One-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents
Recent evidence has disclosed a connection between gut microbial glycosidase activity and adiposity in obese. Here, we measured microbial α-glucosidase and β-galactosidase activities and sorted fluorescently labeled β-galactosidase containing (βGAL) microorganisms in faecal samples of eight lean and thirteen obese adolescents that followed a controlled calorie restriction program during one year. β-galactosidase is a highly distributed functional trait, mainly expressed by members of Blautia, Bacteroides, Alcaligenes, Acinetobacter and Propionibacterium. Only long-term calorie restriction induced clear changes in the microbiota of obese adolescents. Long-term calorie restriction induced significant shifts in total and βGAL gut microbiota, reducing the Firmicutes:Bacteroidetes ratio and enhancing the growth of beneficial microorganisms such as Bacteroides, Roseburia, Faecalibacterium and Clostridium XIVa. Moreover, the structure and composition of βGAL community in obese after long-term calorie restriction was highly similar to that of lean adolescents. However, despite this high compositional similarity, microbial metabolic performance was different, split in two metabolic states at a body mass index value of 25. Our study shows that calorie restriction is a strong environmental force reshaping gut microbiota though its metabolic performance is linked to host's adiposity, suggesting that functional redundancy and metabolic plasticity are fundamental properties of gut microbial ecosystem.
Main Authors: | , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Published: |
Society for Applied Microbiology
2017
|
Online Access: | http://hdl.handle.net/10261/171448 http://dx.doi.org/10.13039/501100006318 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100010198 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-ictan-es-10261-171448 |
---|---|
record_format |
koha |
spelling |
dig-ictan-es-10261-1714482020-12-10T15:47:47Z One-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents Ruiz, Alicia Cerdó, Tomás Jáuregui, Ruy Pieper, Dietmar H. Marcos, Ascensión Clemente, Alfonso García, Federico Margolles Barros, Abelardo Ferrer, Manuel Campoy, Cristina Suárez, Antonio Ministerio de Economía, Industria y Competitividad (España) Ministerio de Economía y Competitividad (España) Universidad Carlos III de Madrid Instituto de Salud Carlos III Recent evidence has disclosed a connection between gut microbial glycosidase activity and adiposity in obese. Here, we measured microbial α-glucosidase and β-galactosidase activities and sorted fluorescently labeled β-galactosidase containing (βGAL) microorganisms in faecal samples of eight lean and thirteen obese adolescents that followed a controlled calorie restriction program during one year. β-galactosidase is a highly distributed functional trait, mainly expressed by members of Blautia, Bacteroides, Alcaligenes, Acinetobacter and Propionibacterium. Only long-term calorie restriction induced clear changes in the microbiota of obese adolescents. Long-term calorie restriction induced significant shifts in total and βGAL gut microbiota, reducing the Firmicutes:Bacteroidetes ratio and enhancing the growth of beneficial microorganisms such as Bacteroides, Roseburia, Faecalibacterium and Clostridium XIVa. Moreover, the structure and composition of βGAL community in obese after long-term calorie restriction was highly similar to that of lean adolescents. However, despite this high compositional similarity, microbial metabolic performance was different, split in two metabolic states at a body mass index value of 25. Our study shows that calorie restriction is a strong environmental force reshaping gut microbiota though its metabolic performance is linked to host's adiposity, suggesting that functional redundancy and metabolic plasticity are fundamental properties of gut microbial ecosystem. This work was funded by Carlos III Institute of Health, (Grants PI 051579, PI021513) and the Economy, Industry and Competitiveness Ministry (Grant AGL-11697/ALI). AR and TC received PhD FPU and FPI fellowships, respectively, from the Spanish Ministery of Economy and Competitivity. TC participated in the PhD Program in Biomedicine of the University. Peer Reviewed 2018-10-24T07:18:08Z 2018-10-24T07:18:08Z 2017 2018-10-24T07:18:08Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1111/1462-2920.13713 e-issn: 1462-2920 issn: 1462-2912 Environmental Microbiology 19(4): 1536-1551 (2017) http://hdl.handle.net/10261/171448 10.1111/1462-2920.13713 http://dx.doi.org/10.13039/501100006318 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100010198 28251782 Sí none Society for Applied Microbiology John Wiley & Sons |
institution |
ICTAN ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-ictan-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del ICTAN España |
description |
Recent evidence has disclosed a connection between gut microbial glycosidase activity and adiposity in obese. Here, we measured microbial α-glucosidase and β-galactosidase activities and sorted fluorescently labeled β-galactosidase containing (βGAL) microorganisms in faecal samples of eight lean and thirteen obese adolescents that followed a controlled calorie restriction program during one year. β-galactosidase is a highly distributed functional trait, mainly expressed by members of Blautia, Bacteroides, Alcaligenes, Acinetobacter and Propionibacterium. Only long-term calorie restriction induced clear changes in the microbiota of obese adolescents. Long-term calorie restriction induced significant shifts in total and βGAL gut microbiota, reducing the Firmicutes:Bacteroidetes ratio and enhancing the growth of beneficial microorganisms such as Bacteroides, Roseburia, Faecalibacterium and Clostridium XIVa. Moreover, the structure and composition of βGAL community in obese after long-term calorie restriction was highly similar to that of lean adolescents. However, despite this high compositional similarity, microbial metabolic performance was different, split in two metabolic states at a body mass index value of 25. Our study shows that calorie restriction is a strong environmental force reshaping gut microbiota though its metabolic performance is linked to host's adiposity, suggesting that functional redundancy and metabolic plasticity are fundamental properties of gut microbial ecosystem. |
author2 |
Ministerio de Economía, Industria y Competitividad (España) |
author_facet |
Ministerio de Economía, Industria y Competitividad (España) Ruiz, Alicia Cerdó, Tomás Jáuregui, Ruy Pieper, Dietmar H. Marcos, Ascensión Clemente, Alfonso García, Federico Margolles Barros, Abelardo Ferrer, Manuel Campoy, Cristina Suárez, Antonio |
format |
artículo |
author |
Ruiz, Alicia Cerdó, Tomás Jáuregui, Ruy Pieper, Dietmar H. Marcos, Ascensión Clemente, Alfonso García, Federico Margolles Barros, Abelardo Ferrer, Manuel Campoy, Cristina Suárez, Antonio |
spellingShingle |
Ruiz, Alicia Cerdó, Tomás Jáuregui, Ruy Pieper, Dietmar H. Marcos, Ascensión Clemente, Alfonso García, Federico Margolles Barros, Abelardo Ferrer, Manuel Campoy, Cristina Suárez, Antonio One-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents |
author_sort |
Ruiz, Alicia |
title |
One-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents |
title_short |
One-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents |
title_full |
One-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents |
title_fullStr |
One-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents |
title_full_unstemmed |
One-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents |
title_sort |
one-year calorie restriction impacts gut microbial composition but not its metabolic performance in obese adolescents |
publisher |
Society for Applied Microbiology |
publishDate |
2017 |
url |
http://hdl.handle.net/10261/171448 http://dx.doi.org/10.13039/501100006318 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100010198 |
work_keys_str_mv |
AT ruizalicia oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT cerdotomas oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT jaureguiruy oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT pieperdietmarh oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT marcosascension oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT clementealfonso oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT garciafederico oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT margollesbarrosabelardo oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT ferrermanuel oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT campoycristina oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents AT suarezantonio oneyearcalorierestrictionimpactsgutmicrobialcompositionbutnotitsmetabolicperformanceinobeseadolescents |
_version_ |
1777670579074105344 |