A new HPLC-PAD/HPLC-ESI-MS method for the analysis of phytoestrogens produced by bacterial metabolism
Phytoestrogens (isoflavones, ellagitannins, and lignans) are polyphenols with an estrogenic/antiestrogenic function present in fruits, vegetables, and foods derived from them. Some microorganisms are capable of transforming isoflavones, ellagitannins, and lignans into equol, urolithins, and enterolignans, respectively. These compounds are more bioavailable, they have more estrogenic/antiestrogenic and antioxidant activity than their precursors, and they are associated with beneficial effects for human health. Extracts of soy- bean and lignans and ellagic acid were incubated with feces, or bacteria isolated from feces, and later the phytoestrogens were extracted twice with diethyl ether and twice with ethyl acetate, and the solvents were evaporated at room temperature under a N2. Subsequently, phytoestrogens were detected by HPLC-PAD and chromatographic peaks were identified by HPLC-ESI/M, and they were confirmed by comparison of retention times and characteristics of UV spectra with those of standards. Analysis of phytoestrogens by means of HPLC- PAD allowed the simultaneous detection of isoflavones, ellagitannins, and lignans metabolized, or not, by intestinal microbiota with high sensitivity (LOD: equol 0.11 mg/L; urolithin A 0.17 mg/L, enterodiol 0.15 mg/L). Assays were linear up to at least 20 mg/L in all standards with a high correlation (equol >0.999; urolithin A >0.998, enterodiol >0.999). Therefore, the described method constitutes an affordable and easy alternative method for the detection of phytoestrogen metabolism. Moreover, this method could be applied to the detection of isoflavones, lignans, and ellagitannins in food.
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Springer
2016
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Subjects: | HPLC-PAD, Urolithins, Enterolignans, Equol, HPLC-ESI/MS, |
Online Access: | http://hdl.handle.net/10261/134957 http://dx.doi.org/10.13039/501100003329 |
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dig-ictan-es-10261-1349572020-12-10T11:41:04Z A new HPLC-PAD/HPLC-ESI-MS method for the analysis of phytoestrogens produced by bacterial metabolism Gaya, Pilar Arqués, Juan Luis Medina, Margarita Álvarez Acero, Inmaculada Landete, José María Ministerio de Economía y Competitividad (España) HPLC-PAD Urolithins Enterolignans Equol HPLC-ESI/MS Phytoestrogens (isoflavones, ellagitannins, and lignans) are polyphenols with an estrogenic/antiestrogenic function present in fruits, vegetables, and foods derived from them. Some microorganisms are capable of transforming isoflavones, ellagitannins, and lignans into equol, urolithins, and enterolignans, respectively. These compounds are more bioavailable, they have more estrogenic/antiestrogenic and antioxidant activity than their precursors, and they are associated with beneficial effects for human health. Extracts of soy- bean and lignans and ellagic acid were incubated with feces, or bacteria isolated from feces, and later the phytoestrogens were extracted twice with diethyl ether and twice with ethyl acetate, and the solvents were evaporated at room temperature under a N2. Subsequently, phytoestrogens were detected by HPLC-PAD and chromatographic peaks were identified by HPLC-ESI/M, and they were confirmed by comparison of retention times and characteristics of UV spectra with those of standards. Analysis of phytoestrogens by means of HPLC- PAD allowed the simultaneous detection of isoflavones, ellagitannins, and lignans metabolized, or not, by intestinal microbiota with high sensitivity (LOD: equol 0.11 mg/L; urolithin A 0.17 mg/L, enterodiol 0.15 mg/L). Assays were linear up to at least 20 mg/L in all standards with a high correlation (equol >0.999; urolithin A >0.998, enterodiol >0.999). Therefore, the described method constitutes an affordable and easy alternative method for the detection of phytoestrogen metabolism. Moreover, this method could be applied to the detection of isoflavones, lignans, and ellagitannins in food. This work was supported by RM2012-00004-00-00. J.M. Landete has a postdoctoral contract with the research program “Ramón y Cajal” (MINECO, Spain). Peer Reviewed 2016-07-21T09:55:47Z 2016-07-21T09:55:47Z 2016 2016-07-21T09:55:47Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1007/s12161-015-0226-3 issn: 1936-976X Food Analytical Methods 9(2): 537-547 (2016) http://hdl.handle.net/10261/134957 10.1007/s12161-015-0226-3 http://dx.doi.org/10.13039/501100003329 Sí none Springer |
