Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype

Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype

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This article belongs to the Special Issue Novel Omics Studies on Anisakid Nematodes.

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Main Authors: Arcos, Susana C., Robertson, Lee, Ciordia, Sergio, Sánchez Alonso, Isabel, Careche, Mercedes, Carballeda-Sangiao, Noelia, González Muñoz, Miguel, Navas, Alfonso
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Subjects:Anisakis simplex s.s, Biomarkers, A. pegreffii, Proteins, Quantitative proteomics, iTRAQ,
Online Access:http://hdl.handle.net/10261/238078
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100003329
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spelling dig-ictan-es-10261-2380782021-12-27T15:55:28Z Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype Arcos, Susana C. Robertson, Lee Ciordia, Sergio Sánchez Alonso, Isabel Careche, Mercedes Carballeda-Sangiao, Noelia González Muñoz, Miguel Navas, Alfonso Ministerio de Economía y Competitividad (España) Instituto de Salud Carlos III Anisakis simplex s.s Biomarkers A. pegreffii Proteins Quantitative proteomics iTRAQ This article belongs to the Special Issue Novel Omics Studies on Anisakid Nematodes. The total proteomes of Anisakis simplex s.s., A. pegreffii and their hybrid genotype have been compared by quantitative proteomics (iTRAQ approach), which considers the level of expressed proteins. Comparison was made by means of two independent experiments considering four biological replicates of A. simplex and two each for A. pegreffii and hybrid between both species. A total of 1811 and 1976 proteins have been respectively identified in the experiments using public databases. One hundred ninety-six proteins were found significantly differentially expressed, and their relationships with the nematodes’ biological replicates were estimated by a multidimensional statistical approach. Results of pairwise Log2 ratio comparisons among them were statistically treated and supported in order to convert them into discrete character states. Principal component analysis (PCA) confirms the validity of the method. This comparison selected thirty seven proteins as discriminant taxonomic biomarkers among A. simplex, A. pegreffii and their hybrid genotype; 19 of these biomarkers, encoded by ten loci, are specific allergens of Anisakis (Ani s7, Ani s8, Ani s12, and Ani s14) and other (Ancylostoma secreted) is a common nematodes venom allergen. The rest of the markers comprise four unknown or non-characterized proteins; five different proteins (leucine) related to innate immunity, four proteolytic proteins (metalloendopeptidases), a lipase, a mitochondrial translocase protein, a neurotransmitter, a thyroxine transporter, and a structural collagen protein. The proposed methodology (proteomics and statistical) solidly characterize a set of proteins that are susceptible to take advantage of the new targeted proteomics. This work was partially supported by Spanish National Project AGL2015-68248-C2-1-R and the National Natural Science Museum. The proteomic analysis was performed in the Proteomics Facility of the Spanish National Center for Biotechnology (CNB-CSIC) that belongs to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019. Peer reviewed 2021-04-14T11:55:03Z 2021-04-14T11:55:03Z 2020 artículo http://purl.org/coar/resource_type/c_6501 Genes 11(8): 913 (2020) http://hdl.handle.net/10261/238078 10.3390/genes11080913 2073-4425 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100003329 32785065 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-68248-C2-1-R Publisher's version https://doi.org/10.3390/genes11080913 Sí open Multidisciplinary Digital Publishing Institute
institution ICTAN ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ictan-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICTAN España
language English
topic Anisakis simplex s.s
Biomarkers
A. pegreffii
Proteins
Quantitative proteomics
iTRAQ
Anisakis simplex s.s
Biomarkers
A. pegreffii
Proteins
Quantitative proteomics
iTRAQ
spellingShingle Anisakis simplex s.s
Biomarkers
A. pegreffii
Proteins
Quantitative proteomics
iTRAQ
Anisakis simplex s.s
Biomarkers
A. pegreffii
Proteins
Quantitative proteomics
iTRAQ
Arcos, Susana C.
Robertson, Lee
Ciordia, Sergio
Sánchez Alonso, Isabel
Careche, Mercedes
Carballeda-Sangiao, Noelia
González Muñoz, Miguel
Navas, Alfonso
Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype
description This article belongs to the Special Issue Novel Omics Studies on Anisakid Nematodes.
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Arcos, Susana C.
Robertson, Lee
Ciordia, Sergio
Sánchez Alonso, Isabel
Careche, Mercedes
Carballeda-Sangiao, Noelia
González Muñoz, Miguel
Navas, Alfonso
format artículo
topic_facet Anisakis simplex s.s
Biomarkers
A. pegreffii
Proteins
Quantitative proteomics
iTRAQ
author Arcos, Susana C.
Robertson, Lee
Ciordia, Sergio
Sánchez Alonso, Isabel
Careche, Mercedes
Carballeda-Sangiao, Noelia
González Muñoz, Miguel
Navas, Alfonso
author_sort Arcos, Susana C.
title Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype
title_short Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype
title_full Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype
title_fullStr Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype
title_full_unstemmed Quantitative proteomics comparison of total expressed proteomes of Anisakis simplex Sensu Stricto, A. pegreffii, and their hybrid genotype
title_sort quantitative proteomics comparison of total expressed proteomes of anisakis simplex sensu stricto, a. pegreffii, and their hybrid genotype
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://hdl.handle.net/10261/238078
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100003329
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