Anthocyanins do not influence long-chain n-3 fatty acid status: Studies in cells, rodents and humans
© 2015 The Authors. Increased tissue status of the long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) is associated with cardiovascular and cognitive benefits. Limited epidemiological and animal data suggest that flavonoids, and specifically anthocyanins, may increase EPA and DHA levels, potentially by increasing their synthesis from the shorter-chain n-3 PUFA, α-linolenic acid. Using complimentary cell, rodent and human studies we investigated the impact of anthocyanins and anthocyanin-rich foods/extracts on plasma and tissue EPA and DHA levels and on the expression of fatty acid desaturase 2 (FADS2), which represents the rate limiting enzymes in EPA and DHA synthesis. In experiment 1, rats were fed a standard diet containing either palm oil or rapeseed oil supplemented with pure anthocyanins for 8 weeks. Retrospective fatty acid analysis was conducted on plasma samples collected from a human randomized controlled trial where participants consumed an elderberry extract for 12 weeks (experiment 2). HepG2 cells were cultured with α-linolenic acid with or without select anthocyanins and their in vivo metabolites for 24 h and 48 h (experiment 3). The fatty acid composition of the cell membranes, plasma and liver tissues were analyzed by gas chromatography. Anthocyanins and anthocyanin-rich food intake had no significant impact on EPA or DHA status or FADS2 gene expression in any model system. These data indicate little impact of dietary anthocyanins on n-3 PUFA distribution and suggest that the increasingly recognized benefits of anthocyanins are unlikely to be the result of a beneficial impact on tissue fatty acid status.
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Elsevier
2015
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Subjects: | Liver, Rat, Human, n-3 PUFA, Anthocyanins, |
Online Access: | http://hdl.handle.net/10261/114897 |
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dig-ictan-es-10261-1148972021-12-28T16:18:16Z Anthocyanins do not influence long-chain n-3 fatty acid status: Studies in cells, rodents and humans Vauzour, David Pascual-Teresa, Sonia de Minihane, Anne-Marie Liver Rat Human n-3 PUFA Anthocyanins © 2015 The Authors. Increased tissue status of the long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) is associated with cardiovascular and cognitive benefits. Limited epidemiological and animal data suggest that flavonoids, and specifically anthocyanins, may increase EPA and DHA levels, potentially by increasing their synthesis from the shorter-chain n-3 PUFA, α-linolenic acid. Using complimentary cell, rodent and human studies we investigated the impact of anthocyanins and anthocyanin-rich foods/extracts on plasma and tissue EPA and DHA levels and on the expression of fatty acid desaturase 2 (FADS2), which represents the rate limiting enzymes in EPA and DHA synthesis. In experiment 1, rats were fed a standard diet containing either palm oil or rapeseed oil supplemented with pure anthocyanins for 8 weeks. Retrospective fatty acid analysis was conducted on plasma samples collected from a human randomized controlled trial where participants consumed an elderberry extract for 12 weeks (experiment 2). HepG2 cells were cultured with α-linolenic acid with or without select anthocyanins and their in vivo metabolites for 24 h and 48 h (experiment 3). The fatty acid composition of the cell membranes, plasma and liver tissues were analyzed by gas chromatography. Anthocyanins and anthocyanin-rich food intake had no significant impact on EPA or DHA status or FADS2 gene expression in any model system. These data indicate little impact of dietary anthocyanins on n-3 PUFA distribution and suggest that the increasingly recognized benefits of anthocyanins are unlikely to be the result of a beneficial impact on tissue fatty acid status. Peer Reviewed 2015-05-08T08:19:15Z 2015-05-08T08:19:15Z 2015 2015-05-08T08:19:15Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.jnutbio.2014.09.005 issn: 1873-4847 Journal of Nutritional Biochemistry 26: 211- 218 (2015) http://hdl.handle.net/10261/114897 10.1016/j.jnutbio.2014.09.005 25573539 open Elsevier |
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Liver Rat Human n-3 PUFA Anthocyanins Liver Rat Human n-3 PUFA Anthocyanins Vauzour, David Pascual-Teresa, Sonia de Minihane, Anne-Marie Anthocyanins do not influence long-chain n-3 fatty acid status: Studies in cells, rodents and humans |
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© 2015 The Authors. Increased tissue status of the long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA), eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) is associated with cardiovascular and cognitive benefits. Limited epidemiological and animal data suggest that flavonoids, and specifically anthocyanins, may increase EPA and DHA levels, potentially by increasing their synthesis from the shorter-chain n-3 PUFA, α-linolenic acid. Using complimentary cell, rodent and human studies we investigated the impact of anthocyanins and anthocyanin-rich foods/extracts on plasma and tissue EPA and DHA levels and on the expression of fatty acid desaturase 2 (FADS2), which represents the rate limiting enzymes in EPA and DHA synthesis. In experiment 1, rats were fed a standard diet containing either palm oil or rapeseed oil supplemented with pure anthocyanins for 8 weeks. Retrospective fatty acid analysis was conducted on plasma samples collected from a human randomized controlled trial where participants consumed an elderberry extract for 12 weeks (experiment 2). HepG2 cells were cultured with α-linolenic acid with or without select anthocyanins and their in vivo metabolites for 24 h and 48 h (experiment 3). The fatty acid composition of the cell membranes, plasma and liver tissues were analyzed by gas chromatography. Anthocyanins and anthocyanin-rich food intake had no significant impact on EPA or DHA status or FADS2 gene expression in any model system. These data indicate little impact of dietary anthocyanins on n-3 PUFA distribution and suggest that the increasingly recognized benefits of anthocyanins are unlikely to be the result of a beneficial impact on tissue fatty acid status. |
format |
artículo |
topic_facet |
Liver Rat Human n-3 PUFA Anthocyanins |
author |
Vauzour, David Pascual-Teresa, Sonia de Minihane, Anne-Marie |
author_facet |
Vauzour, David Pascual-Teresa, Sonia de Minihane, Anne-Marie |
author_sort |
Vauzour, David |
title |
Anthocyanins do not influence long-chain n-3 fatty acid status: Studies in cells, rodents and humans |
title_short |
Anthocyanins do not influence long-chain n-3 fatty acid status: Studies in cells, rodents and humans |
title_full |
Anthocyanins do not influence long-chain n-3 fatty acid status: Studies in cells, rodents and humans |
title_fullStr |
Anthocyanins do not influence long-chain n-3 fatty acid status: Studies in cells, rodents and humans |
title_full_unstemmed |
Anthocyanins do not influence long-chain n-3 fatty acid status: Studies in cells, rodents and humans |
title_sort |
anthocyanins do not influence long-chain n-3 fatty acid status: studies in cells, rodents and humans |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/10261/114897 |
work_keys_str_mv |
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