Prediction of protein assemblies, the next frontier: The CASP14-CAPRI experiment

We present the results for CAPRI Round 50, the fourth joint CASP-CAPRI protein assembly prediction challenge. The Round comprised a total of twelve targets, including six dimers, three trimers, and three higher-order oligomers. Four of these were easy targets, for which good structural templates were available either for the full assembly, or for the main interfaces (of the higher-order oligomers). Eight were difficult targets for which only distantly related templates were found for the individual subunits. Twenty-five CAPRI groups including eight automatic servers submitted ~1250 models per target. Twenty groups including six servers participated in the CAPRI scoring challenge submitted ~190 models per target. The accuracy of the predicted models was evaluated using the classical CAPRI criteria. The prediction performance was measured by a weighted scoring scheme that takes into account the number of models of acceptable quality or higher submitted by each group as part of their five top-ranking models. Compared to the previous CASP-CAPRI challenge, top performing groups submitted such models for a larger fraction (70–75%) of the targets in this Round, but fewer of these models were of high accuracy. Scorer groups achieved stronger performance with more groups submitting correct models for 70–80% of the targets or achieving high accuracy predictions. Servers performed less well in general, except for the MDOCKPP and LZERD servers, who performed on par with human groups. In addition to these results, major advances in methodology are discussed, providing an informative overview of where the prediction of protein assemblies currently stands.

Bibliographic Details

Main Authors:	Lensink, Marc F., Brysbaert, Guillaume, Mauri, Théo, Nadzirin, Nurul, Velankar, Sameer, Chaleil, Raphaël A. G., Clarence, Tereza, Bates, Paul A., Kong, Ren, Liu, Bin, Yang, Guangbo, Liu, Ming, Shi, Hang, Lu, Xufeng, Chang, Xang, Roy, Raj S., Quadir, Farhan, Liu, Jian, Cheng, Jianlin, Antoniak, Anna, Czaplewski, Cezary, Giełdón, Artur, Kogut, Mateusz, Lipska, Agnieszka, Liwo, Adam, Lubecka, Emilia, Maszota-Zieleniak, Martyna, Sieradzan, Adam K., Ślusarz, Rafał, Wesołowski, Patryk A., Zięba, Karolina, Carpio Muñoz, Carlos A. del, Ichiishi, Eiichiro, Harmalkar, Ameya, Gray, Jeffrey J., Bonvin, Alexandre M. J. J., Ambrosetti, Francesco, Vargas Honorato, Rodrigo, Jandova, Zuzana, Jiménez-García, Brian, Koukos, Panagiotis I., Keulen, Siri van, Noort, Charlotte W. van, Réau, Manon, Roel-Touris, Jorge, Kotelnikov, Sergey, Padhorny, Dzmitry, Porter, Kathryn, Alekseenko, Andrey, Ignatov, Mikhail, Desta, Israel, Ashizawa, Ryota, Sun, Zhuyezi, Ghani, Usman, Hashemi, Nasser, Vajda, Sandor, Kozakov, Dima, Rosell, Mireia, Rodríguez-Lumbreras, Luis A., Fernández-Recio, Juan, Karczynska, Agnieszka, Grudinin, Sergei, Yan, Yumeng, Li, Hao, Lin, Peicong, Huang, Sheng-You, Christoffer, Charles, Terashi, Genki, Verburgt, Jacob, Sarkar, Daipayan, Aderinwale, Tunde, Wang, Xiao, Kihara, Daisuke, Nakamura, Tsukasa, Hanazono, Huya, Gowthaman, Ragul, Guest, Johnathan D., Yin, Rui, Taherzadeh, Ghazaleh, Pierce, Brian G., Barradas-Bautista, Didier, Cao, Zhen, Cavallo, Luigi, Oliva, Romina, Sun, Yuanfei, Zhu, Shaowen, Shen, Yang, Park, Taeyong, Woo, Hyeonuk, Yang, Jinsol, Kwon, Sohee, Won, Jonghun, Seok, Chaok, Kiyota, Yasuomi, Kobayashi, Shinpei, Harada, Yoshiki, Takeda-Shitaka, Mayuko, Kundrotas, Petras J., Singh, Amar, Vakser, Ilya A., Dapkunas, Justas, Olechnovic, Kliment, Venclovas, Česlovas, Duan, Rui, Qiu, Liming, Xu, Xianjin, Zhang, Shuang, Zou, Xiaoqin, Wodak, Shoshana J.
Other Authors:	Cancer Research UK
Format:	artículo biblioteca
Published:	Wiley-Liss 2021-12
Subjects:	Blind prediction, CAPRI, CASP, Docking, Oligomeric state, Protein assemblies, Protein complexes, Protein docking, Protein–protein interaction, Template-based modeling,
Online Access:	http://hdl.handle.net/10261/262789 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100008428 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012950 http://dx.doi.org/10.13039/501100001691 http://dx.doi.org/10.13039/501100000265 http://dx.doi.org/10.13039/100000057 http://dx.doi.org/10.13039/100000002 http://dx.doi.org/10.13039/501100001809 http://dx.doi.org/10.13039/100000001 http://dx.doi.org/10.13039/501100000289
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