New Insights into the Diversity of Marine Picoeukaryotes

New Insights into the Diversity of Marine Picoeukaryotes

7 pages, 1 figure, 1 table

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Main Authors: Not, Fabrice, Campo, Javier del, Balagué, Vanessa, Vargas, Colomban de, Massana, Ramon
Format: artículo biblioteca
Language:English
Published: Public Library of Science 2009-09
Online Access:http://hdl.handle.net/10261/84047
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id dig-icm-es-10261-84047
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spelling dig-icm-es-10261-840472021-12-28T16:05:29Z New Insights into the Diversity of Marine Picoeukaryotes Not, Fabrice Campo, Javier del Balagué, Vanessa Vargas, Colomban de Massana, Ramon 7 pages, 1 figure, 1 table Over the last decade, culture-independent surveys of marine picoeukaryotic diversity based on 18S ribosomal DNA clone libraries have unveiled numerous sequences of novel high-rank taxa. This newfound diversity has significantly altered our understanding of marine microbial food webs and the evolution of eukaryotes. However, the current picture of marine eukaryotic biodiversity may be significantly skewed by PCR amplification biases, occurrence of rDNA genes in multiple copies within a single cell, and the capacity of DNA to persist as extracellular material. In this study we performed an analysis of the metagenomic dataset from the Global Ocean Survey (GOS) expedition, seeking eukaryotic ribosomal signatures. This PCR-free approach revealed similar phylogenetic patterns to clone library surveys, suggesting that PCR steps do not impose major biases in the exploration of environmental DNA. The different cell size fractions within the GOS dataset, however, displayed a distinct picture. High protistan diversity in the <0.8 μm size fraction, in particular sequences from radiolarians and ciliates (and their absence in the 0.8-3 μm fraction), suggest that most of the DNA in this fraction comes from extracellular material from larger cells. In addition, we compared the phylogenetic patterns from rDNA and reverse transcribed rRNA 18S clone libraries from the same sample harvested in the Mediterranean Sea. The libraries revealed major differences, with taxa such as pelagophytes or picobiliphytes only detected in the 18S rRNA library. MAST (Marine Stramenopiles) appeared as potentially prominent grazers and we observed a significant decrease in the contribution of alveolate and radiolarian sequences, which overwhelmingly dominated rDNA libraries. The rRNA approach appears to be less affected by taxon-specific rDNA copy number and likely better depicts the biogeochemical significance of marine protists Peer reviewed 2013-10-14T12:07:35Z 2013-10-14T12:07:35Z 2009-09 artículo http://purl.org/coar/resource_type/c_6501 PLoS ONE 4(9): e7143 (2009) 1932-6203 http://hdl.handle.net/10261/84047 10.1371/journal.pone.0007143 19787059 en Publisher’s version https://doi.org/10.1371/journal.pone.0007143 open Public Library of Science
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
description 7 pages, 1 figure, 1 table
format artículo
author Not, Fabrice
Campo, Javier del
Balagué, Vanessa
Vargas, Colomban de
Massana, Ramon
spellingShingle Not, Fabrice
Campo, Javier del
Balagué, Vanessa
Vargas, Colomban de
Massana, Ramon
New Insights into the Diversity of Marine Picoeukaryotes
author_facet Not, Fabrice
Campo, Javier del
Balagué, Vanessa
Vargas, Colomban de
Massana, Ramon
author_sort Not, Fabrice
title New Insights into the Diversity of Marine Picoeukaryotes
title_short New Insights into the Diversity of Marine Picoeukaryotes
title_full New Insights into the Diversity of Marine Picoeukaryotes
title_fullStr New Insights into the Diversity of Marine Picoeukaryotes
title_full_unstemmed New Insights into the Diversity of Marine Picoeukaryotes
title_sort new insights into the diversity of marine picoeukaryotes
publisher Public Library of Science
publishDate 2009-09
url http://hdl.handle.net/10261/84047
work_keys_str_mv AT notfabrice newinsightsintothediversityofmarinepicoeukaryotes
AT campojavierdel newinsightsintothediversityofmarinepicoeukaryotes
AT balaguevanessa newinsightsintothediversityofmarinepicoeukaryotes
AT vargascolombande newinsightsintothediversityofmarinepicoeukaryotes
AT massanaramon newinsightsintothediversityofmarinepicoeukaryotes
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