Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp

Fernández, M. P.; Núñez, Yolanda; Ponz, Fernando; Hernáiz, Silvia; Gallego, F. J.; Ibáñez Marcos, Javier; CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)

Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp

11 páginas, 6 tablas.-- Part of this work was presented at the 9th International Conference on Grape Genetics and Breeding (2006) and will be published in Acta Horticulturae.

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Main Authors:	Fernández, M. P., Núñez, Yolanda, Ponz, Fernando, Hernáiz, Silvia, Gallego, F. J., Ibáñez Marcos, Javier
Other Authors:	CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Format:	artículo biblioteca
Language:	English
Published:	Springer 2008-10
Subjects:	DNA markers, Grapevine, Single base extension, Single nucleotide polymorphism, SSR,
Online Access:	http://hdl.handle.net/10261/37289 http://dx.doi.org/10.13039/100007652
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id	dig-icvv-es-10261-37289
record_format	koha
spelling	dig-icvv-es-10261-372892019-06-04T12:55:59Z Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp Fernández, M. P. Núñez, Yolanda Ponz, Fernando Hernáiz, Silvia Gallego, F. J. Ibáñez Marcos, Javier CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Superior Frutícola DNA markers Grapevine Single base extension Single nucleotide polymorphism SSR 11 páginas, 6 tablas.-- Part of this work was presented at the 9th International Conference on Grape Genetics and Breeding (2006) and will be published in Acta Horticulturae. Single nucleotide polymorphisms or SNPs are the most abundant form of genetic variation in the genome of plants and animals. Microsatellites are hypervariable regions of genome, while their flanking regions are assumed to be as conserved as the average of the genome. In the present study, flanking sequences of 10 microsatellite loci were compared in different cultivars of Vitis to determine the existing polymorphism. For every microsatellite, about 8 homozygous cultivars (regarding the microsatellite genotype) were chosen for sequencing. A total of 45 different varieties of Vitis and 91 sequences were analysed. Sequence polymorphisms were detected for all the microsatellite flanking regions studied, including single nucleotide polymorphisms (SNPs), insertions and deletions. The number of identified changes varied considerably among the loci with a frequency of one polymorphism every 41 nucleotides, being VVMD5 the most polymorphic one. A number of SNPs were used to design SNP markers, which were scored by dideoxy single base primer extension and capillary electrophoresis methodology. These SNP markers were employed to genotype 21 cultivars of Vitis vinifera and 4 varieties of other Vitis species. The utility of the markers developed as well as their utility for varietal identification and pedigree studies is discussed, using a similar study carried out with the 10 microsatellites as a reference. This research was financially supported by project CO-SF99 (funded by Superior Frutı´cola S.A.) and project AT06-004 (funded by INIA). Peer reviewed 2011-06-29T11:09:58Z 2011-06-29T11:09:58Z 2008-10 artículo http://purl.org/coar/resource_type/c_6501 Molecular Breeding 22(3): 455-465 82008) 1380-3743 http://hdl.handle.net/10261/37289 10.1007/s11032-008-9189-z http://dx.doi.org/10.13039/100007652 en http://dx.doi.org/10.1007/s11032-008-9189-z none Springer
institution	ICVV ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-icvv-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del ICVV España
language	English
topic	DNA markers Grapevine Single base extension Single nucleotide polymorphism SSR DNA markers Grapevine Single base extension Single nucleotide polymorphism SSR
spellingShingle	DNA markers Grapevine Single base extension Single nucleotide polymorphism SSR DNA markers Grapevine Single base extension Single nucleotide polymorphism SSR Fernández, M. P. Núñez, Yolanda Ponz, Fernando Hernáiz, Silvia Gallego, F. J. Ibáñez Marcos, Javier Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp
description	11 páginas, 6 tablas.-- Part of this work was presented at the 9th International Conference on Grape Genetics and Breeding (2006) and will be published in Acta Horticulturae.
author2	CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
author_facet	CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Fernández, M. P. Núñez, Yolanda Ponz, Fernando Hernáiz, Silvia Gallego, F. J. Ibáñez Marcos, Javier
format	artículo
topic_facet	DNA markers Grapevine Single base extension Single nucleotide polymorphism SSR
author	Fernández, M. P. Núñez, Yolanda Ponz, Fernando Hernáiz, Silvia Gallego, F. J. Ibáñez Marcos, Javier
author_sort	Fernández, M. P.
title	Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp
title_short	Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp
title_full	Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp
title_fullStr	Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp
title_full_unstemmed	Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp
title_sort	characterization of sequence polymorphisms from microsatellite flanking regions in vitis spp
publisher	Springer
publishDate	2008-10
url	http://hdl.handle.net/10261/37289 http://dx.doi.org/10.13039/100007652
work_keys_str_mv	AT fernandezmp characterizationofsequencepolymorphismsfrommicrosatelliteflankingregionsinvitisspp AT nunezyolanda characterizationofsequencepolymorphismsfrommicrosatelliteflankingregionsinvitisspp AT ponzfernando characterizationofsequencepolymorphismsfrommicrosatelliteflankingregionsinvitisspp AT hernaizsilvia characterizationofsequencepolymorphismsfrommicrosatelliteflankingregionsinvitisspp AT gallegofj characterizationofsequencepolymorphismsfrommicrosatelliteflankingregionsinvitisspp AT ibanezmarcosjavier characterizationofsequencepolymorphismsfrommicrosatelliteflankingregionsinvitisspp
_version_	1777670827617026048

Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp

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