A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

[Background] The ABC (ATP-binding cassette) gene superfamily is widespread across all living species. The majority of ABC genes encode ABC transporters, which are membrane-spanning proteins capable of transferring substrates across biological membranes by hydrolyzing ATP. Although ABC transporters have often been associated with resistance to drugs and toxic compounds, within the Arthropoda ABC gene families have only been characterized in detail in several insects and a crustacean. In this study, we report a genome-wide survey and expression analysis of the ABC gene superfamily in the spider mite, Tetranychus urticae, a chelicerate ~ 450 million years diverged from other Arthropod lineages. T. urticae is a major agricultural pest, and is among of the most polyphagous arthropod herbivores known. The species resists a staggering array of toxic plant secondary metabolites, and has developed resistance to all major classes of pesticides in use for its control.

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Bibliographic Details
Main Authors: Dermauw, Wannes, Osborne, Edward J, Clark, Richard M., Grbić, Miodrag, Tirry, Luc, Van Leeuwen, Thomas
Other Authors: Ghent University
Format: artículo biblioteca
Language:English
Published: BioMed Central 2013-05-10
Subjects:Acari, RNA-seq, Microarray, Phase III detoxification, Duplication, Major facilitator superfamily,
Online Access:http://hdl.handle.net/10261/77653
http://dx.doi.org/10.13039/501100000023
http://dx.doi.org/10.13039/100008762
http://dx.doi.org/10.13039/501100000092
http://dx.doi.org/10.13039/100007747
http://dx.doi.org/10.13039/100000002
http://dx.doi.org/10.13039/501100003130
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id dig-icvv-es-10261-77653
record_format koha
institution ICVV ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icvv-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICVV España
language English
topic Acari
RNA-seq
Microarray
Phase III detoxification
Duplication
Major facilitator superfamily
Acari
RNA-seq
Microarray
Phase III detoxification
Duplication
Major facilitator superfamily
spellingShingle Acari
RNA-seq
Microarray
Phase III detoxification
Duplication
Major facilitator superfamily
Acari
RNA-seq
Microarray
Phase III detoxification
Duplication
Major facilitator superfamily
Dermauw, Wannes
Osborne, Edward J
Clark, Richard M.
Grbić, Miodrag
Tirry, Luc
Van Leeuwen, Thomas
A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae
description [Background] The ABC (ATP-binding cassette) gene superfamily is widespread across all living species. The majority of ABC genes encode ABC transporters, which are membrane-spanning proteins capable of transferring substrates across biological membranes by hydrolyzing ATP. Although ABC transporters have often been associated with resistance to drugs and toxic compounds, within the Arthropoda ABC gene families have only been characterized in detail in several insects and a crustacean. In this study, we report a genome-wide survey and expression analysis of the ABC gene superfamily in the spider mite, Tetranychus urticae, a chelicerate ~ 450 million years diverged from other Arthropod lineages. T. urticae is a major agricultural pest, and is among of the most polyphagous arthropod herbivores known. The species resists a staggering array of toxic plant secondary metabolites, and has developed resistance to all major classes of pesticides in use for its control.
author2 Ghent University
author_facet Ghent University
Dermauw, Wannes
Osborne, Edward J
Clark, Richard M.
Grbić, Miodrag
Tirry, Luc
Van Leeuwen, Thomas
format artículo
topic_facet Acari
RNA-seq
Microarray
Phase III detoxification
Duplication
Major facilitator superfamily
author Dermauw, Wannes
Osborne, Edward J
Clark, Richard M.
Grbić, Miodrag
Tirry, Luc
Van Leeuwen, Thomas
author_sort Dermauw, Wannes
title A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae
title_short A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae
title_full A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae
title_fullStr A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae
title_full_unstemmed A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae
title_sort burst of abc genes in the genome of the polyphagous spider mite tetranychus urticae
publisher BioMed Central
publishDate 2013-05-10
url http://hdl.handle.net/10261/77653
http://dx.doi.org/10.13039/501100000023
http://dx.doi.org/10.13039/100008762
