Dual 6Pβ-Galactosidase/6Pβ-Glucosidase GH1 Family for Lactose Metabolism in the Probiotic Bacterium Lactiplantibacillus plantarum WCFS1
Intestinal lactic acid bacteria can help alleviate lactose maldigestion by promoting lactose hydrolysis in the small intestine. This study shows that protein extracts from probiotic bacterium Lactiplantibacillus plantarum WCFS1 possess two metabolic pathways for lactose metabolism, involving β-galactosidase (β-gal) and 6Pβ-galactosidase (6Pβ-gal) activities. As L. plantarum WCFS1 genome lacks a putative 6Pβ-gal gene, the 11 GH1 family proteins, in which their 6Pβ-glucosidase (6Pβ-glc) activity was experimentally demonstrated,, were assayed for 6Pβ-gal activity. Among them, only Lp_3525 (Pbg9) also exhibited a high 6Pβ-gal activity. The sequence comparison of this dual 6Pβ-gal/6Pβ-glc GH1 protein to previously described dual GH1 proteins revealed that L. plantarum WCFS1 Lp_3525 belonged to a new group of dual 6Pβ-gal/6Pβ-glc GH1 proteins, as it possessed conserved residues and structural motifs mainly present in 6Pβ-glc GH1 proteins. Finally, Lp_3525 exhibited, under intestinal conditions, an adequate 6Pβ-gal activity with possible relevance for lactose maldigestion management.
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Language: | English |
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American Chemical Society
2023-07-06
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Subjects: | Lactose maldigestion, Lactose intolerance, Glycoside hydrolase, GH1, Dual phospho glycosidase, |
Online Access: | http://hdl.handle.net/10261/331786 |
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dig-ictan-es-10261-3317862023-07-25T09:04:12Z Dual 6Pβ-Galactosidase/6Pβ-Glucosidase GH1 Family for Lactose Metabolism in the Probiotic Bacterium Lactiplantibacillus plantarum WCFS1 Plaza-Vinuesa, Laura Sánchez-Arroyo, Ana Moreno, F. Javier Rivas, Blanca de las Muñoz, Rosario Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) European Commission Lactose maldigestion Lactose intolerance Glycoside hydrolase GH1 Dual phospho glycosidase Intestinal lactic acid bacteria can help alleviate lactose maldigestion by promoting lactose hydrolysis in the small intestine. This study shows that protein extracts from probiotic bacterium Lactiplantibacillus plantarum WCFS1 possess two metabolic pathways for lactose metabolism, involving β-galactosidase (β-gal) and 6Pβ-galactosidase (6Pβ-gal) activities. As L. plantarum WCFS1 genome lacks a putative 6Pβ-gal gene, the 11 GH1 family proteins, in which their 6Pβ-glucosidase (6Pβ-glc) activity was experimentally demonstrated,, were assayed for 6Pβ-gal activity. Among them, only Lp_3525 (Pbg9) also exhibited a high 6Pβ-gal activity. The sequence comparison of this dual 6Pβ-gal/6Pβ-glc GH1 protein to previously described dual GH1 proteins revealed that L. plantarum WCFS1 Lp_3525 belonged to a new group of dual 6Pβ-gal/6Pβ-glc GH1 proteins, as it possessed conserved residues and structural motifs mainly present in 6Pβ-glc GH1 proteins. Finally, Lp_3525 exhibited, under intestinal conditions, an adequate 6Pβ-gal activity with possible relevance for lactose maldigestion management. This work was financially supported by grants AGL2017-84614-C2-1-R and AGL2017-84614-C2-2-R funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe. Ana Sánchez Arroyo is a recipient of the PRE2018-083862 FPI contract funded by MCIN/AEI/10.13039/501100011033 and by ESF Investing in your future. Peer reviewed 2023-07-25T09:04:12Z 2023-07-25T09:04:12Z 2023-07-06 artículo Journal of Agricultural and Food Chemistry 71(28): 10693-10700 (2023) 0021-8561 http://hdl.handle.net/10261/331786 10.1021/acs.jafc.3c01158 1520-5118 37409693 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84614-C2-1-R/ES/NUEVAS ESTRATEGIAS PARA LA SINTESIS DE COMPUESTOS BIOACTIVOS DE INTERES ALIMENTARIO EMPLEANDO ENZIMAS MODIFICADORAS DE CARBOHIDRATOS DERIVADAS DE LACTOBACILLUS PLANTARUM WCFS1/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-84614-C2-2-R/ES/ENZIMAS MODIFICADORAS DE CARBOHIDRATOS DE LACTOBACILLUS PLANTARUM WCFS1: CARACTERIZACION GENETICA, FUNCIONAL Y ESTRUCTURAL/ info:eu-repo/grantAgreement/AEI//PRE2018-083862 Publisher's version https://doi.org/10.1021/acs.jafc.3c01158 Sí open application/pdf American Chemical Society |
