Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes
This study presents an approach that utilizes low-value agro-industrial by-products as culture media for producing high-value proteolytic enzymes. The objective was to assess the impact of six agro-industrial by-products as culture media on the production of proteolytic enzymes. Bacillus subtilis strains, confirmed through comprehensive biochemical, morphological, and molecular analyses, were isolated and identified. Enzymatic activity was evaluated using azocasein and casein substrates, and the molecular sizes of the purified extract components were determined. The results demonstrated that the isolated bacteria exhibited higher metabolic and enzymatic activity when cultured in media containing 1 % soybean oil cake or feather meal. Furthermore, higher concentrations of the culture media were found to hinder the production of protease. Optimal protease synthesis on soybean oil cake and feather meal media was achieved after 4 days, using both the azocasein and casein methods. Semi-purification of the enzymatic extract obtained from Bacillus subtilis in feather meal and soybean oil cake resulted in a significant increase in azocaseinolytic and caseinolytic activities. Gel electrophoresis analysis revealed multiple bands in the fractions with the highest enzymatic activity in soybean oil cake, indicating the presence of various enzymes with varying molecular sizes. These findings highlight the potential of utilizing low-value agro-industrial by-products as efficient culture media for the sustainable and economically viable production of proteolytic enzymes with promising applications in various industries.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2023
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Subjects: | Proteolytic enzymes, Agro-industrial byproducts, Bacillus subtilis, Enzymatic activity, Culture media, Sustainable production, Purification, |
Online Access: | http://hdl.handle.net/10261/345241 |
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dig-ictan-es-10261-3452412024-02-05T07:20:14Z Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes Sisa, Alisson Sotomayor, Cristina Buitrón, Lucía Gómez Estaca, Joaquín Martínez Álvarez, Óscar Mosquera, Mauricio Consejo Superior de Investigaciones Científicas (España) Escuela Politécnica Nacional (Ecuador) Proteolytic enzymes Agro-industrial byproducts Bacillus subtilis Enzymatic activity Culture media Sustainable production Purification This study presents an approach that utilizes low-value agro-industrial by-products as culture media for producing high-value proteolytic enzymes. The objective was to assess the impact of six agro-industrial by-products as culture media on the production of proteolytic enzymes. Bacillus subtilis strains, confirmed through comprehensive biochemical, morphological, and molecular analyses, were isolated and identified. Enzymatic activity was evaluated using azocasein and casein substrates, and the molecular sizes of the purified extract components were determined. The results demonstrated that the isolated bacteria exhibited higher metabolic and enzymatic activity when cultured in media containing 1 % soybean oil cake or feather meal. Furthermore, higher concentrations of the culture media were found to hinder the production of protease. Optimal protease synthesis on soybean oil cake and feather meal media was achieved after 4 days, using both the azocasein and casein methods. Semi-purification of the enzymatic extract obtained from Bacillus subtilis in feather meal and soybean oil cake resulted in a significant increase in azocaseinolytic and caseinolytic activities. Gel electrophoresis analysis revealed multiple bands in the fractions with the highest enzymatic activity in soybean oil cake, indicating the presence of various enzymes with varying molecular sizes. These findings highlight the potential of utilizing low-value agro-industrial by-products as efficient culture media for the sustainable and economically viable production of proteolytic enzymes with promising applications in various industries. Dr. Mauricio Esteban Mosquera was supported by Escuela Politécnica Nacional {PIJ 16–12}. Oscar Martinez was suported by the Spanish National Research Council (CSIC, Spain) under project iCOOPA22013. Peer reviewed 2024-02-05T07:20:14Z 2024-02-05T07:20:14Z 2023 artículo Heliyon 9(10): e20735 (2023) http://hdl.handle.net/10261/345241 10.1016/j.heliyon.2023.e20735 2405-8440 37867804 en Publisher's version The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.heliyon.2023.e20735 https://doi.org/10.1016/j.heliyon.2023.e20735 Sí open application/pdf Elsevier |
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Proteolytic enzymes Agro-industrial byproducts Bacillus subtilis Enzymatic activity Culture media Sustainable production Purification Proteolytic enzymes Agro-industrial byproducts Bacillus subtilis Enzymatic activity Culture media Sustainable production Purification |
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Proteolytic enzymes Agro-industrial byproducts Bacillus subtilis Enzymatic activity Culture media Sustainable production Purification Proteolytic enzymes Agro-industrial byproducts Bacillus subtilis Enzymatic activity Culture media Sustainable production Purification Sisa, Alisson Sotomayor, Cristina Buitrón, Lucía Gómez Estaca, Joaquín Martínez Álvarez, Óscar Mosquera, Mauricio Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes |
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This study presents an approach that utilizes low-value agro-industrial by-products as culture media for producing high-value proteolytic enzymes. The objective was to assess the impact of six agro-industrial by-products as culture media on the production of proteolytic enzymes. Bacillus subtilis strains, confirmed through comprehensive biochemical, morphological, and molecular analyses, were isolated and identified. Enzymatic activity was evaluated using azocasein and casein substrates, and the molecular sizes of the purified extract components were determined. The results demonstrated that the isolated bacteria exhibited higher metabolic and enzymatic activity when cultured in media containing 1 % soybean oil cake or feather meal. Furthermore, higher concentrations of the culture media were found to hinder the production of protease. Optimal protease synthesis on soybean oil cake and feather meal media was achieved after 4 days, using both the azocasein and casein methods. Semi-purification of the enzymatic extract obtained from Bacillus subtilis in feather meal and soybean oil cake resulted in a significant increase in azocaseinolytic and caseinolytic activities. Gel electrophoresis analysis revealed multiple bands in the fractions with the highest enzymatic activity in soybean oil cake, indicating the presence of various enzymes with varying molecular sizes. These findings highlight the potential of utilizing low-value agro-industrial by-products as efficient culture media for the sustainable and economically viable production of proteolytic enzymes with promising applications in various industries. |
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Consejo Superior de Investigaciones Científicas (España) |
author_facet |
Consejo Superior de Investigaciones Científicas (España) Sisa, Alisson Sotomayor, Cristina Buitrón, Lucía Gómez Estaca, Joaquín Martínez Álvarez, Óscar Mosquera, Mauricio |
format |
artículo |
topic_facet |
Proteolytic enzymes Agro-industrial byproducts Bacillus subtilis Enzymatic activity Culture media Sustainable production Purification |
author |
Sisa, Alisson Sotomayor, Cristina Buitrón, Lucía Gómez Estaca, Joaquín Martínez Álvarez, Óscar Mosquera, Mauricio |
author_sort |
Sisa, Alisson |
title |
Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes |
title_short |
Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes |
title_full |
Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes |
title_fullStr |
Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes |
title_full_unstemmed |
Evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes |
title_sort |
evaluation of by-products from agricultural, livestock and fishing industries as nutrient source for the production of proteolytic enzymes |
publisher |
Elsevier |
publishDate |
2023 |
url |
http://hdl.handle.net/10261/345241 |
work_keys_str_mv |
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