Immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis
In plant and animal nematode parasites, proteins derived from esophageal gland cells have been shown to be important in the host-nematodes relationship but little is known about the allergenic potential of these proteins in the genus Anisakis. Taking into account the increase of anisakiasis and allergies related to these nematodes, immunoreactive properties of gland cell proteins were investigated. Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining which revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp.-allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, with 82 spots revealed in 2D. The corresponding protein bands and spots were excised from the silver-stained gel and protein assignation was made by MALDI-TOF/TOF. A total of 13 (including proteoforms) were unambiguously identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin and one is an Anisakis simplex allergen.
Main Authors: | , , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Published: |
Multidisciplinary Digital Publishing Institute
2020-06-22
|
Subjects: | Anisakis simplex, Allergens, Esophageal glands, Proteomics, Excretion/secretion, |
Online Access: | http://hdl.handle.net/10261/215676 http://dx.doi.org/10.13039/501100003329 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-ictan-es-10261-215676 |
---|---|
record_format |
koha |
spelling |
dig-ictan-es-10261-2156762022-01-03T12:07:17Z Immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis Robertson, Lee Arcos, Susana C. Ciordia, Sergio Carballeda-Sangiao, Noelia Mena, M. Carmen Sánchez Alonso, Isabel González Muñoz, Miguel Careche, Mercedes Navas, Alfonso CSIC - Museo Nacional de Ciencias Naturales (MNCN) Ministerio de Economía y Competitividad (España) Anisakis simplex Allergens Esophageal glands Proteomics Excretion/secretion In plant and animal nematode parasites, proteins derived from esophageal gland cells have been shown to be important in the host-nematodes relationship but little is known about the allergenic potential of these proteins in the genus Anisakis. Taking into account the increase of anisakiasis and allergies related to these nematodes, immunoreactive properties of gland cell proteins were investigated. Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining which revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp.-allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, with 82 spots revealed in 2D. The corresponding protein bands and spots were excised from the silver-stained gel and protein assignation was made by MALDI-TOF/TOF. A total of 13 (including proteoforms) were unambiguously identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin and one is an Anisakis simplex allergen. This work was partially supported by Spanish National project AGL2015-68248-C2-1-R and National Natural Science Museum. The proteomic analysis was performed in the Proteomics Facility of The Spanish National Center for Biotechnology (CNB-CSIC) that belongs to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019. Peer reviewed 2020-06-30T16:37:12Z 2020-06-30T16:37:12Z 2020-06-22 2020-06-30T16:37:12Z artículo http://purl.org/coar/resource_type/c_6501 Genes 11(6): 683 (2020) http://hdl.handle.net/10261/215676 10.3390/genes11060683 2073-4425 http://dx.doi.org/10.13039/501100003329 32580523 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-68248-C2-1-R Publisher's version https://doi.org/10.3390/genes11060683 Sí open Multidisciplinary Digital Publishing Institute |
institution |
ICTAN ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-ictan-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del ICTAN España |
topic |
Anisakis simplex Allergens Esophageal glands Proteomics Excretion/secretion Anisakis simplex Allergens Esophageal glands Proteomics Excretion/secretion |
spellingShingle |
Anisakis simplex Allergens Esophageal glands Proteomics Excretion/secretion Anisakis simplex Allergens Esophageal glands Proteomics Excretion/secretion Robertson, Lee Arcos, Susana C. Ciordia, Sergio Carballeda-Sangiao, Noelia Mena, M. Carmen Sánchez Alonso, Isabel González Muñoz, Miguel Careche, Mercedes Navas, Alfonso Immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis |
description |
In plant and animal nematode parasites, proteins derived from esophageal gland cells have been shown to be important in the host-nematodes relationship but little is known about the allergenic potential of these proteins in the genus Anisakis. Taking into account the increase of anisakiasis and allergies related to these nematodes, immunoreactive properties of gland cell proteins were investigated. Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining which revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp.-allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, with 82 spots revealed in 2D. The corresponding protein bands and spots were excised from the silver-stained gel and protein assignation was made by MALDI-TOF/TOF. A total of 13 (including proteoforms) were unambiguously identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin and one is an Anisakis simplex allergen. |
author2 |
CSIC - Museo Nacional de Ciencias Naturales (MNCN) |
author_facet |
CSIC - Museo Nacional de Ciencias Naturales (MNCN) Robertson, Lee Arcos, Susana C. Ciordia, Sergio Carballeda-Sangiao, Noelia Mena, M. Carmen Sánchez Alonso, Isabel González Muñoz, Miguel Careche, Mercedes Navas, Alfonso |
format |
artículo |
topic_facet |
Anisakis simplex Allergens Esophageal glands Proteomics Excretion/secretion |
author |
Robertson, Lee Arcos, Susana C. Ciordia, Sergio Carballeda-Sangiao, Noelia Mena, M. Carmen Sánchez Alonso, Isabel González Muñoz, Miguel Careche, Mercedes Navas, Alfonso |
author_sort |
Robertson, Lee |
title |
Immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis |
title_short |
Immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis |
title_full |
Immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis |
title_fullStr |
Immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis |
title_full_unstemmed |
Immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis |
title_sort |
immunoreactive proteins in the esophageal gland cells of anisakis simplex sensu stricto detected by maldi-tof/tof analysis |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020-06-22 |
url |
http://hdl.handle.net/10261/215676 http://dx.doi.org/10.13039/501100003329 |
work_keys_str_mv |
AT robertsonlee immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis AT arcossusanac immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis AT ciordiasergio immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis AT carballedasangiaonoelia immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis AT menamcarmen immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis AT sanchezalonsoisabel immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis AT gonzalezmunozmiguel immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis AT carechemercedes immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis AT navasalfonso immunoreactiveproteinsintheesophagealglandcellsofanisakissimplexsensustrictodetectedbymalditoftofanalysis |
_version_ |
1777670659999006720 |