An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene
The isocratic separation of 14 carotenoids, as well as retinol, retinyl acetate, retinyl palmitate, α-tocopherol and tocopherol acetate, is accomplished in 12 minutes, using a Spheri-5-ODS column and acetonitrile:dichloromethane:methanol (70:20:10) as mobile phase, with two-channel, programmable multiwavelength detection. The carotenoids separated are as follows: lutein/zeaxanthin, canthaxanthin, β-apo-8′ carotenal, β-cryptoxanthin, echinenone, lycopene, γ-carotene, α-carotene, β-carotene, 9-cis-β-carotene, 15-cis-/13-cis-β-carotene, phytoene and phytofluene. The separation of lutein and zeaxanthin is obtained simply by changing the mobile phase to acetonitrile:methanol (85:15).
Main Authors: | , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Taylor & Francis
1991
|
Online Access: | http://hdl.handle.net/10261/241028 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-ictan-es-10261-241028 |
---|---|
record_format |
koha |
spelling |
dig-ictan-es-10261-2410282022-07-12T10:09:20Z An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene Granado Lorencio, Fernando Olmedilla Alonso, Begoña Blanco, Inmaculada Rojas-Hidalgo, E. The isocratic separation of 14 carotenoids, as well as retinol, retinyl acetate, retinyl palmitate, α-tocopherol and tocopherol acetate, is accomplished in 12 minutes, using a Spheri-5-ODS column and acetonitrile:dichloromethane:methanol (70:20:10) as mobile phase, with two-channel, programmable multiwavelength detection. The carotenoids separated are as follows: lutein/zeaxanthin, canthaxanthin, β-apo-8′ carotenal, β-cryptoxanthin, echinenone, lycopene, γ-carotene, α-carotene, β-carotene, 9-cis-β-carotene, 15-cis-/13-cis-β-carotene, phytoene and phytofluene. The separation of lutein and zeaxanthin is obtained simply by changing the mobile phase to acetonitrile:methanol (85:15). Peer reviewed 2021-05-18T08:41:48Z 2021-05-18T08:41:48Z 1991 artículo http://purl.org/coar/resource_type/c_6501 Journal of Liquid Chromatography 14(13): 2457-2475 (1991) 0148-3919 http://hdl.handle.net/10261/241028 10.1080/01483919108049333 en https://doi.org/10.1080/01483919108049333 No none Taylor & Francis |
institution |
ICTAN ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-ictan-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del ICTAN España |
language |
English |
description |
The isocratic separation of 14 carotenoids, as well as retinol, retinyl acetate, retinyl palmitate, α-tocopherol and tocopherol acetate, is accomplished in 12 minutes, using a Spheri-5-ODS column and acetonitrile:dichloromethane:methanol (70:20:10) as mobile phase, with two-channel, programmable multiwavelength detection. The carotenoids separated are as follows: lutein/zeaxanthin, canthaxanthin, β-apo-8′ carotenal, β-cryptoxanthin, echinenone, lycopene, γ-carotene, α-carotene, β-carotene, 9-cis-β-carotene, 15-cis-/13-cis-β-carotene, phytoene and phytofluene. The separation of lutein and zeaxanthin is obtained simply by changing the mobile phase to acetonitrile:methanol (85:15). |
format |
artículo |
author |
Granado Lorencio, Fernando Olmedilla Alonso, Begoña Blanco, Inmaculada Rojas-Hidalgo, E. |
spellingShingle |
Granado Lorencio, Fernando Olmedilla Alonso, Begoña Blanco, Inmaculada Rojas-Hidalgo, E. An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene |
author_facet |
Granado Lorencio, Fernando Olmedilla Alonso, Begoña Blanco, Inmaculada Rojas-Hidalgo, E. |
author_sort |
Granado Lorencio, Fernando |
title |
An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene |
title_short |
An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene |
title_full |
An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene |
title_fullStr |
An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene |
title_full_unstemmed |
An improved HPLC method for the separation of fourteen carotenoids, including 15-/13- and 9-CIS-β-carotene Isomers, phytoene and phytofluene |
title_sort |
improved hplc method for the separation of fourteen carotenoids, including 15-/13- and 9-cis-β-carotene isomers, phytoene and phytofluene |
publisher |
Taylor & Francis |
publishDate |
1991 |
url |
http://hdl.handle.net/10261/241028 |
work_keys_str_mv |
AT granadolorenciofernando animprovedhplcmethodfortheseparationoffourteencarotenoidsincluding1513and9cisbcaroteneisomersphytoeneandphytofluene AT olmedillaalonsobegona animprovedhplcmethodfortheseparationoffourteencarotenoidsincluding1513and9cisbcaroteneisomersphytoeneandphytofluene AT blancoinmaculada animprovedhplcmethodfortheseparationoffourteencarotenoidsincluding1513and9cisbcaroteneisomersphytoeneandphytofluene AT rojashidalgoe animprovedhplcmethodfortheseparationoffourteencarotenoidsincluding1513and9cisbcaroteneisomersphytoeneandphytofluene AT granadolorenciofernando improvedhplcmethodfortheseparationoffourteencarotenoidsincluding1513and9cisbcaroteneisomersphytoeneandphytofluene AT olmedillaalonsobegona improvedhplcmethodfortheseparationoffourteencarotenoidsincluding1513and9cisbcaroteneisomersphytoeneandphytofluene AT blancoinmaculada improvedhplcmethodfortheseparationoffourteencarotenoidsincluding1513and9cisbcaroteneisomersphytoeneandphytofluene AT rojashidalgoe improvedhplcmethodfortheseparationoffourteencarotenoidsincluding1513and9cisbcaroteneisomersphytoeneandphytofluene |
_version_ |
1777670690566045696 |