Effect of growth factors on the in vitro growth and differentiation of early and late passage C6 glioma cells
The effect of different hormones and growth factors was assayed on the in vitro growth and enzymatic activities of 2',Y-cyclic nucleotide Yphosphohydrolase (CNP) and glutamine synthetase (GS) of rat glioma C6 cells at two different passages in culture. Young cultures (passage 26), mainly oligodendrocytic, and older cultures (passage 134), predominantly astrocytic, were treated with 10 #M dexamethasone, 20ng/ml transforming growth factor alpha (TGF~), 10ng/ml insulin, 20 ng/ml platelet-derived growth factor (PDGF), and 20 ng/ml, epidermal growth factor (EGF) in serum-free chemically defined media. In vitro growth rate was measured in terms of DNA content, by a fluorometric method of diaminobenzoic acid, and rate of DNA synthesis by 3H-thymidine incorporation. CNP activity (marker for in vitro oligodendrocytes) and GS activity (marker for astrocytes) were determined spectrophotometrically. Dexamethasone reversibly and significantly inhibited growth of C6 glioma in early and late passages. PDGF and insulin promoted in vitro growth only in late passage but not in early passage cells, whereas EGF and TGFct did not significantly affect growth. An increase in CNP activity was observed in early passage cells under the effect of PDGF and insulin. The increase in GS activity induced by insulin and dexamethasone suggests a differentiating role for these factors in C6 glioma cells. These results further present the C6 glioma cell line as a useful model for studies on glial cell properties and responsiveness in culture and support its use in experimental aging in vitro.
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1996
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dig-ictan-es-10261-2416002021-05-28T02:51:07Z Effect of growth factors on the in vitro growth and differentiation of early and late passage C6 glioma cells Goya, Luis Feng, Pei-Ti Aliabadi, Samira Timiras, Paola S. The effect of different hormones and growth factors was assayed on the in vitro growth and enzymatic activities of 2',Y-cyclic nucleotide Yphosphohydrolase (CNP) and glutamine synthetase (GS) of rat glioma C6 cells at two different passages in culture. Young cultures (passage 26), mainly oligodendrocytic, and older cultures (passage 134), predominantly astrocytic, were treated with 10 #M dexamethasone, 20ng/ml transforming growth factor alpha (TGF~), 10ng/ml insulin, 20 ng/ml platelet-derived growth factor (PDGF), and 20 ng/ml, epidermal growth factor (EGF) in serum-free chemically defined media. In vitro growth rate was measured in terms of DNA content, by a fluorometric method of diaminobenzoic acid, and rate of DNA synthesis by 3H-thymidine incorporation. CNP activity (marker for in vitro oligodendrocytes) and GS activity (marker for astrocytes) were determined spectrophotometrically. Dexamethasone reversibly and significantly inhibited growth of C6 glioma in early and late passages. PDGF and insulin promoted in vitro growth only in late passage but not in early passage cells, whereas EGF and TGFct did not significantly affect growth. An increase in CNP activity was observed in early passage cells under the effect of PDGF and insulin. The increase in GS activity induced by insulin and dexamethasone suggests a differentiating role for these factors in C6 glioma cells. These results further present the C6 glioma cell line as a useful model for studies on glial cell properties and responsiveness in culture and support its use in experimental aging in vitro. Peer reviewed 2021-05-25T12:00:45Z 2021-05-25T12:00:45Z 1996 artículo http://purl.org/coar/resource_type/c_6501 International Journal of Developmental Neuroscience 14(4): 409-417 (1996) 0736-5748 http://hdl.handle.net/10261/241600 en Sí none Elsevier |
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The effect of different hormones and growth factors was assayed on the in vitro growth and enzymatic activities of 2',Y-cyclic nucleotide Yphosphohydrolase (CNP) and glutamine synthetase (GS) of rat glioma C6 cells at two different passages in culture. Young cultures (passage 26), mainly oligodendrocytic, and older cultures (passage 134), predominantly astrocytic, were treated with 10 #M dexamethasone, 20ng/ml transforming growth factor alpha (TGF~), 10ng/ml insulin, 20 ng/ml platelet-derived growth factor (PDGF), and 20 ng/ml, epidermal growth factor (EGF) in serum-free chemically defined media. In vitro growth rate was measured in terms of DNA content, by a fluorometric method of diaminobenzoic acid, and rate of DNA synthesis by 3H-thymidine incorporation. CNP activity (marker for in vitro oligodendrocytes) and GS activity (marker for astrocytes) were determined spectrophotometrically. Dexamethasone reversibly and significantly inhibited growth of C6 glioma in early and late passages. PDGF and insulin promoted in vitro growth only in late passage but not in early passage cells, whereas EGF and TGFct did not significantly affect growth. An increase in CNP activity was observed in early passage cells under the effect of PDGF and insulin. The increase in GS activity induced by insulin and dexamethasone suggests a differentiating role for these factors in C6 glioma cells. These results further present the C6 glioma cell line as a useful model for studies on glial cell properties and responsiveness in culture and support its use in experimental aging in vitro. |
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artículo |
author |
Goya, Luis Feng, Pei-Ti Aliabadi, Samira Timiras, Paola S. |
spellingShingle |
Goya, Luis Feng, Pei-Ti Aliabadi, Samira Timiras, Paola S. Effect of growth factors on the in vitro growth and differentiation of early and late passage C6 glioma cells |
author_facet |
Goya, Luis Feng, Pei-Ti Aliabadi, Samira Timiras, Paola S. |
author_sort |
Goya, Luis |
title |
Effect of growth factors on the in vitro growth and differentiation of early and late passage C6 glioma cells |
title_short |
Effect of growth factors on the in vitro growth and differentiation of early and late passage C6 glioma cells |
title_full |
Effect of growth factors on the in vitro growth and differentiation of early and late passage C6 glioma cells |
title_fullStr |
Effect of growth factors on the in vitro growth and differentiation of early and late passage C6 glioma cells |
title_full_unstemmed |
Effect of growth factors on the in vitro growth and differentiation of early and late passage C6 glioma cells |
title_sort |
effect of growth factors on the in vitro growth and differentiation of early and late passage c6 glioma cells |
publisher |
Elsevier |
publishDate |
1996 |
url |
http://hdl.handle.net/10261/241600 |
work_keys_str_mv |
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_version_ |
1777670703954264064 |