Antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (Parapenaeus longirostris)
Shrimp processing waste is the single largest industrial waste in Tunisia causing diverse environmental problems. Extraction of astaxanthin from the shell waste of the deep-water pink shrimp (Parapenaeus longirostris) was investigated. A hight astaxanthin yield was obtained using acetone (72.42 µg/g). Thin layer chromatographic separation of carotenoid extracts from P. longirostris yielded three distinct bands. The Rf values for the three bands were respectively 0.33, 0.60, 0.80 which corresponded to astaxanthin, astaxanthin monoester and astaxanthin diester. The antioxidant activities of astaxanthin at different concentrations were evaluated using various in vitro antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power, β-carotene bleaching and DNA nicking assays. Interestingly, astaxanthin extract showed strong antioxidant activity. The radical scavenging activities and reducing powers of astaxanthin were close to and even better than those of the synthetic antioxidant BHA. The astaxanthin extract from shrimp shell waste exhibited high inhibition of β-carotene bleaching and also showed a well DNA protection against degradation by hydroxyl radicals. The anti-proliferative activity of the astaxanthin extract was also investigated. The inhibition concentration (CI50) of proliferation of tumor cells of human laryngeal carcinoma (Hep 2 cells) was 300 µg astaxanthin /ml.
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Format: | artículo biblioteca |
Language: | English |
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Bentham Science Publishers
2013
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Subjects: | Parapenaeus longirostris, Astaxanthin, Antiproliferative activity, Antioxidant activity, Shrimp waste, |
Online Access: | http://hdl.handle.net/10261/88065 |
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dig-ictan-es-10261-880652021-06-17T10:31:17Z Antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (Parapenaeus longirostris) Sila, Assaâd Ayed-Ajmi, Y. Sayari, Nadhem Nasri, M. Martínez Álvarez, Óscar Bougatef, Ali Parapenaeus longirostris Astaxanthin Antiproliferative activity Antioxidant activity Shrimp waste Shrimp processing waste is the single largest industrial waste in Tunisia causing diverse environmental problems. Extraction of astaxanthin from the shell waste of the deep-water pink shrimp (Parapenaeus longirostris) was investigated. A hight astaxanthin yield was obtained using acetone (72.42 µg/g). Thin layer chromatographic separation of carotenoid extracts from P. longirostris yielded three distinct bands. The Rf values for the three bands were respectively 0.33, 0.60, 0.80 which corresponded to astaxanthin, astaxanthin monoester and astaxanthin diester. The antioxidant activities of astaxanthin at different concentrations were evaluated using various in vitro antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power, β-carotene bleaching and DNA nicking assays. Interestingly, astaxanthin extract showed strong antioxidant activity. The radical scavenging activities and reducing powers of astaxanthin were close to and even better than those of the synthetic antioxidant BHA. The astaxanthin extract from shrimp shell waste exhibited high inhibition of β-carotene bleaching and also showed a well DNA protection against degradation by hydroxyl radicals. The anti-proliferative activity of the astaxanthin extract was also investigated. The inhibition concentration (CI50) of proliferation of tumor cells of human laryngeal carcinoma (Hep 2 cells) was 300 µg astaxanthin /ml. Peer Reviewed 2013-12-03T09:58:25Z 2013-12-03T09:58:25Z 2013 2013-12-03T09:58:25Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.2174/2210315511303020002 issn: 2210-3155 The Natural Products Journal 3: 82-89 (2013) http://hdl.handle.net/10261/88065 10.2174/2210315511303020002 en none Bentham Science Publishers |
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Parapenaeus longirostris Astaxanthin Antiproliferative activity Antioxidant activity Shrimp waste Parapenaeus longirostris Astaxanthin Antiproliferative activity Antioxidant activity Shrimp waste |
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Parapenaeus longirostris Astaxanthin Antiproliferative activity Antioxidant activity Shrimp waste Parapenaeus longirostris Astaxanthin Antiproliferative activity Antioxidant activity Shrimp waste Sila, Assaâd Ayed-Ajmi, Y. Sayari, Nadhem Nasri, M. Martínez Álvarez, Óscar Bougatef, Ali Antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (Parapenaeus longirostris) |
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Shrimp processing waste is the single largest industrial waste in Tunisia causing diverse environmental problems. Extraction of astaxanthin from the shell waste of the deep-water pink shrimp (Parapenaeus longirostris) was investigated. A hight astaxanthin yield was obtained using acetone (72.42 µg/g). Thin layer chromatographic separation of carotenoid extracts from P. longirostris yielded three distinct bands. The Rf values for the three bands were respectively 0.33, 0.60, 0.80 which corresponded to astaxanthin, astaxanthin monoester and astaxanthin diester. The antioxidant activities of astaxanthin at different concentrations were evaluated using various in vitro antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power, β-carotene bleaching and DNA nicking assays. Interestingly, astaxanthin extract showed strong antioxidant activity. The radical scavenging activities and reducing powers of astaxanthin were close to and even better than those of the synthetic antioxidant BHA. The astaxanthin extract from shrimp shell waste exhibited high inhibition of β-carotene bleaching and also showed a well DNA protection against degradation by hydroxyl radicals. The anti-proliferative activity of the astaxanthin extract was also investigated. The inhibition concentration (CI50) of proliferation of tumor cells of human laryngeal carcinoma (Hep 2 cells) was 300 µg astaxanthin /ml. |
format |
artículo |
topic_facet |
Parapenaeus longirostris Astaxanthin Antiproliferative activity Antioxidant activity Shrimp waste |
author |
Sila, Assaâd Ayed-Ajmi, Y. Sayari, Nadhem Nasri, M. Martínez Álvarez, Óscar Bougatef, Ali |
author_facet |
Sila, Assaâd Ayed-Ajmi, Y. Sayari, Nadhem Nasri, M. Martínez Álvarez, Óscar Bougatef, Ali |
author_sort |
Sila, Assaâd |
title |
Antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (Parapenaeus longirostris) |
title_short |
Antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (Parapenaeus longirostris) |
title_full |
Antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (Parapenaeus longirostris) |
title_fullStr |
Antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (Parapenaeus longirostris) |
title_full_unstemmed |
Antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (Parapenaeus longirostris) |
title_sort |
antioxidant and anti-proliferative activities of astaxanthin extracted from the shell waste of deep-water pink shrimp (parapenaeus longirostris) |
publisher |
Bentham Science Publishers |
publishDate |
2013 |
url |
http://hdl.handle.net/10261/88065 |
work_keys_str_mv |
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