Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties
[Aims] The study aimed to assess comparatively the accuracy and efficiency of three culture media protocols for estimating black-foot disease pathogens populations in soils and to examine how shifts in the abundance and composition of black-foot pathogens correspond to changes in specific soil properties. [Methods] Firstly, culture media were compared by evaluating the mycelial growth of selected black-foot pathogens and by estimating the population of Dactylonectria torresensis from artificially infested soils. Secondly, the most efficient culture medium was selected for estimating the viable propagules of black-foot disease pathogens in eight naturally infested soils. An analysis of the soil physicochemical properties was conducted. Data were statistically analyzed in order to explore possible relationships between the studied variables. [Results] Glucose-Faba Bean Rose Bengal Agar (GFBRBA) was selected as the most efficient culture medium. All naturally infested soils tested positive for the presence of black-foot pathogens. D. torresensis was the most frequently isolated species, followed by Dactylonectria alcacerensis and Ilyonectria liriodendri. A positive relationship between calcium carbonate and the Colony-Forming Units (CFUs) level of black-foot pathogens in soil was obtained. [Conclusions] In this study, we provide an early, specific, and accurate detection of viable propagules of black-foot pathogens in soil, which is critical to understand the ecology of these fungi and to design effective management strategie
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Springer Nature
2017-08
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Subjects: | Dactylonectria torresensis, Fungal ecology, Grapevine trunk disease, Soilborne inoculum, |
Online Access: | http://hdl.handle.net/10261/193388 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100007652 |
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dig-icvv-es-10261-1933882019-10-26T01:21:47Z Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties Berlanas, Carmen López-Manzanares, Beatriz Gramaje, David Gobierno de La Rioja CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) European Commission Berlanas, Carmen [0000-0003-1930-6272] López-Manzanares, Beatriz [0000-0003-0137-9297] Gramaje, David [0000-0003-1755-3413] Dactylonectria torresensis Fungal ecology Grapevine trunk disease Soilborne inoculum [Aims] The study aimed to assess comparatively the accuracy and efficiency of three culture media protocols for estimating black-foot disease pathogens populations in soils and to examine how shifts in the abundance and composition of black-foot pathogens correspond to changes in specific soil properties. [Methods] Firstly, culture media were compared by evaluating the mycelial growth of selected black-foot pathogens and by estimating the population of Dactylonectria torresensis from artificially infested soils. Secondly, the most efficient culture medium was selected for estimating the viable propagules of black-foot disease pathogens in eight naturally infested soils. An analysis of the soil physicochemical properties was conducted. Data were statistically analyzed in order to explore possible relationships between the studied variables. [Results] Glucose-Faba Bean Rose Bengal Agar (GFBRBA) was selected as the most efficient culture medium. All naturally infested soils tested positive for the presence of black-foot pathogens. D. torresensis was the most frequently isolated species, followed by Dactylonectria alcacerensis and Ilyonectria liriodendri. A positive relationship between calcium carbonate and the Colony-Forming Units (CFUs) level of black-foot pathogens in soil was obtained. [Conclusions] In this study, we provide an early, specific, and accurate detection of viable propagules of black-foot pathogens in soil, which is critical to understand the ecology of these fungi and to design effective management strategie The research was funded by CAR (Government of La Rioja, Spain), under the project “Characterization, epidemiology and control of fungal trunk pathogens of grapevine in La Rioja” (project number R-03-16). We thank “Instituto Navarro de Tecnologías e Infraestructuras Agroalimentarias” (INTIA), Spain, which collected the soil samples from the field in Olite for further use in the experiment. We thank P. Yécora and M. Andrés for technical assistance. David Gramaje was supported by the DOC-INIA program from the National Institute for Agronomic Research (INIA), co-funded by the European Social Fund. Carmen Berlanas was supported by the FPI-INIA program from the INIA. Beatriz López was supported by the PhD program from the Government of La Rioja. Peer reviewed 2019-10-25T07:40:21Z 2019-10-25T07:40:21Z 2017-08 artículo http://purl.org/coar/resource_type/c_6501 Plant and Soil 417(1-2): 467-479 (2017) 0032-079X http://hdl.handle.net/10261/193388 10.1007/s11104-017-3272-3 1573-5036 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100007652 en http://dx.doi.org/10.1007/s11104-017-3272-3 Sí none Springer Nature |
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Dactylonectria torresensis Fungal ecology Grapevine trunk disease Soilborne inoculum Dactylonectria torresensis Fungal ecology Grapevine trunk disease Soilborne inoculum |
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Dactylonectria torresensis Fungal ecology Grapevine trunk disease Soilborne inoculum Dactylonectria torresensis Fungal ecology Grapevine trunk disease Soilborne inoculum Berlanas, Carmen López-Manzanares, Beatriz Gramaje, David Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties |
description |
[Aims] The study aimed to assess comparatively the accuracy and efficiency of three culture media protocols for estimating black-foot disease pathogens populations in soils and to examine how shifts in the abundance and composition of black-foot pathogens correspond to changes in specific soil properties.
[Methods] Firstly, culture media were compared by evaluating the mycelial growth of selected black-foot pathogens and by estimating the population of Dactylonectria torresensis from artificially infested soils. Secondly, the most efficient culture medium was selected for estimating the viable propagules of black-foot disease pathogens in eight naturally infested soils. An analysis of the soil physicochemical properties was conducted. Data were statistically analyzed in order to explore possible relationships between the studied variables.
[Results] Glucose-Faba Bean Rose Bengal Agar (GFBRBA) was selected as the most efficient culture medium. All naturally infested soils tested positive for the presence of black-foot pathogens. D. torresensis was the most frequently isolated species, followed by Dactylonectria alcacerensis and Ilyonectria liriodendri. A positive relationship between calcium carbonate and the Colony-Forming Units (CFUs) level of black-foot pathogens in soil was obtained.
[Conclusions] In this study, we provide an early, specific, and accurate detection of viable propagules of black-foot pathogens in soil, which is critical to understand the ecology of these fungi and to design effective management strategie |
author2 |
Gobierno de La Rioja |
author_facet |
Gobierno de La Rioja Berlanas, Carmen López-Manzanares, Beatriz Gramaje, David |
format |
artículo |
topic_facet |
Dactylonectria torresensis Fungal ecology Grapevine trunk disease Soilborne inoculum |
author |
Berlanas, Carmen López-Manzanares, Beatriz Gramaje, David |
author_sort |
Berlanas, Carmen |
title |
Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties |
title_short |
Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties |
title_full |
Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties |
title_fullStr |
Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties |
title_full_unstemmed |
Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties |
title_sort |
estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties |
publisher |
Springer Nature |
publishDate |
2017-08 |
url |
http://hdl.handle.net/10261/193388 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100007652 |
work_keys_str_mv |
AT berlanascarmen estimationofviablepropagulesofblackfootdiseasepathogensingrapevinecultivatedsoilsandtheirrelationtoproductionsystemsandsoilproperties AT lopezmanzanaresbeatriz estimationofviablepropagulesofblackfootdiseasepathogensingrapevinecultivatedsoilsandtheirrelationtoproductionsystemsandsoilproperties AT gramajedavid estimationofviablepropagulesofblackfootdiseasepathogensingrapevinecultivatedsoilsandtheirrelationtoproductionsystemsandsoilproperties |
_version_ |
1777670953695707136 |