Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain
Reina, Ramsés et al.
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| Format: | artículo biblioteca |
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Elsevier
2006-11
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| Subjects: | Spain, Goats, Sheep, Caprine Arthritis Encephalitis, Maedi Visna, Phylogeny, pol, LTR, gag, Small ruminant lentiviruses, |
| Online Access: | http://hdl.handle.net/10261/114280 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100000780 |
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dig-idab-es-10261-1142802016-09-09T08:49:25Z Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain Reina, Ramsés Mora, María Isabel Glaria, Idoia García, Iker Solano Goñi, Cristina Amorena Zabalza, Beatriz Andrés, Damián F. de CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Comisión Interministerial de Ciencia y Tecnología, CICYT (España) European Commission Nafarroako Gobernua Spain Goats Sheep Caprine Arthritis Encephalitis Maedi Visna Phylogeny pol LTR gag Small ruminant lentiviruses Reina, Ramsés et al. Small ruminant lentiviruses (SRLV) are widely spread in many countries, including Spain. However, little is known about the genetic characteristics of Spanish goat and sheep SRLV. In this study, segments from three genomic regions (pol, gag-p25 and LTR) were amplified using DNA isolated from three Spanish autochthonous sheep (one) and goats (two). Animals (one per flock) belonged to distantly located, single-species flocks (goat or sheep). Sequence analysis showed conservation of regions that are putatively relevant to viral survival. Sequences of Spanish goat and sheep SRLV were allocated into phylogenetic trees (phylograms) with known SRLV groups. The phylograms corresponding to the pol, gag-p25 and LTR regions analyzed presented a compatible topology. This showed that Spanish caprine and ovine SRLV sequences belonged to the A or D phylogenetic groups and were closer to sheep SRLV prototypes (A1 group) than to goat SRLV prototypes (B or C groups), according to the current classification [Shah, C., Boni, J., Huder, J.B., Vogt, H.R., Muhlherr, J., Zanoni, R., Miserez, R., Lutz, H., Schupbach, J., 2004a. Phylogenetic analysis and reclassification of caprine and ovine lentiviruses based on 104 new isolates: evidence for regular sheep-to-goat transmission and worldwide propagation through livestock trade. Virology 319 (1), 12-26]. It was not possible to amplify in the three genetic regions the expected fragment in additional Spanish caprine and ovine SRLV proviral DNA sequences with the PCR primers used. This suggests that there is heterogeneity at the primer binding site among Spanish SRLV sequences. It also illustrates the need to develop diagnostic tests that are sensitive in local breeds. © 2006 Elsevier B.V. All rights reserved. This study was supported by grants from Spanish CICYT (AGL2000-1489-C02-01, AGL2003-08977-C03) and INIA (RTA01-107-C3-1); and the European Union (QLK2-CT-2002-00617 and EU LIFE-CRAFT No. CRAF-1999-70356). R.R. was supported by a fellowship from the Spanish CICYT. M.I.M. was supported by a grant from the Public University of Navarra. Peer Reviewed 2015-04-27T11:35:37Z 2015-04-27T11:35:37Z 2006-11 2015-04-27T11:35:38Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.virusres.2006.05.011 issn: 0168-1702 Virus Research 121(2): 189-198 (2006) http://hdl.handle.net/10261/114280 10.1016/j.virusres.2006.05.011 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.1016/j.virusres.2006.05.011 Sí none Elsevier |
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Spain Goats Sheep Caprine Arthritis Encephalitis Maedi Visna Phylogeny pol LTR gag Small ruminant lentiviruses Spain Goats Sheep Caprine Arthritis Encephalitis Maedi Visna Phylogeny pol LTR gag Small ruminant lentiviruses |
| spellingShingle |
Spain Goats Sheep Caprine Arthritis Encephalitis Maedi Visna Phylogeny pol LTR gag Small ruminant lentiviruses Spain Goats Sheep Caprine Arthritis Encephalitis Maedi Visna Phylogeny pol LTR gag Small ruminant lentiviruses Reina, Ramsés Mora, María Isabel Glaria, Idoia García, Iker Solano Goñi, Cristina Amorena Zabalza, Beatriz Andrés, Damián F. de Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain |
| description |
Reina, Ramsés et al. |
| author2 |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) |
| author_facet |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Reina, Ramsés Mora, María Isabel Glaria, Idoia García, Iker Solano Goñi, Cristina Amorena Zabalza, Beatriz Andrés, Damián F. de |
| format |
artículo |
| topic_facet |
Spain Goats Sheep Caprine Arthritis Encephalitis Maedi Visna Phylogeny pol LTR gag Small ruminant lentiviruses |
| author |
Reina, Ramsés Mora, María Isabel Glaria, Idoia García, Iker Solano Goñi, Cristina Amorena Zabalza, Beatriz Andrés, Damián F. de |
| author_sort |
Reina, Ramsés |
| title |
Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain |
| title_short |
Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain |
| title_full |
Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain |
| title_fullStr |
Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain |
| title_full_unstemmed |
Molecular characterization and phylogenetic study of Maedi Visna and Caprine Arthritis Encephalitis viral sequences in sheep and goats from Spain |
| title_sort |
molecular characterization and phylogenetic study of maedi visna and caprine arthritis encephalitis viral sequences in sheep and goats from spain |
| publisher |
Elsevier |
| publishDate |
2006-11 |
| url |
http://hdl.handle.net/10261/114280 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100000780 |
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