Characterization and Pathogenicity of Botryosphaeriaceae species isolated from almond trees on the Island of Mallorca (Spain)

In this study, 31 almond orchards with trees showing severe decline symptoms were surveyed from 2009 to 2014 on the island of Mallorca (Spain). In all, 45 Botryosphaeriaceae isolates were collected and characterized based on phenotypical features and comparisons of DNA sequence data of the nuclear ribosomal DNA-internal transcribed spacer region and elongation factor 1-α gene. Five species were identified as Diplodia olivarum, D. seriata, Neofusicoccum luteum, N. mediterraneum, and N. parvum. Pathogenicity tests were performed on four cultivars (‘Pons’, ‘Vivot’, ‘Jordi’, and ‘Ferragnes’) under field conditions for two consecutive years (2013 to 2014), and confirmed that all five species cause canker and dieback of almond, with Neofusicoccum spp. more virulent than Diplodia spp. in both years. Jordi was less sensitive to fungal infection in 2013. First reports from almond in Spain include N. mediterraneum and N. luteum.

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Bibliographic Details
Main Authors: Olmo, Diego, Armengol, Josep, León, Maela, Gramaje, David
Other Authors: CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Format: artículo biblioteca
Language:English
Published: American Phytopathological Society 2016-12-30
Online Access:http://hdl.handle.net/10261/193392
http://dx.doi.org/10.13039/100007652
http://dx.doi.org/10.13039/501100000780
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spelling dig-icvv-es-10261-1933922019-10-26T01:22:53Z Characterization and Pathogenicity of Botryosphaeriaceae species isolated from almond trees on the Island of Mallorca (Spain) Olmo, Diego Armengol, Josep León, Maela Gramaje, David CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) European Commission Armengol, Josep [0000-0003-3815-8578] Gramaje, David [0000-0003-1755-3413] In this study, 31 almond orchards with trees showing severe decline symptoms were surveyed from 2009 to 2014 on the island of Mallorca (Spain). In all, 45 Botryosphaeriaceae isolates were collected and characterized based on phenotypical features and comparisons of DNA sequence data of the nuclear ribosomal DNA-internal transcribed spacer region and elongation factor 1-α gene. Five species were identified as Diplodia olivarum, D. seriata, Neofusicoccum luteum, N. mediterraneum, and N. parvum. Pathogenicity tests were performed on four cultivars (‘Pons’, ‘Vivot’, ‘Jordi’, and ‘Ferragnes’) under field conditions for two consecutive years (2013 to 2014), and confirmed that all five species cause canker and dieback of almond, with Neofusicoccum spp. more virulent than Diplodia spp. in both years. Jordi was less sensitive to fungal infection in 2013. First reports from almond in Spain include N. mediterraneum and N. luteum. D. Gramaje was supported by the DOC-INIA program from the National Institute for Agronomic Research (INIA), co-funded by the European Social Fund. We thank the researchers of ENOCIDA group for providing the incubators for the temperature growth assay and J. Moral for statistical advice. Peer reviewed 2019-10-25T07:57:30Z 2019-10-25T07:57:30Z 2016-12-30 artículo http://purl.org/coar/resource_type/c_6501 Plant Disease 100(2): 2483-2491 (2016) 0191-2917 http://hdl.handle.net/10261/193392 10.1094/PDIS-05-16-0676-RE 1943-7692 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 en http://dx.doi.org/10.1094/PDIS-05-16-0676-RE Sí none American Phytopathological Society
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country España
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language English
description In this study, 31 almond orchards with trees showing severe decline symptoms were surveyed from 2009 to 2014 on the island of Mallorca (Spain). In all, 45 Botryosphaeriaceae isolates were collected and characterized based on phenotypical features and comparisons of DNA sequence data of the nuclear ribosomal DNA-internal transcribed spacer region and elongation factor 1-α gene. Five species were identified as Diplodia olivarum, D. seriata, Neofusicoccum luteum, N. mediterraneum, and N. parvum. Pathogenicity tests were performed on four cultivars (‘Pons’, ‘Vivot’, ‘Jordi’, and ‘Ferragnes’) under field conditions for two consecutive years (2013 to 2014), and confirmed that all five species cause canker and dieback of almond, with Neofusicoccum spp. more virulent than Diplodia spp. in both years. Jordi was less sensitive to fungal infection in 2013. First reports from almond in Spain include N. mediterraneum and N. luteum.
author2 CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
author_facet CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Olmo, Diego
Armengol, Josep
León, Maela
Gramaje, David
format artículo
author Olmo, Diego
Armengol, Josep
León, Maela
Gramaje, David
spellingShingle Olmo, Diego
Armengol, Josep
León, Maela
Gramaje, David
Characterization and Pathogenicity of Botryosphaeriaceae species isolated from almond trees on the Island of Mallorca (Spain)
author_sort Olmo, Diego
title Characterization and Pathogenicity of Botryosphaeriaceae species isolated from almond trees on the Island of Mallorca (Spain)
title_short Characterization and Pathogenicity of Botryosphaeriaceae species isolated from almond trees on the Island of Mallorca (Spain)
title_full Characterization and Pathogenicity of Botryosphaeriaceae species isolated from almond trees on the Island of Mallorca (Spain)
title_fullStr Characterization and Pathogenicity of Botryosphaeriaceae species isolated from almond trees on the Island of Mallorca (Spain)
title_full_unstemmed Characterization and Pathogenicity of Botryosphaeriaceae species isolated from almond trees on the Island of Mallorca (Spain)
title_sort characterization and pathogenicity of botryosphaeriaceae species isolated from almond trees on the island of mallorca (spain)
publisher American Phytopathological Society
publishDate 2016-12-30
url http://hdl.handle.net/10261/193392
http://dx.doi.org/10.13039/100007652
http://dx.doi.org/10.13039/501100000780
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