Metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (Caco-2) and hepatic (HepG2) cells
The purpose of this work was to study the absorption and metabolism of sulforaphane (SFN) from broccoli sprouts, their major glucosinolate glucoraphanin and its hydrolysis product SFN. This was done by monitoring SFN¿s main metabolites, i.e. SFN, SFN-glutathione and SFN-cysteine, in two different cell models of absorption and metabolism (Caco-2 colorectal carcinoma cells and HepG2 hepatocellular carcinoma cells), during 3, 6, and 24 h of treatment, using a selective UHPLC-QqQ-MS/MS procedure. Concentrations ranging 0.5¿90 nmol/l were found within the cells and released in the medium, depending on the type of analyte under study. Cells were capable of conjugative metabolism, since the SFN mercapturic derivatives could be identified in the cell medium. The antiproliferative activity of broccoli sprouts, glucoraphanin and sulforaphane was compared in Caco-2 and HT-29 human colorectal carcinoma cells, and HepG2 hepatocellular carcinoma cells, establishing the minimal concentration of a given compound to achieve half inhibition of the maximal cell growth (IC50) for broccoli sprouts extract and sulforaphane. However, glucoraphanin did not show an antiproliferative effect in the cells under study.
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Springer
2015
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Subjects: | Metabolism, Cytotoxicity, UHPLC-QqQ-MS/MS I, Brassica oleraceae, |
Online Access: | http://hdl.handle.net/10261/133573 http://dx.doi.org/10.13039/501100003329 |
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dig-ictan-es-10261-1335732021-06-30T10:52:38Z Metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (Caco-2) and hepatic (HepG2) cells Baenas, Nieves Silván, José Manuel Medina, Sonia Pascual-Teresa, Sonia de García-Viguera, Cristina Moreno, Diego A. Ministerio de Economía y Competitividad (España) Metabolism Cytotoxicity UHPLC-QqQ-MS/MS I Brassica oleraceae The purpose of this work was to study the absorption and metabolism of sulforaphane (SFN) from broccoli sprouts, their major glucosinolate glucoraphanin and its hydrolysis product SFN. This was done by monitoring SFN¿s main metabolites, i.e. SFN, SFN-glutathione and SFN-cysteine, in two different cell models of absorption and metabolism (Caco-2 colorectal carcinoma cells and HepG2 hepatocellular carcinoma cells), during 3, 6, and 24 h of treatment, using a selective UHPLC-QqQ-MS/MS procedure. Concentrations ranging 0.5¿90 nmol/l were found within the cells and released in the medium, depending on the type of analyte under study. Cells were capable of conjugative metabolism, since the SFN mercapturic derivatives could be identified in the cell medium. The antiproliferative activity of broccoli sprouts, glucoraphanin and sulforaphane was compared in Caco-2 and HT-29 human colorectal carcinoma cells, and HepG2 hepatocellular carcinoma cells, establishing the minimal concentration of a given compound to achieve half inhibition of the maximal cell growth (IC50) for broccoli sprouts extract and sulforaphane. However, glucoraphanin did not show an antiproliferative effect in the cells under study. This work was supported by the Spanish Ministry of Economy and Competitiveness through projects:AGL2012-30803 and AGL2013-46247-P, and the CYTED Program (Ref. 112RT0460) CORNUCOPIA Peer Reviewed 2016-06-16T09:52:13Z 2016-06-16T09:52:13Z 2015 2016-06-16T09:52:14Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1007/s11101-015-9422-4 issn: 1568-7767 Phytochemistry Reviews 14: 1035- 1044 (2015) http://hdl.handle.net/10261/133573 10.1007/s11101-015-9422-4 http://dx.doi.org/10.13039/501100003329 Sí none Springer |
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Metabolism Cytotoxicity UHPLC-QqQ-MS/MS I Brassica oleraceae Metabolism Cytotoxicity UHPLC-QqQ-MS/MS I Brassica oleraceae |
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Metabolism Cytotoxicity UHPLC-QqQ-MS/MS I Brassica oleraceae Metabolism Cytotoxicity UHPLC-QqQ-MS/MS I Brassica oleraceae Baenas, Nieves Silván, José Manuel Medina, Sonia Pascual-Teresa, Sonia de García-Viguera, Cristina Moreno, Diego A. Metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (Caco-2) and hepatic (HepG2) cells |
description |
The purpose of this work was to study the absorption and metabolism of sulforaphane (SFN) from broccoli sprouts, their major glucosinolate glucoraphanin and its hydrolysis product SFN. This was done by monitoring SFN¿s main metabolites, i.e. SFN, SFN-glutathione and SFN-cysteine, in two different cell models of absorption and metabolism (Caco-2 colorectal carcinoma cells and HepG2 hepatocellular carcinoma cells), during 3, 6, and 24 h of treatment, using a selective UHPLC-QqQ-MS/MS procedure. Concentrations ranging 0.5¿90 nmol/l were found within the cells and released in the medium, depending on the type of analyte under study. Cells were capable of conjugative metabolism, since the SFN mercapturic derivatives could be identified in the cell medium. The antiproliferative activity of broccoli sprouts, glucoraphanin and sulforaphane was compared in Caco-2 and HT-29 human colorectal carcinoma cells, and HepG2 hepatocellular carcinoma cells, establishing the minimal concentration of a given compound to achieve half inhibition of the maximal cell growth (IC50) for broccoli sprouts extract and sulforaphane. However, glucoraphanin did not show an antiproliferative effect in the cells under study. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Baenas, Nieves Silván, José Manuel Medina, Sonia Pascual-Teresa, Sonia de García-Viguera, Cristina Moreno, Diego A. |
format |
artículo |
topic_facet |
Metabolism Cytotoxicity UHPLC-QqQ-MS/MS I Brassica oleraceae |
author |
Baenas, Nieves Silván, José Manuel Medina, Sonia Pascual-Teresa, Sonia de García-Viguera, Cristina Moreno, Diego A. |
author_sort |
Baenas, Nieves |
title |
Metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (Caco-2) and hepatic (HepG2) cells |
title_short |
Metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (Caco-2) and hepatic (HepG2) cells |
title_full |
Metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (Caco-2) and hepatic (HepG2) cells |
title_fullStr |
Metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (Caco-2) and hepatic (HepG2) cells |
title_full_unstemmed |
Metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (Caco-2) and hepatic (HepG2) cells |
title_sort |
metabolism and antiproliferative effects of sulforaphane and broccoli sprouts in human intestinal (caco-2) and hepatic (hepg2) cells |
publisher |
Springer |
publishDate |
2015 |
url |
http://hdl.handle.net/10261/133573 http://dx.doi.org/10.13039/501100003329 |
work_keys_str_mv |
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