The presence of small ruminant lentiviruses in Mexican Pelibuey sheep
The transmission frequency of small ruminant lentiviruses (SRLVs) through the placenta is controversial and may be associated with breed susceptibility. In Mexico, SRLV infections in sheep have been poorly studied. This work explores the presence of antibodies and proviral DNA in Mexican Pelibuey sheep. Enzyme-linked immunosorbent assays (ELISAs; three commercial kits and two on the basis of synthetic peptides) and polymerase chain reaction (PCR; amplifying the long terminal repeat and gag segments) were performed to diagnose SRLV infection in 25 adult Pelibuey ewes with an average age of 2.5 years and 32 fetuses with gestational ages ranging from 40 to 90 days without clinical signs of SRLV. Two of the three commercial ELISAs and the synthetic peptide-based ones were positive for SRLV antibody detection in 28% and 24% of the ewes, respectively, whereas none of the fetuses were positive by any of the ELISAs. By PCR, 31% of the ewes and, interestingly, two fetuses were positive. Characteristic SRLV lesions were not found in the fetal and/or ewe tissues, including those with positive PCR results. These findings demonstrate the susceptibility of Pelibuey sheep to SRLV infection and the low transmission frequency through the placenta.
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Elsevier
2016-11
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Subjects: | Small ruminant lentiviruses, Synthetic peptides, Pelibuey breed, ELISA, PCR, |
Online Access: | http://hdl.handle.net/10261/141421 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100005739 |
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dig-idab-es-10261-1414212017-01-18T10:42:13Z The presence of small ruminant lentiviruses in Mexican Pelibuey sheep Sánchez, José H. Martínez, Humberto A. García, María M. García, María M. Garrido, Germán Gómez, Luis Aguilar, José A. Andrés, Damián F. de Reina, Ramsés Ramírez, Hugo Ministerio de Ciencia e Innovación (España) Universidad Nacional Autónoma de México Small ruminant lentiviruses Synthetic peptides Pelibuey breed ELISA PCR The transmission frequency of small ruminant lentiviruses (SRLVs) through the placenta is controversial and may be associated with breed susceptibility. In Mexico, SRLV infections in sheep have been poorly studied. This work explores the presence of antibodies and proviral DNA in Mexican Pelibuey sheep. Enzyme-linked immunosorbent assays (ELISAs; three commercial kits and two on the basis of synthetic peptides) and polymerase chain reaction (PCR; amplifying the long terminal repeat and gag segments) were performed to diagnose SRLV infection in 25 adult Pelibuey ewes with an average age of 2.5 years and 32 fetuses with gestational ages ranging from 40 to 90 days without clinical signs of SRLV. Two of the three commercial ELISAs and the synthetic peptide-based ones were positive for SRLV antibody detection in 28% and 24% of the ewes, respectively, whereas none of the fetuses were positive by any of the ELISAs. By PCR, 31% of the ewes and, interestingly, two fetuses were positive. Characteristic SRLV lesions were not found in the fetal and/or ewe tissues, including those with positive PCR results. These findings demonstrate the susceptibility of Pelibuey sheep to SRLV infection and the low transmission frequency through the placenta. This study was supported by the PIAPIC27/FESC/UNAM and FIS/IMSS/PROT/G12/1109 programs. Ramsés Reina was supported by the Spanish Ministry of Science and Innovation ‘Ramón y Cajal’ contract. Peer Reviewed 2016-12-14T11:33:11Z 2016-12-14T11:33:11Z 2016-11 2016-12-14T11:33:11Z artículo http://purl.org/coar/resource_type/c_6501 issn: 0093-691X Theriogenology 86(8): 1953-1957.e1 (2016) http://hdl.handle.net/10261/141421 10.1016/j.theriogenology.2016.06.017 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100005739 http://doi.org/10.1016/j.theriogenology.2016.06.017 Sí none Elsevier |
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Small ruminant lentiviruses Synthetic peptides Pelibuey breed ELISA PCR Small ruminant lentiviruses Synthetic peptides Pelibuey breed ELISA PCR |
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Small ruminant lentiviruses Synthetic peptides Pelibuey breed ELISA PCR Small ruminant lentiviruses Synthetic peptides Pelibuey breed ELISA PCR Sánchez, José H. Martínez, Humberto A. García, María M. García, María M. Garrido, Germán Gómez, Luis Aguilar, José A. Andrés, Damián F. de Reina, Ramsés Ramírez, Hugo The presence of small ruminant lentiviruses in Mexican Pelibuey sheep |
description |
The transmission frequency of small ruminant lentiviruses (SRLVs) through the placenta is controversial and may be associated with breed susceptibility. In Mexico, SRLV infections in sheep have been poorly studied. This work explores the presence of antibodies and proviral DNA in Mexican Pelibuey sheep. Enzyme-linked immunosorbent assays (ELISAs; three commercial kits and two on the basis of synthetic peptides) and polymerase chain reaction (PCR; amplifying the long terminal repeat and gag segments) were performed to diagnose SRLV infection in 25 adult Pelibuey ewes with an average age of 2.5 years and 32 fetuses with gestational ages ranging from 40 to 90 days without clinical signs of SRLV. Two of the three commercial ELISAs and the synthetic peptide-based ones were positive for SRLV antibody detection in 28% and 24% of the ewes, respectively, whereas none of the fetuses were positive by any of the ELISAs. By PCR, 31% of the ewes and, interestingly, two fetuses were positive. Characteristic SRLV lesions were not found in the fetal and/or ewe tissues, including those with positive PCR results. These findings demonstrate the susceptibility of Pelibuey sheep to SRLV infection and the low transmission frequency through the placenta. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Sánchez, José H. Martínez, Humberto A. García, María M. García, María M. Garrido, Germán Gómez, Luis Aguilar, José A. Andrés, Damián F. de Reina, Ramsés Ramírez, Hugo |
format |
artículo |
topic_facet |
Small ruminant lentiviruses Synthetic peptides Pelibuey breed ELISA PCR |
author |
Sánchez, José H. Martínez, Humberto A. García, María M. García, María M. Garrido, Germán Gómez, Luis Aguilar, José A. Andrés, Damián F. de Reina, Ramsés Ramírez, Hugo |
author_sort |
Sánchez, José H. |
title |
The presence of small ruminant lentiviruses in Mexican Pelibuey sheep |
title_short |
The presence of small ruminant lentiviruses in Mexican Pelibuey sheep |
title_full |
The presence of small ruminant lentiviruses in Mexican Pelibuey sheep |
title_fullStr |
The presence of small ruminant lentiviruses in Mexican Pelibuey sheep |
title_full_unstemmed |
The presence of small ruminant lentiviruses in Mexican Pelibuey sheep |
title_sort |
presence of small ruminant lentiviruses in mexican pelibuey sheep |
publisher |
Elsevier |
publishDate |
2016-11 |
url |
http://hdl.handle.net/10261/141421 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100005739 |
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