An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene

An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene

The isocratic separation of 14 carotenoids, as well as retinol, retinyl acetate, retinyl palmitate, α-tocopherol and tocopherol acetate, is accomplished in 12 minutes, using a Spheri-5-ODS column and acetonitrile:dichloromethane:methanol (70:20:10) as mobile phase, with two-channel, programmable multiwavelength detection. The carotenoids separated are as follows: lutein/zeaxanthin, canthaxanthin, β-apo-8′ carotenal, β-cryptoxanthin, echinenone, lycopene, γ-carotene, α-carotene, β-carotene, 9-cis-β-carotene, 15-cis-/13-cis-β-carotene, phytoene and phytofluene. The separation of lutein and zeaxanthin is obtained simply by changing the mobile phase to acetonitrile:methanol (85:15).

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Bibliographic Details
Main Authors: Granado Lorencio, Fernando, Olmedilla Alonso, Begoña, Blanco, Inmaculada, Rojas-Hidalgo, E.
Format: artículo biblioteca
Language:English
Published: Taylor & Francis 1991
Online Access:http://hdl.handle.net/10261/241028
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spelling dig-ictan-es-10261-2410282022-07-12T10:09:20Z An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene Granado Lorencio, Fernando Olmedilla Alonso, Begoña Blanco, Inmaculada Rojas-Hidalgo, E. The isocratic separation of 14 carotenoids, as well as retinol, retinyl acetate, retinyl palmitate, α-tocopherol and tocopherol acetate, is accomplished in 12 minutes, using a Spheri-5-ODS column and acetonitrile:dichloromethane:methanol (70:20:10) as mobile phase, with two-channel, programmable multiwavelength detection. The carotenoids separated are as follows: lutein/zeaxanthin, canthaxanthin, β-apo-8′ carotenal, β-cryptoxanthin, echinenone, lycopene, γ-carotene, α-carotene, β-carotene, 9-cis-β-carotene, 15-cis-/13-cis-β-carotene, phytoene and phytofluene. The separation of lutein and zeaxanthin is obtained simply by changing the mobile phase to acetonitrile:methanol (85:15). Peer reviewed 2021-05-18T08:41:48Z 2021-05-18T08:41:48Z 1991 artículo http://purl.org/coar/resource_type/c_6501 Journal of Liquid Chromatography 14(13): 2457-2475 (1991) 0148-3919 http://hdl.handle.net/10261/241028 10.1080/01483919108049333 en https://doi.org/10.1080/01483919108049333 No none Taylor & Francis
institution ICTAN ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ictan-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICTAN España
language English
description The isocratic separation of 14 carotenoids, as well as retinol, retinyl acetate, retinyl palmitate, α-tocopherol and tocopherol acetate, is accomplished in 12 minutes, using a Spheri-5-ODS column and acetonitrile:dichloromethane:methanol (70:20:10) as mobile phase, with two-channel, programmable multiwavelength detection. The carotenoids separated are as follows: lutein/zeaxanthin, canthaxanthin, β-apo-8′ carotenal, β-cryptoxanthin, echinenone, lycopene, γ-carotene, α-carotene, β-carotene, 9-cis-β-carotene, 15-cis-/13-cis-β-carotene, phytoene and phytofluene. The separation of lutein and zeaxanthin is obtained simply by changing the mobile phase to acetonitrile:methanol (85:15).
format artículo
author Granado Lorencio, Fernando
Olmedilla Alonso, Begoña
Blanco, Inmaculada
Rojas-Hidalgo, E.
spellingShingle Granado Lorencio, Fernando
Olmedilla Alonso, Begoña
Blanco, Inmaculada
Rojas-Hidalgo, E.
An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene
author_facet Granado Lorencio, Fernando
Olmedilla Alonso, Begoña
Blanco, Inmaculada
Rojas-Hidalgo, E.
author_sort Granado Lorencio, Fernando
title An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene
title_short An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene
title_full An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene
title_fullStr An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene
title_full_unstemmed An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene
title_sort improved hplc method for the separation of fourteen carotenoids, including 15-/13- and 9-cis-β-carotene isomers, phytoene and phytofluene
publisher Taylor & Francis
publishDate 1991
url http://hdl.handle.net/10261/241028
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