Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype

Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype

Trabajo presentado en el International XI Symposium on Grapevine Physiology and Biotechnology, celebrado en Stellenbosch (Sudáfrica), del 31 de octubre al 5 de noviembre de 2021

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Main Authors: Alañón-Sánchez, Noelia, Carbonell-Bejerano, Pablo, Mauri, Nuria, Ferradas, Yolanda, Lijavetzky, Diego, Martínez-Zapater, José M., Ibáñez Marcos, Javier
Format: póster de congreso biblioteca
Published: 2021-10-31
Online Access:http://hdl.handle.net/10261/264311
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spelling dig-icvv-es-10261-2643112022-03-17T03:29:24Z Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype Alañón-Sánchez, Noelia Carbonell-Bejerano, Pablo Mauri, Nuria Ferradas, Yolanda Lijavetzky, Diego Martínez-Zapater, José M. Ibáñez Marcos, Javier Trabajo presentado en el International XI Symposium on Grapevine Physiology and Biotechnology, celebrado en Stellenbosch (Sudáfrica), del 31 de octubre al 5 de noviembre de 2021 Domesticated grapevine (Vitis vinifera L.) is used for wine, fresh fruit, raisins and juice production. Two subspecies can be identified within this species: V. vinifera ssp. vinifera, the cultivated form comprising mostly hermaphrodite and some female cultivars and V. vinifera ssp. sylvestris, the suggested wild dioecious ancestor. Studies dealing with this trait identified a major QTL on chromosome 2 as the grapevine Sex Determining Region (SDR), which harbours several proposed candidate genes. The aim of this work is the genetic and molecular characterization of a Tempranillo Tinto somatic variant that shows an androgenized flower phenotype. Whilst flowers in this somatic variant develop normal stamens, they present a reduced gynoecium that, unlike canonical male flowers of V. vinifera ssp. sylvestris, still enable fruit setting and ripening. Phenotyping results of a self-cross progeny of this variant line (more than 100 offspring) indicated that the mutant flower phenotype is inheritable. Furthermore, genotyping results of the microsatellite marker VVIB23, linked to the SDR, showed that the putative mutation co-localizes with this locus. One of the proposed female development inhibitor genes underlying the SDR locus is VviAPT3, which encodes an adenine phosphoribosyl transferase that may inactivate cytokinins by using them as substrate. The inactivation of these hormones, which promote gynoecium development in wild male vines if applied exogenously, could explain the mutant phenotype. RTqPCR and RNA-seq expression analyses during flower development demonstrated the overexpression of VviAPT3 in the mutant line compared to a normal flower Tempranillo Tinto line used as control. Several experiments are ongoing to identify the genetic variation that causes this male-like phenotype, such as the comparison of the whole genome sequences of the variant and a control Tempranillo line, or the genotyping of VviAPT3 and other candidate genes through Sanger sequencing. 2022-03-16T14:50:03Z 2022-03-16T14:50:03Z 2021-10-31 2022-03-16T14:50:04Z póster de congreso http://purl.org/coar/resource_type/c_6670 XI Symposium on Grapevine Physiology and Biotechnology (2021) http://hdl.handle.net/10261/264311 Sí none
institution ICVV ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icvv-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICVV España
description Trabajo presentado en el International XI Symposium on Grapevine Physiology and Biotechnology, celebrado en Stellenbosch (Sudáfrica), del 31 de octubre al 5 de noviembre de 2021
format póster de congreso
author Alañón-Sánchez, Noelia
Carbonell-Bejerano, Pablo
Mauri, Nuria
Ferradas, Yolanda
Lijavetzky, Diego
Martínez-Zapater, José M.
Ibáñez Marcos, Javier
spellingShingle Alañón-Sánchez, Noelia
Carbonell-Bejerano, Pablo
Mauri, Nuria
Ferradas, Yolanda
Lijavetzky, Diego
Martínez-Zapater, José M.
Ibáñez Marcos, Javier
Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype
author_facet Alañón-Sánchez, Noelia
Carbonell-Bejerano, Pablo
Mauri, Nuria
Ferradas, Yolanda
Lijavetzky, Diego
Martínez-Zapater, José M.
Ibáñez Marcos, Javier
author_sort Alañón-Sánchez, Noelia
title Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype
title_short Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype
title_full Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype
title_fullStr Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype
title_full_unstemmed Characterization of a cv. Tempranillo Tinto variant exhibiting a male-like flower phenotype
title_sort characterization of a cv. tempranillo tinto variant exhibiting a male-like flower phenotype
publishDate 2021-10-31
url http://hdl.handle.net/10261/264311
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