Determination of soluble dietary fibre content of Okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (AOAC and HPLC-ELSD)
Okara is a promising by-product from soybean and a rich source of dietary fibre, chiefly insoluble (IDF). To increase its solubility and functionality, a treatment with high hydrostatic pressure (HHP) assisted by food grade enzymes -Ultraflo L or Viscozyme L- has been performed. To monitor the effectiveness, an analysis of dietary fibre, mainly the soluble fraction (SDF), was accomplished by the AOAC enzymatic–gravimetric method with dialysis followed by both, spectrophotometric methods and High Performance Liquid Chromatography with Evaporative Light Scattering Detector (HPLC-ELSD) analysis of the soluble fraction. A significant increase in SDF (≈1.5-times) to the expense of a decrease in IDF was shown and chromatograms revealed two peaks of 95 and 22 kDa. Thus, treated Okara possessed a more balanced and convenient ratio of soluble to insoluble dietary fibre, which could have health benefits as prebiotic. Inbuilt interferences of the official AOAC’s method for dietary fibre were confirmed and the direct HPLC-ELSD approach was about twice more sensitive than spectrophotometric methods. Consequently, the direct HPLC-ELSD analysis of the supernatant is proposed as a cheaper, faster and reliable method. Combined HHP-treatments plus specific enzymes represent a promising alternative for the valorisation and preservation of agrofood by-products.
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Springer Nature
2017
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Subjects: | Ultraflo® L, Agrofood by-product, HHP, Okara, Viscozyme® L, SDF analysis, |
Online Access: | http://hdl.handle.net/10261/171231 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003086 |
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dig-ictan-es-10261-1712312021-12-27T16:29:33Z Determination of soluble dietary fibre content of Okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (AOAC and HPLC-ELSD) Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar Ministerio de Economía y Competitividad (España) Eusko Jaurlaritza Ultraflo® L Agrofood by-product HHP Okara Viscozyme® L SDF analysis Okara is a promising by-product from soybean and a rich source of dietary fibre, chiefly insoluble (IDF). To increase its solubility and functionality, a treatment with high hydrostatic pressure (HHP) assisted by food grade enzymes -Ultraflo L or Viscozyme L- has been performed. To monitor the effectiveness, an analysis of dietary fibre, mainly the soluble fraction (SDF), was accomplished by the AOAC enzymatic–gravimetric method with dialysis followed by both, spectrophotometric methods and High Performance Liquid Chromatography with Evaporative Light Scattering Detector (HPLC-ELSD) analysis of the soluble fraction. A significant increase in SDF (≈1.5-times) to the expense of a decrease in IDF was shown and chromatograms revealed two peaks of 95 and 22 kDa. Thus, treated Okara possessed a more balanced and convenient ratio of soluble to insoluble dietary fibre, which could have health benefits as prebiotic. Inbuilt interferences of the official AOAC’s method for dietary fibre were confirmed and the direct HPLC-ELSD approach was about twice more sensitive than spectrophotometric methods. Consequently, the direct HPLC-ELSD analysis of the supernatant is proposed as a cheaper, faster and reliable method. Combined HHP-treatments plus specific enzymes represent a promising alternative for the valorisation and preservation of agrofood by-products. Project AGL2016-77056-R from the Spanish MINECO supported this research. Elena Pérez-López acknowledges the predoctoral training programme of the Education, Language Policy and Culture of the Basque Government (Spain) (Grant No. PRE_2013_1_682) for her work experience contract at the Department of Metabolism and Nutrition of ICTAN-CSIC in Madrid. Peer Reviewed 2018-10-18T11:39:20Z 2018-10-18T11:39:20Z 2017 2018-10-18T11:39:20Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1007/s13197-017-2494-y e-issn: 0975-8402 issn: 0022-1155 Journal of Food Science and Technology 54(5): 1333-1339 (2017) http://hdl.handle.net/10261/171231 10.1007/s13197-017-2494-y http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003086 28416884 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-77056-R Sí none Springer Nature |
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Ultraflo® L Agrofood by-product HHP Okara Viscozyme® L SDF analysis Ultraflo® L Agrofood by-product HHP Okara Viscozyme® L SDF analysis |
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Ultraflo® L Agrofood by-product HHP Okara Viscozyme® L SDF analysis Ultraflo® L Agrofood by-product HHP Okara Viscozyme® L SDF analysis Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar Determination of soluble dietary fibre content of Okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (AOAC and HPLC-ELSD) |
description |
Okara is a promising by-product from soybean and a rich source of dietary fibre, chiefly insoluble (IDF). To increase its solubility and functionality, a treatment with high hydrostatic pressure (HHP) assisted by food grade enzymes -Ultraflo L or Viscozyme L- has been performed. To monitor the effectiveness, an analysis of dietary fibre, mainly the soluble fraction (SDF), was accomplished by the AOAC enzymatic–gravimetric method with dialysis followed by both, spectrophotometric methods and High Performance Liquid Chromatography with Evaporative Light Scattering Detector (HPLC-ELSD) analysis of the soluble fraction. A significant increase in SDF (≈1.5-times) to the expense of a decrease in IDF was shown and chromatograms revealed two peaks of 95 and 22 kDa. Thus, treated Okara possessed a more balanced and convenient ratio of soluble to insoluble dietary fibre, which could have health benefits as prebiotic. Inbuilt interferences of the official AOAC’s method for dietary fibre were confirmed and the direct HPLC-ELSD approach was about twice more sensitive than spectrophotometric methods. Consequently, the direct HPLC-ELSD analysis of the supernatant is proposed as a cheaper, faster and reliable method. Combined HHP-treatments plus specific enzymes represent a promising alternative for the valorisation and preservation of agrofood by-products. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar |
format |
artículo |
topic_facet |
Ultraflo® L Agrofood by-product HHP Okara Viscozyme® L SDF analysis |
author |
Pérez-López, Elena Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar |
author_sort |
Pérez-López, Elena |
title |
Determination of soluble dietary fibre content of Okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (AOAC and HPLC-ELSD) |
title_short |
Determination of soluble dietary fibre content of Okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (AOAC and HPLC-ELSD) |
title_full |
Determination of soluble dietary fibre content of Okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (AOAC and HPLC-ELSD) |
title_fullStr |
Determination of soluble dietary fibre content of Okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (AOAC and HPLC-ELSD) |
title_full_unstemmed |
Determination of soluble dietary fibre content of Okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (AOAC and HPLC-ELSD) |
title_sort |
determination of soluble dietary fibre content of okara treated with high hydrostatic pressure and enzymes: a comparative evaluation of two methods (aoac and hplc-elsd) |
publisher |
Springer Nature |
publishDate |
2017 |
url |
http://hdl.handle.net/10261/171231 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003086 |
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