Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family

Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family

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Póster presentado en la X Reunión de Biología Molecular de plantas, celebrada en Valencia del 8 al 10 de julio de 2010.

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Main Authors: Bahaji, Abdellatif, Ovecka, Miroslav, Muñoz Pérez, Francisco José, Baroja-Fernández, Edurne, Montero, Manuel, Li, Jun, Sesma, María Teresa, Ezquer, Ignacio, Pozueta Romero, Javier
Format: póster de congreso biblioteca
Published: 2010-07
Online Access:http://hdl.handle.net/10261/141787
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spelling dig-idab-es-10261-1417872017-04-07T07:17:58Z Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family Bahaji, Abdellatif Ovecka, Miroslav Muñoz Pérez, Francisco José Baroja-Fernández, Edurne Montero, Manuel Li, Jun Sesma, María Teresa Ezquer, Ignacio Pozueta Romero, Javier Póster presentado en la X Reunión de Biología Molecular de plantas, celebrada en Valencia del 8 al 10 de julio de 2010. ZmBT1 and AtBT1 are members of the mitochondrial carrier family (MCF) that are suggested to be exclusively localized in the envelope membranes of plastids. ZmBT1 is thought to be involved in the transport to amyloplast of cytosolic ADPG linked to starch biosynthesis in maize endosperms, whereas AtBT1 appears to be involved in the export from plastids to the cytosol of newly synthesized adenylates in Arabidopsis. To investigate the impact of ZmBT1 and AtBT1 in plant metabolism, in this work we produced potato, barley and Arabidopsis plants ectopically expressing Zmbt1 and Atbt1. Zmbt1--expressing plants produced variegated leaves with reduced starch content, whereas Atbt1-expressing plants produced normal leaves accumulating wild type starch content. Homozygous Atbt1::T-DNA mutants displayed aberrant growth and sterility phenotype, which was complemented by Atbt1, but not by Zmbt1. The overall data thus indicated that (a) Zmbt1 and Atbt1 have different functions and (b) Atbt1 is an essential gene in Arabidopsis. Confocal fluorescence microscopy analyses of potato, Arabidopsis and maize plants stably expressing green fluorescent protein (GFP) fusions of ZmBT1 and AtBT1 revealed that the two proteins have dual localization to plastids and mitochondria. Furthermore, analyses of plants expressing GFP fusions of the ZmBT1 and AtBT1 N-terminal extensions, and of the mature proteins revealed that (a) AtBT1 and ZmBT1 N-terminal extensions comprise targeting sequences exclusively recognized by the plastidial compartment, and (b) targeting sequences to mitochondria are localized within the mature part of the protein. To the best of our knowledge this is the first report showing dual localization of MCF proteins to plastids and mitochondria. Based on these results, we propose that functions of ZmBT1 and AtBT1 should be re-evaluated. Peer Reviewed 2016-12-22T09:49:37Z 2016-12-22T09:49:37Z 2010-07 2016-12-22T09:49:38Z póster de congreso http://purl.org/coar/resource_type/c_6670 X Reunión de Biología Molecular de plantas (2010) http://hdl.handle.net/10261/141787 Pozueta Romero, Javier. Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family. http://doi.org/10.1093/pcp/pcr019 . http://hdl.handle.net/10261/51235 Sí none
institution IDAB ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-idab-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IDAB España
description Póster presentado en la X Reunión de Biología Molecular de plantas, celebrada en Valencia del 8 al 10 de julio de 2010.
format póster de congreso
author Bahaji, Abdellatif
Ovecka, Miroslav
Muñoz Pérez, Francisco José
Baroja-Fernández, Edurne
Montero, Manuel
Li, Jun
Sesma, María Teresa
Ezquer, Ignacio
Pozueta Romero, Javier
spellingShingle Bahaji, Abdellatif
Ovecka, Miroslav
Muñoz Pérez, Francisco José
Baroja-Fernández, Edurne
Montero, Manuel
Li, Jun
Sesma, María Teresa
Ezquer, Ignacio
Pozueta Romero, Javier
Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family
author_facet Bahaji, Abdellatif
Ovecka, Miroslav
Muñoz Pérez, Francisco José
Baroja-Fernández, Edurne
Montero, Manuel
Li, Jun
Sesma, María Teresa
Ezquer, Ignacio
Pozueta Romero, Javier
author_sort Bahaji, Abdellatif
title Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family
title_short Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family
title_full Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family
title_fullStr Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family
title_full_unstemmed Dual targeting to mitochondria and plastids of AtBT1 and ZmBT1, two members of the mitochondrial carrier family
title_sort dual targeting to mitochondria and plastids of atbt1 and zmbt1, two members of the mitochondrial carrier family
publishDate 2010-07
url http://hdl.handle.net/10261/141787
work_keys_str_mv AT bahajiabdellatif dualtargetingtomitochondriaandplastidsofatbt1andzmbt1twomembersofthemitochondrialcarrierfamily
AT oveckamiroslav dualtargetingtomitochondriaandplastidsofatbt1andzmbt1twomembersofthemitochondrialcarrierfamily
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