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Lactose maldigestion Lactose intolerance Glycoside hydrolase GH1 Dual phospho glycosidase Lactose maldigestion Lactose intolerance Glycoside hydrolase GH1 Dual phospho glycosidase |
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Lactose maldigestion Lactose intolerance Glycoside hydrolase GH1 Dual phospho glycosidase Lactose maldigestion Lactose intolerance Glycoside hydrolase GH1 Dual phospho glycosidase Plaza-Vinuesa, Laura Sánchez-Arroyo, Ana Moreno, F. Javier Rivas, Blanca de las Muñoz, Rosario Dual 6Pβ-Galactosidase/6Pβ-Glucosidase GH1 Family for Lactose Metabolism in the Probiotic Bacterium Lactiplantibacillus plantarum WCFS1 |
description |
Intestinal lactic acid bacteria can help alleviate lactose maldigestion by promoting lactose hydrolysis in the small intestine. This study shows that protein extracts from probiotic bacterium Lactiplantibacillus plantarum WCFS1 possess two metabolic pathways for lactose metabolism, involving β-galactosidase (β-gal) and 6Pβ-galactosidase (6Pβ-gal) activities. As L. plantarum WCFS1 genome lacks a putative 6Pβ-gal gene, the 11 GH1 family proteins, in which their 6Pβ-glucosidase (6Pβ-glc) activity was experimentally demonstrated,, were assayed for 6Pβ-gal activity. Among them, only Lp_3525 (Pbg9) also exhibited a high 6Pβ-gal activity. The sequence comparison of this dual 6Pβ-gal/6Pβ-glc GH1 protein to previously described dual GH1 proteins revealed that L. plantarum WCFS1 Lp_3525 belonged to a new group of dual 6Pβ-gal/6Pβ-glc GH1 proteins, as it possessed conserved residues and structural motifs mainly present in 6Pβ-glc GH1 proteins. Finally, Lp_3525 exhibited, under intestinal conditions, an adequate 6Pβ-gal activity with possible relevance for lactose maldigestion management. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Plaza-Vinuesa, Laura Sánchez-Arroyo, Ana Moreno, F. Javier Rivas, Blanca de las Muñoz, Rosario |
format |
artículo |
topic_facet |
Lactose maldigestion Lactose intolerance Glycoside hydrolase GH1 Dual phospho glycosidase |
author |
Plaza-Vinuesa, Laura Sánchez-Arroyo, Ana Moreno, F. Javier Rivas, Blanca de las Muñoz, Rosario |
author_sort |
Plaza-Vinuesa, Laura |
title |
Dual 6Pβ-Galactosidase/6Pβ-Glucosidase GH1 Family for Lactose Metabolism in the Probiotic Bacterium Lactiplantibacillus plantarum WCFS1 |
title_short |
Dual 6Pβ-Galactosidase/6Pβ-Glucosidase GH1 Family for Lactose Metabolism in the Probiotic Bacterium Lactiplantibacillus plantarum WCFS1 |
title_full |
Dual 6Pβ-Galactosidase/6Pβ-Glucosidase GH1 Family for Lactose Metabolism in the Probiotic Bacterium Lactiplantibacillus plantarum WCFS1 |
title_fullStr |
Dual 6Pβ-Galactosidase/6Pβ-Glucosidase GH1 Family for Lactose Metabolism in the Probiotic Bacterium Lactiplantibacillus plantarum WCFS1 |
title_full_unstemmed |
Dual 6Pβ-Galactosidase/6Pβ-Glucosidase GH1 Family for Lactose Metabolism in the Probiotic Bacterium Lactiplantibacillus plantarum WCFS1 |
title_sort |
dual 6pβ-galactosidase/6pβ-glucosidase gh1 family for lactose metabolism in the probiotic bacterium lactiplantibacillus plantarum wcfs1 |
publisher |
American Chemical Society |
publishDate |
2023-07-06 |
url |
http://hdl.handle.net/10261/331786 |
work_keys_str_mv |
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