Vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: Evidence from the Algarve, Portugal
Entomopathogenic nematodes (EPNs) are widely distributed in soils throughout the world. Their activity as biological control agents is modulated by abiotic and biotic factors (e.g. soil type, climatic fluctuation and natural enemies). We sought to identify soil properties in a Mediterranean region, which might be managed to enhance biological control agents’ services provided by EPNs. We hypothesized that responses of EPN soil food web assemblages to abiotic factors in such a Mediterranean region would be consistent with previous observations in other biomes in subtropical and temperate regions, in which pH and variables related to water content were main drivers of such association. We also expected that EPN abundance and species composition would differ between stable botanical habitats (citrus groves, palmetto areas, oaks and pines), with EPNs and associated organisms favoured in cultivated sites (citrus). In spring 2016, 50 georeferenced localities, representing four botanical habitats and two soil-ecoregions (calcareous versus non-calcareous), were surveyed. Using published and de novo real time qPCR tools, we evaluated the frequency and abundance of 10 EPN species and 13 organisms associated with EPNs: 6 nematophagous fungi (NF), 5 free-living nematodes (FLN), and 2 ectoparasitic bacteria. EPN activity was also assessed by traditional insect-baiting, allowing the evaluation of FLN-EPN mixed progeny. EPNs were detected by qPCR in 50% of localities, and strongly correlated with EPN activity. Steinernema feltiae was the dominant EPN species measured by both techniques (qPCR and insect-bait), being widespread in all Algarve, while Heterorhabditis bacteriophora was detected mainly in citrus groves. The species S. arenarium and H. indica were detected by qPCR for the first time in continental Portugal. The molecular analysis of insect cadaver progeny revealed novel FLN-EPN associations with Pristionchus maupasi and P. pacificus. EPN, FLN and NF abundance differed among botanical groups, with citrus groves supporting high numbers of all trophic guilds. Oaks also favoured EPNs. Similarly, calcareous soil-ecoregion supported higher NF, FLN and EPN abundance. Two abiotic variables (pH, and clay content) explained the community variation in multivariate analysis, consistent with key abiotic variables described for other subtropical and temperate regions. The results supported the hypothesis that cultivated perennial habitats favour EPNs and soil organisms that can limit EPN activity as biological control agents.
Main Authors: | , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2019-01
|
Subjects: | Entomopathogenic nematodes, Habitat preference, Mediterranean agro-ecosystems, Multivariate analysis, Quantitative real-time PCR, |
Online Access: | http://hdl.handle.net/10261/193812 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100003329 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-icvv-es-10261-193812 |
---|---|
record_format |
koha |
institution |
ICVV ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-icvv-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del ICVV España |
language |
English |
topic |
Entomopathogenic nematodes Habitat preference Mediterranean agro-ecosystems Multivariate analysis Quantitative real-time PCR Entomopathogenic nematodes Habitat preference Mediterranean agro-ecosystems Multivariate analysis Quantitative real-time PCR |
spellingShingle |
Entomopathogenic nematodes Habitat preference Mediterranean agro-ecosystems Multivariate analysis Quantitative real-time PCR Entomopathogenic nematodes Habitat preference Mediterranean agro-ecosystems Multivariate analysis Quantitative real-time PCR Campos-Herrera, R. Blanco-Pérez, Rubén Bueno-Pallero, Francisco Ángel Duarte, Amílcar Nolasco, Gustavo Sommer, Ralf J. Rodríguez Martín, José Antonio Vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: Evidence from the Algarve, Portugal |
description |
