Characterisation of a cold-active and salt-tolerant esterase from Lactobacillus plantarum with potential application during cheese ripening
© 2014 Elsevier Ltd. During cheese ripening, the metabolic activity of microorganisms influences cheese flavour. Lactobacillus plantarum is a non-starter lactic acid bacterium that can be found during cheese ripening. An esterase-encoding gene from L. plantarum, lp_3505, was cloned and expressed in Escherichia coli BL21 (DE3). The biotechnologically produced Lp_3505 protein was purified as an active soluble form using His-tag affinity chromatography. The enzyme has an optimal pH and temperature of 6 and 5 °C, respectively. The enzyme showed remarkable stability at 20 °C, retaining more than 60% of its maximal activity after 20 h incubation at this temperature. In addition, NaCl concentrations lower than 20% increased esterase activity. The cold-activity and salt-tolerance exhibited by Lp_3505 indicated that this esterase could be a useful exogenous enzyme to be added for cheese ripening.
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2014-12
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Online Access: | http://hdl.handle.net/10261/103191 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 |
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dig-ictan-es-10261-1031912021-06-30T07:49:11Z Characterisation of a cold-active and salt-tolerant esterase from Lactobacillus plantarum with potential application during cheese ripening Esteban-Torres, María Santamaría, Laura Rivas, Blanca de las Muñoz, Rosario Ministerio de Economía y Competitividad (España) Comunidad de Madrid © 2014 Elsevier Ltd. During cheese ripening, the metabolic activity of microorganisms influences cheese flavour. Lactobacillus plantarum is a non-starter lactic acid bacterium that can be found during cheese ripening. An esterase-encoding gene from L. plantarum, lp_3505, was cloned and expressed in Escherichia coli BL21 (DE3). The biotechnologically produced Lp_3505 protein was purified as an active soluble form using His-tag affinity chromatography. The enzyme has an optimal pH and temperature of 6 and 5 °C, respectively. The enzyme showed remarkable stability at 20 °C, retaining more than 60% of its maximal activity after 20 h incubation at this temperature. In addition, NaCl concentrations lower than 20% increased esterase activity. The cold-activity and salt-tolerance exhibited by Lp_3505 indicated that this esterase could be a useful exogenous enzyme to be added for cheese ripening. This work was financially supported by grants AGL2011-22745 and BFU2010-17929 (MINECO), S2009/AGR-1469 (ALIBIRD) (Comunidad de Madrid), and RM2012-00004 (INIA) Peer Reviewed 2014-10-13T07:01:07Z 2014-10-13T07:01:07Z 2014-12 2014-10-13T07:01:08Z artículo http://purl.org/coar/resource_type/c_6501 issn: 0958-6946 International Dairy Journal 39(2): 312-315 (2014) http://hdl.handle.net/10261/103191 10.1016/j.idairyj.2014.08.004 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 #PLACEHOLDER_PARENT_METADATA_VALUE# S2009/AGR-1469/ALIBIRD Postprint http://dx.doi.org/10.1016/j.idairyj.2014.08.004 Sí open Elsevier |
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© 2014 Elsevier Ltd. During cheese ripening, the metabolic activity of microorganisms influences cheese flavour. Lactobacillus plantarum is a non-starter lactic acid bacterium that can be found during cheese ripening. An esterase-encoding gene from L. plantarum, lp_3505, was cloned and expressed in Escherichia coli BL21 (DE3). The biotechnologically produced Lp_3505 protein was purified as an active soluble form using His-tag affinity chromatography. The enzyme has an optimal pH and temperature of 6 and 5 °C, respectively. The enzyme showed remarkable stability at 20 °C, retaining more than 60% of its maximal activity after 20 h incubation at this temperature. In addition, NaCl concentrations lower than 20% increased esterase activity. The cold-activity and salt-tolerance exhibited by Lp_3505 indicated that this esterase could be a useful exogenous enzyme to be added for cheese ripening. |
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Ministerio de Economía y Competitividad (España) |
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Ministerio de Economía y Competitividad (España) Esteban-Torres, María Santamaría, Laura Rivas, Blanca de las Muñoz, Rosario |
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Esteban-Torres, María Santamaría, Laura Rivas, Blanca de las Muñoz, Rosario |
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Esteban-Torres, María Santamaría, Laura Rivas, Blanca de las Muñoz, Rosario Characterisation of a cold-active and salt-tolerant esterase from Lactobacillus plantarum with potential application during cheese ripening |
author_sort |
Esteban-Torres, María |
title |
Characterisation of a cold-active and salt-tolerant esterase from Lactobacillus plantarum with potential application during cheese ripening |
title_short |
Characterisation of a cold-active and salt-tolerant esterase from Lactobacillus plantarum with potential application during cheese ripening |
title_full |
Characterisation of a cold-active and salt-tolerant esterase from Lactobacillus plantarum with potential application during cheese ripening |
title_fullStr |
Characterisation of a cold-active and salt-tolerant esterase from Lactobacillus plantarum with potential application during cheese ripening |
title_full_unstemmed |
Characterisation of a cold-active and salt-tolerant esterase from Lactobacillus plantarum with potential application during cheese ripening |
title_sort |
characterisation of a cold-active and salt-tolerant esterase from lactobacillus plantarum with potential application during cheese ripening |
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Elsevier |
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2014-12 |
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http://hdl.handle.net/10261/103191 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 |
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