http://dx.doi.org/10.13039/501100000092
http://dx.doi.org/10.13039/100007747
http://dx.doi.org/10.13039/100000002
http://dx.doi.org/10.13039/501100003130
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spelling dig-icvv-es-10261-776532021-12-27T15:51:12Z A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae Dermauw, Wannes Osborne, Edward J Clark, Richard M. Grbić, Miodrag Tirry, Luc Van Leeuwen, Thomas Ghent University Government of Canada Genome Canada Ontario Genomics Institute University of Utah National Institutes of Health (US) Research Foundation - Flanders Acari RNA-seq Microarray Phase III detoxification Duplication Major facilitator superfamily [Background] The ABC (ATP-binding cassette) gene superfamily is widespread across all living species. The majority of ABC genes encode ABC transporters, which are membrane-spanning proteins capable of transferring substrates across biological membranes by hydrolyzing ATP. Although ABC transporters have often been associated with resistance to drugs and toxic compounds, within the Arthropoda ABC gene families have only been characterized in detail in several insects and a crustacean. In this study, we report a genome-wide survey and expression analysis of the ABC gene superfamily in the spider mite, Tetranychus urticae, a chelicerate ~ 450 million years diverged from other Arthropod lineages. T. urticae is a major agricultural pest, and is among of the most polyphagous arthropod herbivores known. The species resists a staggering array of toxic plant secondary metabolites, and has developed resistance to all major classes of pesticides in use for its control. [Results] We identified 103 ABC genes in the T. urticae genome, the highest number discovered in a metazoan species to date. Within the T. urticae ABC gene set, all members of the eight currently described subfamilies (A to H) were detected. A phylogenetic analysis revealed that the high number of ABC genes in T. urticae is due primarily to lineage-specific expansions of ABC genes within the ABCC, ABCG and ABCH subfamilies. In particular, the ABCC subfamily harbors the highest number of T. urticae ABC genes (39). In a comparative genomic analysis, we found clear orthologous relationships between a subset of T. urticae ABC proteins and ABC proteins in both vertebrates and invertebrates known to be involved in fundamental cellular processes. These included members of the ABCB-half transporters, and the ABCD, ABCE and ABCF families. Furthermore, one-to-one orthologues could be distinguished between T. urticae proteins and human ABCC10, ABCG5 and ABCG8, the Drosophila melanogaster sulfonylurea receptor and ecdysone-regulated transporter E23. Finally, expression profiling revealed that ABC genes in the ABCC, ABCG ABCH subfamilies were differentially expressed in multi-pesticide resistant mite strains and/or in mites transferred to challenging (toxic) host plants. [Conclusions] In this study we present the first comprehensive analysis of ABC genes in a polyphagous arthropod herbivore. We demonstrate that the broad plant host range and high levels of pesticide resistance in T. urticae are associated with lineage-specific expansions of ABC genes, many of which respond transcriptionally to xenobiotic exposure. This ABC catalogue will serve as a basis for future biochemical and toxicological studies. Obtaining functional evidence that these ABC subfamilies contribute to xenobiotic tolerance should be the priority of future research. This work was supported by FWO grant 3G061011 (to TVL) and 3G009312 (to TVL and LT), a Ghent University Special Research Fund grant 01J13711 (to TVL and LT), the Government of Canada through Genome Canada and the Ontario Genomics Institute OGI-046 (to MG), a University of Utah Funding Incentive Seed Grant (to RMC), and a National Institutes of Health Genetics Training Grant T32 GM07464 (to EJO). Peer Reviewed 2013-06-06T05:18:37Z 2013-06-06T05:18:37Z 2013-05-10 2013-06-06T05:18:37Z artículo http://purl.org/coar/resource_type/c_6501 BMC Genomics 14(1): 317 (2013) 1471-2164 http://hdl.handle.net/10261/77653 10.1186/1471-2164-14-317 http://dx.doi.org/10.13039/501100000023 http://dx.doi.org/10.13039/100008762 http://dx.doi.org/10.13039/501100000092 http://dx.doi.org/10.13039/100007747 http://dx.doi.org/10.13039/100000002 http://dx.doi.org/10.13039/501100003130 23663308 en Publisher’s version http://dx.doi.org/10.1186/1471-2164-14-317 Sí open BioMed Central

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