In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota
At present, there is a huge interest in finding new prebiotics from agrofood industrial waste, such as the soyabean by-product Okara, rich in insoluble dietary fibre. A previous treatment of Okara with high hydrostatic pressure assisted by the food-grade enzyme Ultraflo L achieved a 58·2 % increment in its soluble dietary fibre (SDF) contents. Therefore, potential prebiotic effect of both treated and native Okara was assayed using 48 h, pH-controlled, anaerobic batch cultures inoculated with human faecal slurries, which simulate the human gut. Changes in faecal microbiota were evaluated using 16S rRNA-based fluorescence in situ hybridisation, whereas release of SCFA and lactic acid was assessed by HPLC. Both Okara samples exhibited potential prebiotic effects but Okara treated to maximise its SDF content showed higher SCFA plus lactic acid, better growth promotion of beneficial bacteria, including bifidobacteria after 4 and 48 h and lactobacilli after 4 h of fermentation, and a greater inhibition of potentially harmful bacterial groups such as clostridia and Bacteroides. Differences found between fructo-oligosaccharides and Okara substrates could be attributed to the great complexity of Okara's cell wall, which would need longer times to be fermented than other easily digested molecules, thus allowing an extended potential prebiotic effect. These results support an in vitro potential prebiotic effect of Okara.
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Format: | artículo biblioteca |
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Cambridge University Press
2016
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Subjects: | Okara, Hydrostatic pressure, Ultraflo® L enzyme, Fermentation, Microbiota, Prebiotics, Food by-products, |
Online Access: | http://hdl.handle.net/10261/140650 |
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dig-ictan-es-10261-1406502018-12-03T12:42:06Z In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota Pérez-López, Elena Cela, D. Costabile, Adele Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar Okara Hydrostatic pressure Ultraflo® L enzyme Fermentation Microbiota Prebiotics Food by-products At present, there is a huge interest in finding new prebiotics from agrofood industrial waste, such as the soyabean by-product Okara, rich in insoluble dietary fibre. A previous treatment of Okara with high hydrostatic pressure assisted by the food-grade enzyme Ultraflo L achieved a 58·2 % increment in its soluble dietary fibre (SDF) contents. Therefore, potential prebiotic effect of both treated and native Okara was assayed using 48 h, pH-controlled, anaerobic batch cultures inoculated with human faecal slurries, which simulate the human gut. Changes in faecal microbiota were evaluated using 16S rRNA-based fluorescence in situ hybridisation, whereas release of SCFA and lactic acid was assessed by HPLC. Both Okara samples exhibited potential prebiotic effects but Okara treated to maximise its SDF content showed higher SCFA plus lactic acid, better growth promotion of beneficial bacteria, including bifidobacteria after 4 and 48 h and lactobacilli after 4 h of fermentation, and a greater inhibition of potentially harmful bacterial groups such as clostridia and Bacteroides. Differences found between fructo-oligosaccharides and Okara substrates could be attributed to the great complexity of Okara's cell wall, which would need longer times to be fermented than other easily digested molecules, thus allowing an extended potential prebiotic effect. These results support an in vitro potential prebiotic effect of Okara. Peer Reviewed 2016-11-23T12:16:54Z 2016-11-23T12:16:54Z 2016 2016-11-23T12:16:54Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1017/S0007114516002816 issn: 1475-2662 British Journal of Nutrition 116: 1116- 1124 (2016) http://hdl.handle.net/10261/140650 10.1017/S0007114516002816 open Cambridge University Press |
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Okara Hydrostatic pressure Ultraflo® L enzyme Fermentation Microbiota Prebiotics Food by-products Okara Hydrostatic pressure Ultraflo® L enzyme Fermentation Microbiota Prebiotics Food by-products |
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Okara Hydrostatic pressure Ultraflo® L enzyme Fermentation Microbiota Prebiotics Food by-products Okara Hydrostatic pressure Ultraflo® L enzyme Fermentation Microbiota Prebiotics Food by-products Pérez-López, Elena Cela, D. Costabile, Adele Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota |
description |
At present, there is a huge interest in finding new prebiotics from agrofood industrial waste, such as the soyabean by-product Okara, rich in insoluble dietary fibre. A previous treatment of Okara with high hydrostatic pressure assisted by the food-grade enzyme Ultraflo L achieved a 58·2 % increment in its soluble dietary fibre (SDF) contents. Therefore, potential prebiotic effect of both treated and native Okara was assayed using 48 h, pH-controlled, anaerobic batch cultures inoculated with human faecal slurries, which simulate the human gut. Changes in faecal microbiota were evaluated using 16S rRNA-based fluorescence in situ hybridisation, whereas release of SCFA and lactic acid was assessed by HPLC. Both Okara samples exhibited potential prebiotic effects but Okara treated to maximise its SDF content showed higher SCFA plus lactic acid, better growth promotion of beneficial bacteria, including bifidobacteria after 4 and 48 h and lactobacilli after 4 h of fermentation, and a greater inhibition of potentially harmful bacterial groups such as clostridia and Bacteroides. Differences found between fructo-oligosaccharides and Okara substrates could be attributed to the great complexity of Okara's cell wall, which would need longer times to be fermented than other easily digested molecules, thus allowing an extended potential prebiotic effect. These results support an in vitro potential prebiotic effect of Okara. |
format |
artículo |
topic_facet |
Okara Hydrostatic pressure Ultraflo® L enzyme Fermentation Microbiota Prebiotics Food by-products |
author |
Pérez-López, Elena Cela, D. Costabile, Adele Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar |
author_facet |
Pérez-López, Elena Cela, D. Costabile, Adele Mateos-Aparicio, Inmaculada Rupérez Antón, Pilar |
author_sort |
Pérez-López, Elena |
title |
In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota |
title_short |
In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota |
title_full |
In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota |
title_fullStr |
In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota |
title_full_unstemmed |
In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota |
title_sort |
in vitro fermentability and prebiotic potential of soyabean okara by human faecal microbiota |
publisher |
Cambridge University Press |
publishDate |
2016 |
url |
http://hdl.handle.net/10261/140650 |
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