta M. Pinczowski, Pedro Gimeno, Marina Navascués, Nuria Muniesa, Ana Blas, Ignacio de Lacasta, Delia Fernández, Antonio Pablo, Lorena de Mold, Matthew Exley, Christopher Andrés, Damián F. de Reina, Ramsés Luján, Lluís Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep |
description |
The use of vaccines including aluminum (Al)–based adjuvants is widespread among small ruminants and other animals. They are associated with the appearance of transient injection site nodules corresponding to granulomas. This study aims to characterize the morphology of these granulomas, to understand the role of the Al adjuvant in their genesis, and to establish the presence of the metal in regional lymph nodes. A total of 84 male neutered lambs were selected and divided into 3 treatment groups of 28 animals each: (1) vaccine (containing Al-based adjuvant), (2) adjuvant-only, and (3) control. A total of 19 subcutaneous injections were performed in a time frame of 15 months. Granulomas and regional lymph nodes were evaluated by clinicopathological means. All of the vaccine and 92.3% of the adjuvant-only lambs presented injection-site granulomas; the granulomas were more numerous in the group administered the vaccine. Bacterial culture in granulomas was always negative. Histologically, granulomas in the vaccine group presented a higher degree of severity. Al was specifically identified by lumogallion staining in granulomas and lymph nodes. Al median content was significantly higher (P < .001) in the lymph nodes of the vaccine group (82.65 μg/g) compared with both adjuvant-only (2.53 μg/g) and control groups (0.96 μg/g). Scanning transmission electron microscopy demonstrated aggregates of Al within macrophages in vaccine and adjuvant-only groups. In these two groups, Al-based adjuvants induce persistent, sterile, subcutaneous granulomas with macrophage-driven translocation of Al to regional lymph nodes. Local translocation of Al may induce further accumulation in distant tissues and be related to the appearance of systemic signs. |
author2 |
Mold, Matthew [0000-0002-4616-6204] |
author_facet |
Mold, Matthew [0000-0002-4616-6204] Asín, Javier Molín, Jéssica Pérez, Marta M. Pinczowski, Pedro Gimeno, Marina Navascués, Nuria Muniesa, Ana Blas, Ignacio de Lacasta, Delia Fernández, Antonio Pablo, Lorena de Mold, Matthew Exley, Christopher Andrés, Damián F. de Reina, Ramsés Luján, Lluís |
format |
artículo |
topic_facet |
Aluminum-based adjuvants Granuloma Macrophage Sheep Lymph node Vaccine |
author |
Asín, Javier Molín, Jéssica Pérez, Marta M. Pinczowski, Pedro Gimeno, Marina Navascués, Nuria Muniesa, Ana Blas, Ignacio de Lacasta, Delia Fernández, Antonio Pablo, Lorena de Mold, Matthew Exley, Christopher Andrés, Damián F. de Reina, Ramsés Luján, Lluís |
author_sort |
Asín, Javier |
title |
Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep |
title_short |
Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep |
title_full |
Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep |
title_fullStr |
Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep |
title_full_unstemmed |
Granulomas Following Subcutaneous Injection With Aluminum Adjuvant-Containing Products in Sheep |
title_sort |
granulomas following subcutaneous injection with aluminum adjuvant-containing products in sheep |
publisher |
Sage Publications |
publishDate |
2019 |
url |
http://hdl.handle.net/10261/191869 |
work_keys_str_mv |
AT asinjavier granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT molinjessica granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT perezmartam granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT pinczowskipedro granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT gimenomarina granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT navascuesnuria granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT muniesaana granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT blasignaciode granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT lacastadelia granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT fernandezantonio granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT pablolorenade granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT moldmatthew granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT exleychristopher granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT andresdamianfde granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT reinaramses granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep AT lujanlluis granulomasfollowingsubcutaneousinjectionwithaluminumadjuvantcontainingproductsinsheep |
_version_ |
1777663506334613504 |