Composition and biological activity of the Algerian plant Rosa canina L. by HPLC-UV-MS
The present study was carried out in order to identify and characterize the compounds of Rosa canina fruits by HPLC-UV-MS. The total phenolic determiner by a new Fast Blue method (FBBB), which detects phenolic directly, reported an average total phenolic concentration of 1.7 folds greater than Folin-Ciocalteu (F-C), which indicates that an indirect detection method of total phenolic should be replaced in future studies by the FBBB method. TPC of the ethanolic extract was positively correlated with 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging effect. The DPPH activity of R. canina extract which is higher than the IC50 of the ascorbic acid and Butylated Hydroxytoluene (BHT), but lower than the IC50 of quercetin and trolox. The determination of intracellular reactive oxygen species (ROS) proved the antioxidant effect of the extract on HepG2 and SH-SY5Y cells. A concentration of 1.63 μg/ml on HepG2 cells had an oxidizing effect instead of the antioxidant effect, which is due to the existence of a tert-butyl group in sesquiterpene identified by HPLC-UV-MS method. These results indicate that the fruits of R. canina can be used as a natural source of antioxidants against oxidative stress and some types of cancer.
Main Authors: | , , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2020
|
Subjects: | Ethanolic extract, Bilobalide A, HPLC-UV-MS, Antioxidant activity, Oxidant effect, |
Online Access: | http://hdl.handle.net/10261/219345 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-icvv-es-10261-219345 |
---|---|
record_format |
koha |
spelling |
dig-icvv-es-10261-2193452021-06-30T10:52:42Z Composition and biological activity of the Algerian plant Rosa canina L. by HPLC-UV-MS Fetni, Samira Bertella, Nabil Ouahab, Ammar Martínez-Zapater, José M. Pascual-Teresa, Sonia de Ethanolic extract Bilobalide A HPLC-UV-MS Antioxidant activity Oxidant effect The present study was carried out in order to identify and characterize the compounds of Rosa canina fruits by HPLC-UV-MS. The total phenolic determiner by a new Fast Blue method (FBBB), which detects phenolic directly, reported an average total phenolic concentration of 1.7 folds greater than Folin-Ciocalteu (F-C), which indicates that an indirect detection method of total phenolic should be replaced in future studies by the FBBB method. TPC of the ethanolic extract was positively correlated with 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging effect. The DPPH activity of R. canina extract which is higher than the IC50 of the ascorbic acid and Butylated Hydroxytoluene (BHT), but lower than the IC50 of quercetin and trolox. The determination of intracellular reactive oxygen species (ROS) proved the antioxidant effect of the extract on HepG2 and SH-SY5Y cells. A concentration of 1.63 μg/ml on HepG2 cells had an oxidizing effect instead of the antioxidant effect, which is due to the existence of a tert-butyl group in sesquiterpene identified by HPLC-UV-MS method. These results indicate that the fruits of R. canina can be used as a natural source of antioxidants against oxidative stress and some types of cancer. Peer reviewed 2020-09-09T09:33:44Z 2020-09-09T09:33:44Z 2020 artículo http://purl.org/coar/resource_type/c_6501 Arabian Journal of Chemistry 13(1): 1105-1119 (2020) 1878-5352 http://hdl.handle.net/10261/219345 10.1016/j.arabjc.2017.09.013 en Publisher's version https://doi.org/10.1016/j.arabjc.2017.09.013 Sí open Elsevier |
institution |
ICVV ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-icvv-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del ICVV España |
language |
English |
topic |
Ethanolic extract Bilobalide A HPLC-UV-MS Antioxidant activity Oxidant effect Ethanolic extract Bilobalide A HPLC-UV-MS Antioxidant activity Oxidant effect |
spellingShingle |
Ethanolic extract Bilobalide A HPLC-UV-MS Antioxidant activity Oxidant effect Ethanolic extract Bilobalide A HPLC-UV-MS Antioxidant activity Oxidant effect Fetni, Samira Bertella, Nabil Ouahab, Ammar Martínez-Zapater, José M. Pascual-Teresa, Sonia de Composition and biological activity of the Algerian plant Rosa canina L. by HPLC-UV-MS |
description |
The present study was carried out in order to identify and characterize the compounds of Rosa canina fruits by HPLC-UV-MS. The total phenolic determiner by a new Fast Blue method (FBBB), which detects phenolic directly, reported an average total phenolic concentration of 1.7 folds greater than Folin-Ciocalteu (F-C), which indicates that an indirect detection method of total phenolic should be replaced in future studies by the FBBB method. TPC of the ethanolic extract was positively correlated with 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging effect. The DPPH activity of R. canina extract which is higher than the IC50 of the ascorbic acid and Butylated Hydroxytoluene (BHT), but lower than the IC50 of quercetin and trolox. The determination of intracellular reactive oxygen species (ROS) proved the antioxidant effect of the extract on HepG2 and SH-SY5Y cells. A concentration of 1.63 μg/ml on HepG2 cells had an oxidizing effect instead of the antioxidant effect, which is due to the existence of a tert-butyl group in sesquiterpene identified by HPLC-UV-MS method. These results indicate that the fruits of R. canina can be used as a natural source of antioxidants against oxidative stress and some types of cancer. |
format |
artículo |
topic_facet |
Ethanolic extract Bilobalide A HPLC-UV-MS Antioxidant activity Oxidant effect |
author |
Fetni, Samira Bertella, Nabil Ouahab, Ammar Martínez-Zapater, José M. Pascual-Teresa, Sonia de |
author_facet |
Fetni, Samira Bertella, Nabil Ouahab, Ammar Martínez-Zapater, José M. Pascual-Teresa, Sonia de |
author_sort |
Fetni, Samira |
title |
Composition and biological activity of the Algerian plant Rosa canina L. by HPLC-UV-MS |
title_short |
Composition and biological activity of the Algerian plant Rosa canina L. by HPLC-UV-MS |
title_full |
Composition and biological activity of the Algerian plant Rosa canina L. by HPLC-UV-MS |
title_fullStr |
Composition and biological activity of the Algerian plant Rosa canina L. by HPLC-UV-MS |
title_full_unstemmed |
Composition and biological activity of the Algerian plant Rosa canina L. by HPLC-UV-MS |
title_sort |
composition and biological activity of the algerian plant rosa canina l. by hplc-uv-ms |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/219345 |
work_keys_str_mv |
AT fetnisamira compositionandbiologicalactivityofthealgerianplantrosacaninalbyhplcuvms AT bertellanabil compositionandbiologicalactivityofthealgerianplantrosacaninalbyhplcuvms AT ouahabammar compositionandbiologicalactivityofthealgerianplantrosacaninalbyhplcuvms AT martinezzapaterjosem compositionandbiologicalactivityofthealgerianplantrosacaninalbyhplcuvms AT pascualteresasoniade compositionandbiologicalactivityofthealgerianplantrosacaninalbyhplcuvms |
_version_ |
1777670995159547904 |