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HPLC-PAD Urolithins Enterolignans Equol HPLC-ESI/MS HPLC-PAD Urolithins Enterolignans Equol HPLC-ESI/MS |
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HPLC-PAD Urolithins Enterolignans Equol HPLC-ESI/MS HPLC-PAD Urolithins Enterolignans Equol HPLC-ESI/MS Gaya, Pilar Arqués, Juan Luis Medina, Margarita Álvarez Acero, Inmaculada Landete, José María A new HPLC-PAD/HPLC-ESI-MS method for the analysis of phytoestrogens produced by bacterial metabolism |
description |
Phytoestrogens (isoflavones, ellagitannins, and lignans) are polyphenols with an estrogenic/antiestrogenic function present in fruits, vegetables, and foods derived from them. Some microorganisms are capable of transforming isoflavones, ellagitannins, and lignans into equol, urolithins, and enterolignans, respectively. These compounds are more bioavailable, they have more estrogenic/antiestrogenic and antioxidant activity than their precursors, and they are associated with beneficial effects for human health. Extracts of soy- bean and lignans and ellagic acid were incubated with feces, or bacteria isolated from feces, and later the phytoestrogens were extracted twice with diethyl ether and twice with ethyl acetate, and the solvents were evaporated at room temperature under a N2. Subsequently, phytoestrogens were detected by HPLC-PAD and chromatographic peaks were identified by HPLC-ESI/M, and they were confirmed by comparison of retention times and characteristics of UV spectra with those of standards. Analysis of phytoestrogens by means of HPLC- PAD allowed the simultaneous detection of isoflavones, ellagitannins, and lignans metabolized, or not, by intestinal microbiota with high sensitivity (LOD: equol 0.11 mg/L; urolithin A 0.17 mg/L, enterodiol 0.15 mg/L). Assays were linear up to at least 20 mg/L in all standards with a high correlation (equol >0.999; urolithin A >0.998, enterodiol >0.999). Therefore, the described method constitutes an affordable and easy alternative method for the detection of phytoestrogen metabolism. Moreover, this method could be applied to the detection of isoflavones, lignans, and ellagitannins in food. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Gaya, Pilar Arqués, Juan Luis Medina, Margarita Álvarez Acero, Inmaculada Landete, José María |
format |
artículo |
topic_facet |
HPLC-PAD Urolithins Enterolignans Equol HPLC-ESI/MS |
author |
Gaya, Pilar Arqués, Juan Luis Medina, Margarita Álvarez Acero, Inmaculada Landete, José María |
author_sort |
Gaya, Pilar |
title |
A new HPLC-PAD/HPLC-ESI-MS method for the analysis of phytoestrogens produced by bacterial metabolism |
title_short |
A new HPLC-PAD/HPLC-ESI-MS method for the analysis of phytoestrogens produced by bacterial metabolism |
title_full |
A new HPLC-PAD/HPLC-ESI-MS method for the analysis of phytoestrogens produced by bacterial metabolism |
title_fullStr |
A new HPLC-PAD/HPLC-ESI-MS method for the analysis of phytoestrogens produced by bacterial metabolism |
title_full_unstemmed |
A new HPLC-PAD/HPLC-ESI-MS method for the analysis of phytoestrogens produced by bacterial metabolism |
title_sort |
new hplc-pad/hplc-esi-ms method for the analysis of phytoestrogens produced by bacterial metabolism |
publisher |
Springer |
publishDate |
2016 |
url |
http://hdl.handle.net/10261/134957 http://dx.doi.org/10.13039/501100003329 |
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