Entomopathogenic nematodes (EPNs) are widely distributed in soils throughout the world. Their activity as biological control agents is modulated by abiotic and biotic factors (e.g. soil type, climatic fluctuation and natural enemies). We sought to identify soil properties in a Mediterranean region, which might be managed to enhance biological control agents’ services provided by EPNs. We hypothesized that responses of EPN soil food web assemblages to abiotic factors in such a Mediterranean region would be consistent with previous observations in other biomes in subtropical and temperate regions, in which pH and variables related to water content were main drivers of such association. We also expected that EPN abundance and species composition would differ between stable botanical habitats (citrus groves, palmetto areas, oaks and pines), with EPNs and associated organisms favoured in cultivated sites (citrus). In spring 2016, 50 georeferenced localities, representing four botanical habitats and two soil-ecoregions (calcareous versus non-calcareous), were surveyed. Using published and de novo real time qPCR tools, we evaluated the frequency and abundance of 10 EPN species and 13 organisms associated with EPNs: 6 nematophagous fungi (NF), 5 free-living nematodes (FLN), and 2 ectoparasitic bacteria. EPN activity was also assessed by traditional insect-baiting, allowing the evaluation of FLN-EPN mixed progeny. EPNs were detected by qPCR in 50% of localities, and strongly correlated with EPN activity. Steinernema feltiae was the dominant EPN species measured by both techniques (qPCR and insect-bait), being widespread in all Algarve, while Heterorhabditis bacteriophora was detected mainly in citrus groves. The species S. arenarium and H. indica were detected by qPCR for the first time in continental Portugal. The molecular analysis of insect cadaver progeny revealed novel FLN-EPN associations with Pristionchus maupasi and P. pacificus. EPN, FLN and NF abundance differed among botanical groups, with citrus groves supporting high numbers of all trophic guilds. Oaks also favoured EPNs. Similarly, calcareous soil-ecoregion supported higher NF, FLN and EPN abundance. Two abiotic variables (pH, and clay content) explained the community variation in multivariate analysis, consistent with key abiotic variables described for other subtropical and temperate regions. The results supported the hypothesis that cultivated perennial habitats favour EPNs and soil organisms that can limit EPN activity as biological control agents. |
author2 |
Fundação para a Ciência e a Tecnologia (Portugal) |
author_facet |
Fundação para a Ciência e a Tecnologia (Portugal) Campos-Herrera, R. Blanco-Pérez, Rubén Bueno-Pallero, Francisco Ángel Duarte, Amílcar Nolasco, Gustavo Sommer, Ralf J. Rodríguez Martín, José Antonio |
format |
artículo |
topic_facet |
Entomopathogenic nematodes Habitat preference Mediterranean agro-ecosystems Multivariate analysis Quantitative real-time PCR |
author |
Campos-Herrera, R. Blanco-Pérez, Rubén Bueno-Pallero, Francisco Ángel Duarte, Amílcar Nolasco, Gustavo Sommer, Ralf J. Rodríguez Martín, José Antonio |
author_sort |
Campos-Herrera, R. |
title |
Vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: Evidence from the Algarve, Portugal |
title_short |
Vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: Evidence from the Algarve, Portugal |
title_full |
Vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: Evidence from the Algarve, Portugal |
title_fullStr |
Vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: Evidence from the Algarve, Portugal |
title_full_unstemmed |
Vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: Evidence from the Algarve, Portugal |
title_sort |
vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: evidence from the algarve, portugal |
publisher |
Elsevier |
publishDate |
2019-01 |
url |
http://hdl.handle.net/10261/193812 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100003329 |
work_keys_str_mv |
AT camposherrerar vegetationdrivesassemblagesofentomopathogenicnematodesandothersoilorganismsevidencefromthealgarveportugal AT blancoperezruben vegetationdrivesassemblagesofentomopathogenicnematodesandothersoilorganismsevidencefromthealgarveportugal AT buenopallerofranciscoangel vegetationdrivesassemblagesofentomopathogenicnematodesandothersoilorganismsevidencefromthealgarveportugal AT duarteamilcar vegetationdrivesassemblagesofentomopathogenicnematodesandothersoilorganismsevidencefromthealgarveportugal AT nolascogustavo vegetationdrivesassemblagesofentomopathogenicnematodesandothersoilorganismsevidencefromthealgarveportugal AT sommerralfj vegetationdrivesassemblagesofentomopathogenicnematodesandothersoilorganismsevidencefromthealgarveportugal AT rodriguezmartinjoseantonio vegetationdrivesassemblagesofentomopathogenicnematodesandothersoilorganismsevidencefromthealgarveportugal |
