Flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for KNR4/SMI1.
KNR4 defective recombinant wine yeast strains were previously shown to oversecrete mannoproteins during alcoholic fermentation and, depending on the genetic background, to contribute to protein stability of white wines. We have tried to get a deeper insight into the consequences of KNR4 deletion in a wine yeast strain, from both a biological and an enological standpoint, and to understand the mechanisms leading to improved mannoprotein release. In fermentation experiments, followed by aging on lees, and compared to the parent strain, the recombinant strain shows increased release of mannoproteins during the fermentation but little increase during aging. Mannoprotein release by the recombinant strain takes place mainly during the fermentation step. In contrast, autolysis of the recombinant strain keeps going after aging for 78 days. In addition, the recombinant strain is moderately flocculent, which would be interesting for the production of sparkling wines. This might be related to changes in the expression of Flo1p-regulated genes. The new biological processes affected by KNR4 deletion in wine yeasts, as revealed by this transcriptomic study are flocculation, adaptation to anaerobiosis, oxidative stress response, and ethanol tolerance, as well as FKS1 overexpression; but no overexpression was detected for genes coding for major structural mannoproteins of the cell wall. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012
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American Chemical Society
2011
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dig-icvv-es-10261-519692017-03-09T08:31:03Z Flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for KNR4/SMI1. Penacho, Vanessa Blondin, Bruno Valero, Eva González García, Ramón CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) KNR4 defective recombinant wine yeast strains were previously shown to oversecrete mannoproteins during alcoholic fermentation and, depending on the genetic background, to contribute to protein stability of white wines. We have tried to get a deeper insight into the consequences of KNR4 deletion in a wine yeast strain, from both a biological and an enological standpoint, and to understand the mechanisms leading to improved mannoprotein release. In fermentation experiments, followed by aging on lees, and compared to the parent strain, the recombinant strain shows increased release of mannoproteins during the fermentation but little increase during aging. Mannoprotein release by the recombinant strain takes place mainly during the fermentation step. In contrast, autolysis of the recombinant strain keeps going after aging for 78 days. In addition, the recombinant strain is moderately flocculent, which would be interesting for the production of sparkling wines. This might be related to changes in the expression of Flo1p-regulated genes. The new biological processes affected by KNR4 deletion in wine yeasts, as revealed by this transcriptomic study are flocculation, adaptation to anaerobiosis, oxidative stress response, and ethanol tolerance, as well as FKS1 overexpression; but no overexpression was detected for genes coding for major structural mannoproteins of the cell wall. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012 This work was supported by a predoctoral fellowship from INIA for V. P. (grant 18-BOE208 31-08-2006), the projects RTA2005-00169-00-00, AGL2006-02558, and the Picasso program (HF2006-0066). Peer Reviewed 2012-06-21T09:27:50Z 2012-06-21T09:27:50Z 2011 2012-06-21T09:27:50Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1002/btpr.734 issn: 1520-6033 Biotechnology Progress : - (2011) http://hdl.handle.net/10261/51969 10.1002/btpr.734 http://dx.doi.org/10.13039/100007652 en none American Chemical Society |
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KNR4 defective recombinant wine yeast strains were previously shown to oversecrete mannoproteins during alcoholic fermentation and, depending on the genetic background, to contribute to protein stability of white wines. We have tried to get a deeper insight into the consequences of KNR4 deletion in a wine yeast strain, from both a biological and an enological standpoint, and to understand the mechanisms leading to improved mannoprotein release. In fermentation experiments, followed by aging on lees, and compared to the parent strain, the recombinant strain shows increased release of mannoproteins during the fermentation but little increase during aging. Mannoprotein release by the recombinant strain takes place mainly during the fermentation step. In contrast, autolysis of the recombinant strain keeps going after aging for 78 days. In addition, the recombinant strain is moderately flocculent, which would be interesting for the production of sparkling wines. This might be related to changes in the expression of Flo1p-regulated genes. The new biological processes affected by KNR4 deletion in wine yeasts, as revealed by this transcriptomic study are flocculation, adaptation to anaerobiosis, oxidative stress response, and ethanol tolerance, as well as FKS1 overexpression; but no overexpression was detected for genes coding for major structural mannoproteins of the cell wall. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012 |
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CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) |
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CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Penacho, Vanessa Blondin, Bruno Valero, Eva González García, Ramón |
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Penacho, Vanessa Blondin, Bruno Valero, Eva González García, Ramón |
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Penacho, Vanessa Blondin, Bruno Valero, Eva González García, Ramón Flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for KNR4/SMI1. |
author_sort |
Penacho, Vanessa |
title |
Flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for KNR4/SMI1. |
title_short |
Flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for KNR4/SMI1. |
title_full |
Flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for KNR4/SMI1. |
title_fullStr |
Flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for KNR4/SMI1. |
title_full_unstemmed |
Flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for KNR4/SMI1. |
title_sort |
flocculation and transcriptional adaptation to fermentation conditions are modified in a recombinant wine yeast strain defective for knr4/smi1. |
publisher |
American Chemical Society |
publishDate |
2011 |
url |
http://hdl.handle.net/10261/51969 http://dx.doi.org/10.13039/100007652 |
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