Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum
Grapevine trunk diseases (GTDs) are a big threat for global viticulture. Without effective chemicals, biocontrol strategies are developed as alternatives to better cope with environmental concerns. A combination of biological control agents (BCAs) could even improve sustainable disease management through complementary ways of protection. In this study, we evaluated the combination of Bacillus subtilis (Bs) PTA-271 and Trichoderma atroviride (Ta) SC1 for the protection of Chardonnay and Tempranillo rootlings against Neofusicoccum parvum Bt67, an aggressive pathogen associated to Botryosphaeria dieback (BD). Indirect benefits offered by each BCA and their combination were then characterized in planta, as well as their direct benefits in vitro. Results provide evidence that (1) the cultivar contributes to the beneficial effects of Bs PTA-271 and Ta SC1 against N. parvum, and that (2) the in vitro BCA mutual antagonism switches to the strongest fungistatic effect toward Np-Bt67 in a three-way confrontation test. We also report for the first time the beneficial potential of a combination of BCA against Np-Bt67 especially in Tempranillo. Our findings highlight a common feature for both cultivars: salicylic acid (SA)-dependent defenses were strongly decreased in plants protected by the BCA, in contrast with symptomatic ones. We thus suggest that (1) the high basal expression of SA-dependent defenses in Tempranillo explains its highest susceptibility to N. parvum, and that (2) the cultivar-specific responses to the beneficial Bs PTA-271 and Ta SC1 remain to be further investigated.
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Frontiers Media
2021-10-14
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Subjects: | Chardonnay, Tempranillo, Biocontrol, Neofusicoccum parvum, Plant immunity, Synergistic effect, |
Online Access: | http://hdl.handle.net/10261/262498 http://dx.doi.org/10.13039/501100011033 |
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dig-icvv-es-10261-2624982022-03-03T07:25:22Z Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum Leal, Catarina Richet; Nicolas Guise, Jean-François Gramaje, David Armengol, Josep Fontaine, Florence Trotel-Aziz, Patricia Région Grand Est (France) Communauté urbaine du Grand Reims Belchim Crop Protection Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Chardonnay Tempranillo Biocontrol Neofusicoccum parvum Plant immunity Synergistic effect Grapevine trunk diseases (GTDs) are a big threat for global viticulture. Without effective chemicals, biocontrol strategies are developed as alternatives to better cope with environmental concerns. A combination of biological control agents (BCAs) could even improve sustainable disease management through complementary ways of protection. In this study, we evaluated the combination of Bacillus subtilis (Bs) PTA-271 and Trichoderma atroviride (Ta) SC1 for the protection of Chardonnay and Tempranillo rootlings against Neofusicoccum parvum Bt67, an aggressive pathogen associated to Botryosphaeria dieback (BD). Indirect benefits offered by each BCA and their combination were then characterized in planta, as well as their direct benefits in vitro. Results provide evidence that (1) the cultivar contributes to the beneficial effects of Bs PTA-271 and Ta SC1 against N. parvum, and that (2) the in vitro BCA mutual antagonism switches to the strongest fungistatic effect toward Np-Bt67 in a three-way confrontation test. We also report for the first time the beneficial potential of a combination of BCA against Np-Bt67 especially in Tempranillo. Our findings highlight a common feature for both cultivars: salicylic acid (SA)-dependent defenses were strongly decreased in plants protected by the BCA, in contrast with symptomatic ones. We thus suggest that (1) the high basal expression of SA-dependent defenses in Tempranillo explains its highest susceptibility to N. parvum, and that (2) the cultivar-specific responses to the beneficial Bs PTA-271 and Ta SC1 remain to be further investigated. This work was supported by a French Grant from the Region GRAND-EST France and the City of GRAND-REIMS France through the BIOVIGNE Ph.D. program, whose functioning is supported by BELCHIM Crop Protection France. DG was supported by the Ramón y Cajal program, Spanish Government (RyC-2017-23098). 2022-03-02T10:10:18Z 2022-03-02T10:10:18Z 2021-10-14 2022-03-02T10:10:18Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.3389/fmicb.2021.726132 e-issn: 1664-302X Frontiers in Microbiology 12: 726132 (2021) http://hdl.handle.net/10261/262498 10.3389/fmicb.2021.726132 http://dx.doi.org/10.13039/501100011033 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI//RYC-2017-23098 Publisher's version http://dx.doi.org/10.3389/fmicb.2021.726132 Sí open Frontiers Media |
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Chardonnay Tempranillo Biocontrol Neofusicoccum parvum Plant immunity Synergistic effect Chardonnay Tempranillo Biocontrol Neofusicoccum parvum Plant immunity Synergistic effect |
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Chardonnay Tempranillo Biocontrol Neofusicoccum parvum Plant immunity Synergistic effect Chardonnay Tempranillo Biocontrol Neofusicoccum parvum Plant immunity Synergistic effect Leal, Catarina Richet; Nicolas Guise, Jean-François Gramaje, David Armengol, Josep Fontaine, Florence Trotel-Aziz, Patricia Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum |
description |
Grapevine trunk diseases (GTDs) are a big threat for global viticulture. Without effective chemicals, biocontrol strategies are developed as alternatives to better cope with environmental concerns. A combination of biological control agents (BCAs) could even improve sustainable disease management through complementary ways of protection. In this study, we evaluated the combination of Bacillus subtilis (Bs) PTA-271 and Trichoderma atroviride (Ta) SC1 for the protection of Chardonnay and Tempranillo rootlings against Neofusicoccum parvum Bt67, an aggressive pathogen associated to Botryosphaeria dieback (BD). Indirect benefits offered by each BCA and their combination were then characterized in planta, as well as their direct benefits in vitro. Results provide evidence that (1) the cultivar contributes to the beneficial effects of Bs PTA-271 and Ta SC1 against N. parvum, and that (2) the in vitro BCA mutual antagonism switches to the strongest fungistatic effect toward Np-Bt67 in a three-way confrontation test. We also report for the first time the beneficial potential of a combination of BCA against Np-Bt67 especially in Tempranillo. Our findings highlight a common feature for both cultivars: salicylic acid (SA)-dependent defenses were strongly decreased in plants protected by the BCA, in contrast with symptomatic ones. We thus suggest that (1) the high basal expression of SA-dependent defenses in Tempranillo explains its highest susceptibility to N. parvum, and that (2) the cultivar-specific responses to the beneficial Bs PTA-271 and Ta SC1 remain to be further investigated. |
author2 |
Région Grand Est (France) |
author_facet |
Région Grand Est (France) Leal, Catarina Richet; Nicolas Guise, Jean-François Gramaje, David Armengol, Josep Fontaine, Florence Trotel-Aziz, Patricia |
format |
artículo |
topic_facet |
Chardonnay Tempranillo Biocontrol Neofusicoccum parvum Plant immunity Synergistic effect |
author |
Leal, Catarina Richet; Nicolas Guise, Jean-François Gramaje, David Armengol, Josep Fontaine, Florence Trotel-Aziz, Patricia |
author_sort |
Leal, Catarina |
title |
Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum |
title_short |
Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum |
title_full |
Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum |
title_fullStr |
Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum |
title_full_unstemmed |
Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum |
title_sort |
cultivar contributes to the beneficial effects of bacillus subtilis pta-271 and trichoderma atroviride sc1 to protect grapevine against neofusicoccum parvum |
publisher |
Frontiers Media |
publishDate |
2021-10-14 |
url |
http://hdl.handle.net/10261/262498 http://dx.doi.org/10.13039/501100011033 |
work_keys_str_mv |
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