Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton
Cuvelier, Marie L. ... et al.-- 6 pages, 4 figures, 1 table, this article contains supporting information online at https://www.pnas.org/content/pnas/suppl/2010/07/27/1001665107.DCSupplemental/pnas.201001665SI.pdf.-- Data deposition: The sequences reported in this paper have been deposited in the Gen-Bank database (accession nos. HM581528–HM581638 and HM565909–HM565914). Other scaffolds with predicted genes from this Whole Genome Shotgun/454 project have been deposited at DNA Data Bank of Japan/European Molecular Biology Laboratory/GenBank under the accession no. AEAR00000000. The version described in this paper is the first version, AEAR01000000
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| Format: | artículo biblioteca |
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National Academy of Sciences (U.S.)
2010-08-17
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| Subjects: | Comparative genomics, Primary production, Prymnesiophytes, Marine photosynthesis, Haptophytes, |
| Online Access: | http://hdl.handle.net/10261/80830 |
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dig-icm-es-10261-808302021-12-27T15:36:58Z Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton Cuvelier, Marie L. Latasa, Mikel Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes Cuvelier, Marie L. ... et al.-- 6 pages, 4 figures, 1 table, this article contains supporting information online at https://www.pnas.org/content/pnas/suppl/2010/07/27/1001665107.DCSupplemental/pnas.201001665SI.pdf.-- Data deposition: The sequences reported in this paper have been deposited in the Gen-Bank database (accession nos. HM581528–HM581638 and HM565909–HM565914). Other scaffolds with predicted genes from this Whole Genome Shotgun/454 project have been deposited at DNA Data Bank of Japan/European Molecular Biology Laboratory/GenBank under the accession no. AEAR00000000. The version described in this paper is the first version, AEAR01000000 Among eukaryotes, four major phytoplankton lineages are responsible for marine photosynthesis; prymnesiophytes, alveolates, stramenopiles, and prasinophytes. Contributions by individual taxa, however, are not well known, and genomes have been analyzed fromonly the latter two lineages. Tiny >picoplanktonic> members of the prymnesiophyte lineage have long been inferred to be ecologically important but remain poorly characterized. Here, we examine pico-prymnesiophyte evolutionary history and ecology using cultivation-independent methods. 18S rRNA gene analysis showed picoprymnesiophytes belonged to broadly distributed uncultivated taxa. Therefore, we used targeted metagenomics to analyze uncultured pico-prymnesiophytes sorted by flow cytometry from subtropical North Atlantic waters. The data reveal a composite nuclear-encoded gene repertoire with strong green-lineage affiliations, which contrasts with the evolutionary history indicated by the plastid genome. Measured pico-prymnesiophyte growth rates were rapid in this region, resulting in primary production contributions similar to the cyanobacterium Prochlorococcus. On average, pico-prymnesiophytes formed 25% of global picophytoplankton biomass, with differing contributions in five biogeographical provinces spanning tropical to subpolar systems. Elements likely contributing to success include high gene density and genes potentially involved in defense and nutrient uptake. Our findings have implications reaching beyond pico-prymnesiophytes, to the prasinophytes and stramenopiles. For example, prevalence of putative Ni-containing superoxide dismutases (SODs), instead of Fe-containing SODs, seems to be a common adaptation among eukaryotic phytoplankton for reducing Fe quotas in low-Fe modern oceans. Moreover, highly mosaic gene repertoires, although compositionally distinct for each major eukaryotic lineage, now seem to be an underlying facet of successful marine phytoplankton Sequencing was under DE-AC02- 05CH11231, by a Department of Energy Community Sequencing Program award to A.Z.W. and J. Eisen. Support was in part by DE-FC02-02ER63453, NSF OCE-0722374, and NSF-MCB-0732448 (to A.E.A.); a National Human Genomic Research Institute, National Institutes of Health grant (to R.S.L.); National Oceanic and Atmospheric Administration and David and Lucile Packard Foundation (DLPF) grants (F.P.C.); NSF-OCE-0241740 (to B.J.B.); and major funding by NSF-OCE-0836721, the DLPF, and a Moore Foundation Young Investigator Award as well as Moore 1668 (to A.Z.W.). Author contribution details are given in SI Materials and Methods, Section 12 Peer Reviewed 2013-08-22T06:47:52Z 2013-08-22T06:47:52Z 2010-08-17 2013-08-22T06:47:52Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1073/pnas.1001665107 issn: 0027-8424 e-issn: 1091-6490 Proceedings of the National Academy of Sciences 107: 14679-14684 (2010) http://hdl.handle.net/10261/80830 10.1073/pnas.1001665107 20668244 en https://doi.org/10.1073/pnas.1001665107 none National Academy of Sciences (U.S.) |
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Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes |
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Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes Cuvelier, Marie L. Latasa, Mikel Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
| description |
Cuvelier, Marie L. ... et al.-- 6 pages, 4 figures, 1 table, this article contains supporting information online at https://www.pnas.org/content/pnas/suppl/2010/07/27/1001665107.DCSupplemental/pnas.201001665SI.pdf.-- Data deposition: The sequences reported in this paper have been deposited in the Gen-Bank database (accession nos. HM581528–HM581638 and HM565909–HM565914). Other scaffolds with predicted genes from this Whole Genome Shotgun/454 project have been deposited at DNA Data Bank of Japan/European Molecular Biology Laboratory/GenBank under the accession no. AEAR00000000. The version described in this paper is the first version, AEAR01000000 |
| format |
artículo |
| topic_facet |
Comparative genomics Primary production Prymnesiophytes Marine photosynthesis Haptophytes |
| author |
Cuvelier, Marie L. Latasa, Mikel |
| author_facet |
Cuvelier, Marie L. Latasa, Mikel |
| author_sort |
Cuvelier, Marie L. |
| title |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
| title_short |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
| title_full |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
| title_fullStr |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
| title_full_unstemmed |
Targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
| title_sort |
targeted metagenomics and ecology of globally important uncultured eukaryotic phytoplankton |
| publisher |
National Academy of Sciences (U.S.) |
| publishDate |
2010-08-17 |
| url |
http://hdl.handle.net/10261/80830 |
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AT cuveliermariel targetedmetagenomicsandecologyofgloballyimportantunculturedeukaryoticphytoplankton AT latasamikel targetedmetagenomicsandecologyofgloballyimportantunculturedeukaryoticphytoplankton |
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