_version_ |
1777670957757890560 |
spelling |
dig-icvv-es-10261-1938122020-05-19T11:12:44Z Vegetation drives assemblages of entomopathogenic nematodes and other soil organisms: Evidence from the Algarve, Portugal Campos-Herrera, R. Blanco-Pérez, Rubén Bueno-Pallero, Francisco Ángel Duarte, Amílcar Nolasco, Gustavo Sommer, Ralf J. Rodríguez Martín, José Antonio Fundação para a Ciência e a Tecnologia (Portugal) Ministerio de Economía y Competitividad (España) Campos-Herrera, R. [0000-0003-0852-5269] Rodríguez Martín, José Antonio [0000-0002-9158-9564] Entomopathogenic nematodes Habitat preference Mediterranean agro-ecosystems Multivariate analysis Quantitative real-time PCR Entomopathogenic nematodes (EPNs) are widely distributed in soils throughout the world. Their activity as biological control agents is modulated by abiotic and biotic factors (e.g. soil type, climatic fluctuation and natural enemies). We sought to identify soil properties in a Mediterranean region, which might be managed to enhance biological control agents’ services provided by EPNs. We hypothesized that responses of EPN soil food web assemblages to abiotic factors in such a Mediterranean region would be consistent with previous observations in other biomes in subtropical and temperate regions, in which pH and variables related to water content were main drivers of such association. We also expected that EPN abundance and species composition would differ between stable botanical habitats (citrus groves, palmetto areas, oaks and pines), with EPNs and associated organisms favoured in cultivated sites (citrus). In spring 2016, 50 georeferenced localities, representing four botanical habitats and two soil-ecoregions (calcareous versus non-calcareous), were surveyed. Using published and de novo real time qPCR tools, we evaluated the frequency and abundance of 10 EPN species and 13 organisms associated with EPNs: 6 nematophagous fungi (NF), 5 free-living nematodes (FLN), and 2 ectoparasitic bacteria. EPN activity was also assessed by traditional insect-baiting, allowing the evaluation of FLN-EPN mixed progeny. EPNs were detected by qPCR in 50% of localities, and strongly correlated with EPN activity. Steinernema feltiae was the dominant EPN species measured by both techniques (qPCR and insect-bait), being widespread in all Algarve, while Heterorhabditis bacteriophora was detected mainly in citrus groves. The species S. arenarium and H. indica were detected by qPCR for the first time in continental Portugal. The molecular analysis of insect cadaver progeny revealed novel FLN-EPN associations with Pristionchus maupasi and P. pacificus. EPN, FLN and NF abundance differed among botanical groups, with citrus groves supporting high numbers of all trophic guilds. Oaks also favoured EPNs. Similarly, calcareous soil-ecoregion supported higher NF, FLN and EPN abundance. Two abiotic variables (pH, and clay content) explained the community variation in multivariate analysis, consistent with key abiotic variables described for other subtropical and temperate regions. The results supported the hypothesis that cultivated perennial habitats favour EPNs and soil organisms that can limit EPN activity as biological control agents. This work was supported by the Government of Portugal, thanks to the “Starting Grant” associate funds (grant number IF/00552/2014/CP1234/CT0007). RCH was awarded with an Investigator Programme contract (grant number IF/00552/2014), and currently is supported by the Ramón y Cajal Programme (Spanish Government, RYC-2016-19939). RBP and FBP were financed by the scientific assistantship fellowships associated to the grant IF/00552/2014/CP1234/CT0007 (two BI calls, UAlg-2016/004 and UAlg-2016/003, respectively). Peer reviewed 2019-10-30T12:11:55Z 2019-10-30T12:11:55Z 2019-01 artículo http://purl.org/coar/resource_type/c_6501 Soil Biology and Biochemistry 128: 150-163 (2019) 0038-0717 http://hdl.handle.net/10261/193812 10.1016/j.soilbio.2018.10.019 http://dx.doi.org/10.13039/501100001871 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2016-19939 http://dx.doi.org//10.1016/j.soilbio.2018.10.019 Sí none Elsevier |