An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene
The isocratic separation of 14 carotenoids, as well as retinol, retinyl acetate, retinyl palmitate, α-tocopherol and tocopherol acetate, is accomplished in 12 minutes, using a Spheri-5-ODS column and acetonitrile:dichloromethane:methanol (70:20:10) as mobile phase, with two-channel, programmable multiwavelength detection. The carotenoids separated are as follows: lutein/zeaxanthin, canthaxanthin, β-apo-8′ carotenal, β-cryptoxanthin, echinenone, lycopene, γ-carotene, α-carotene, β-carotene, 9-cis-β-carotene, 15-cis-/13-cis-β-carotene, phytoene and phytofluene. The separation of lutein and zeaxanthin is obtained simply by changing the mobile phase to acetonitrile:methanol (85:15).
Saved in:
Main Authors: | Granado Lorencio, Fernando, Olmedilla Alonso, Begoña, Blanco, Inmaculada, Rojas-Hidalgo, E. |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Taylor & Francis
1991
|
Online Access: | http://hdl.handle.net/10261/241028 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Assessment of food sources and the intake of the colourless carotenoids phytoene and phytofluene in Spain
by: Olmedilla Alonso, Begoña, et al.
Published: (2021-12-11) -
Status and dietary intake of phytoene and phytofluene in Spanish adults and the effect of a four-week dietary intervention with lutein-rich fruits or vegetables
by: Rodríguez-Rodríguez, Elena, et al.
Published: (2022) -
Influence of different cooking methods in total carotenoids contents, a- and B-carotene and cis-B-carotene isomers in pumpkin (Cucurbita moschata Duch.).
by: RIBEIRO, E. M. G., et al.
Published: (2012-10-16) -
Impact of thermal treatments on the bioaccessibility of phytoene and phytofluene in relation to changes in the microstructure and size of orange juice particles
by: Mapelli-Brahm, Paula, et al.
Published: (2018-04-26) -
A rapid separation of ten carotenoids, three retinoids, alpha-tocopherol and d-alpha-tocopherol acetate by high performance liquid chromatography and its application to serum and vegetable samples
by: Olmedilla Alonso, Begoña, et al.
Published: (1990)