Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route
The objective of this work was to comparatively study the tissue tropism and the associated pathology of 2 autochthonous small ruminant lentivirus (SRLV) field strains using an experimental infection in sheep through the bone marrow. Fifteen male, SRLV-free lambs of the Rasa Aragonesa breed were inoculated with strain 697 (nervous tissue origin, animals A1–A6), with strain 496 (articular origin, animals B1–B6), or with uninfected culture medium (C1–C3). Clinical, serologic, and polymerase chain reaction (PCR) evaluations were performed periodically. Two lambs from each infected group and a control animal were euthanized at 134, 273, and 319 days postinfection. Tissues were analyzed by gross and histopathologic evaluation; immunohistochemistry for CD3, CD4, CD8, CD68, and FoxP3 cell markers; lung morphometric evaluation; and tissue proviral quantification by PCR. All infected animals became positive either by enzyme-linked immunosorbent assay and/or PCR, with group B lambs showing the highest serologic values and more consistently positive PCR reactions. Group A lambs showed representative lung lesions but only mild histopathologic changes in the central nervous system (CNS) or in carpal joints. Contrarily, group B lambs demonstrated intense carpal arthritis and interstitial pneumonia but an absence of lesions in the CNS. Proviral copies in tissues were detected only in group B lambs. Experimental infection with these SRLV strains indicates that strain 496 is more virulent than strain 697 and more prone to induce arthritis, whereas strain 697 is more likely to reproduce encephalitis in Rasa Aragonesa lambs. Host factors as well as viral factors are responsible for the final clinicopathologic picture during SRLV infections.
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Format: | artículo biblioteca |
Language: | English |
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Sage Publications
2017-01-23
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Subjects: | Small ruminant lentivirus, Visna/maedi, Ovine progressive pneumonia, Experimental infection, Arthritis, Viral tropism, Sheep, Joint, |
Online Access: | http://hdl.handle.net/10261/191470 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003593 |
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dig-idab-es-10261-1914702019-09-27T10:58:57Z Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route Pinczowski, Pedro Sanjosé, Leticia Gimeno, Marina Crespo, Helena Glaria, Idoia Amorena Zabalza, Beatriz Andrés, Damián F. de Pérez, Marta M. Reina, Ramsés Luján, Lluís Ministerio de Ciencia e Innovación (España) Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) Small ruminant lentivirus Visna/maedi Ovine progressive pneumonia Experimental infection Arthritis Viral tropism Sheep Joint The objective of this work was to comparatively study the tissue tropism and the associated pathology of 2 autochthonous small ruminant lentivirus (SRLV) field strains using an experimental infection in sheep through the bone marrow. Fifteen male, SRLV-free lambs of the Rasa Aragonesa breed were inoculated with strain 697 (nervous tissue origin, animals A1–A6), with strain 496 (articular origin, animals B1–B6), or with uninfected culture medium (C1–C3). Clinical, serologic, and polymerase chain reaction (PCR) evaluations were performed periodically. Two lambs from each infected group and a control animal were euthanized at 134, 273, and 319 days postinfection. Tissues were analyzed by gross and histopathologic evaluation; immunohistochemistry for CD3, CD4, CD8, CD68, and FoxP3 cell markers; lung morphometric evaluation; and tissue proviral quantification by PCR. All infected animals became positive either by enzyme-linked immunosorbent assay and/or PCR, with group B lambs showing the highest serologic values and more consistently positive PCR reactions. Group A lambs showed representative lung lesions but only mild histopathologic changes in the central nervous system (CNS) or in carpal joints. Contrarily, group B lambs demonstrated intense carpal arthritis and interstitial pneumonia but an absence of lesions in the CNS. Proviral copies in tissues were detected only in group B lambs. Experimental infection with these SRLV strains indicates that strain 496 is more virulent than strain 697 and more prone to induce arthritis, whereas strain 697 is more likely to reproduce encephalitis in Rasa Aragonesa lambs. Host factors as well as viral factors are responsible for the final clinicopathologic picture during SRLV infections. The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: Grants by the Ministry of Science and Innovation of the Spanish Government, ref. No. AGL2010-22341-C04-02 and AGL2010-22341-C04-01. P. Pinczowski was a PhD student funded by the Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, ref. No. 202385/2011-6). R. Reina is supported by Spanish Ministry of Science and Innovation under a ‘Ramón y Cajal’ contract. Peer reviewed 2019-09-24T12:45:43Z 2019-09-24T12:45:43Z 2017-01-23 artículo http://purl.org/coar/resource_type/c_6501 Veterinary Pathology 54(3): 413-424 (2017) 0300-9858 http://hdl.handle.net/10261/191470 10.1177/0300985816688742 1544-2217 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003593 en https://doi.org/10.1177/0300985816688742 Sí none Sage Publications |
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Small ruminant lentivirus Visna/maedi Ovine progressive pneumonia Experimental infection Arthritis Viral tropism Sheep Joint Small ruminant lentivirus Visna/maedi Ovine progressive pneumonia Experimental infection Arthritis Viral tropism Sheep Joint |
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Small ruminant lentivirus Visna/maedi Ovine progressive pneumonia Experimental infection Arthritis Viral tropism Sheep Joint Small ruminant lentivirus Visna/maedi Ovine progressive pneumonia Experimental infection Arthritis Viral tropism Sheep Joint Pinczowski, Pedro Sanjosé, Leticia Gimeno, Marina Crespo, Helena Glaria, Idoia Amorena Zabalza, Beatriz Andrés, Damián F. de Pérez, Marta M. Reina, Ramsés Luján, Lluís Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route |
description |
The objective of this work was to comparatively study the tissue tropism and the associated pathology of 2 autochthonous small ruminant lentivirus (SRLV) field strains using an experimental infection in sheep through the bone marrow. Fifteen male, SRLV-free lambs of the Rasa Aragonesa breed were inoculated with strain 697 (nervous tissue origin, animals A1–A6), with strain 496 (articular origin, animals B1–B6), or with uninfected culture medium (C1–C3). Clinical, serologic, and polymerase chain reaction (PCR) evaluations were performed periodically. Two lambs from each infected group and a control animal were euthanized at 134, 273, and 319 days postinfection. Tissues were analyzed by gross and histopathologic evaluation; immunohistochemistry for CD3, CD4, CD8, CD68, and FoxP3 cell markers; lung morphometric evaluation; and tissue proviral quantification by PCR. All infected animals became positive either by enzyme-linked immunosorbent assay and/or PCR, with group B lambs showing the highest serologic values and more consistently positive PCR reactions. Group A lambs showed representative lung lesions but only mild histopathologic changes in the central nervous system (CNS) or in carpal joints. Contrarily, group B lambs demonstrated intense carpal arthritis and interstitial pneumonia but an absence of lesions in the CNS. Proviral copies in tissues were detected only in group B lambs. Experimental infection with these SRLV strains indicates that strain 496 is more virulent than strain 697 and more prone to induce arthritis, whereas strain 697 is more likely to reproduce encephalitis in Rasa Aragonesa lambs. Host factors as well as viral factors are responsible for the final clinicopathologic picture during SRLV infections. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Pinczowski, Pedro Sanjosé, Leticia Gimeno, Marina Crespo, Helena Glaria, Idoia Amorena Zabalza, Beatriz Andrés, Damián F. de Pérez, Marta M. Reina, Ramsés Luján, Lluís |
format |
artículo |
topic_facet |
Small ruminant lentivirus Visna/maedi Ovine progressive pneumonia Experimental infection Arthritis Viral tropism Sheep Joint |
author |
Pinczowski, Pedro Sanjosé, Leticia Gimeno, Marina Crespo, Helena Glaria, Idoia Amorena Zabalza, Beatriz Andrés, Damián F. de Pérez, Marta M. Reina, Ramsés Luján, Lluís |
author_sort |
Pinczowski, Pedro |
title |
Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route |
title_short |
Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route |
title_full |
Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route |
title_fullStr |
Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route |
title_full_unstemmed |
Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route |
title_sort |
small ruminant lentiviruses in sheep: pathology and tropism of 2 strains using the bone marrow route |
publisher |
Sage Publications |
publishDate |
2017-01-23 |
url |
http://hdl.handle.net/10261/191470 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003593 |
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