Exploring Why Dinoflagellates Are So Sensitive To Small-Scale Turbulence.The Microtubule Assemblage Alteration Hypothesis
14th International Conference on Harmful Algae, 1-5 November 2010, Creta, Greece
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Hellenic Centre for Marine Research
2010-11
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Online Access: | http://hdl.handle.net/10261/80493 |
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dig-icm-es-10261-804932019-03-06T11:50:52Z Exploring Why Dinoflagellates Are So Sensitive To Small-Scale Turbulence.The Microtubule Assemblage Alteration Hypothesis Berdalet, Elisa Llaveria, Gisela Huitorel Philippe 14th International Conference on Harmful Algae, 1-5 November 2010, Creta, Greece Small-scale turbulence has been shown to slow down the growth and/or some phases of the cell cycle of some dinoflagellate species, and it seems that turbulence or shear stress could alter the cell division processes. Here, we report our studies to investigate the unknown underlying mechanisms of the observed responses. We tested the hypothesis that turbulence could alter the correct assemblage of microtubules composing the mitotic spindle involved in the chromosome segregation during the dinomitosis. In shaking experiments performed on Akashiwo sanguinea, Prorocentrum micans and Oxyrrhis marina, microtubules were visualized through a combination of inmunocytochemistry (using anti-tubulin monoclonal antibodies) Confocal Fluorescent Laser Microscopy. Each species showed particular organizations of the nuclear, microtubular and other mitotic elements under still conditions. These mitotic organizations appeared unmodified by turbulence. Thus, our results did not provide evidences to support the microtubule hypothesis. Some alternative hypotheses also linked to the microtubular organization in the cell are suggested Peer Reviewed 2013-08-05T06:29:51Z 2013-08-05T06:29:51Z 2010-11 2013-08-05T06:29:52Z comunicación de congreso http://purl.org/coar/resource_type/c_5794 14th International Conference on Harmful Algae. Abstract book: 187 (2010) http://hdl.handle.net/10261/80493 en http://www.issha.org/Welcome-to-ISSHA/Conferences/ICHA-Conferences/Past-conferences none Hellenic Centre for Marine Research |
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14th International Conference on Harmful Algae, 1-5 November 2010, Creta, Greece |
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comunicación de congreso |
author |
Berdalet, Elisa Llaveria, Gisela Huitorel Philippe |
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Berdalet, Elisa Llaveria, Gisela Huitorel Philippe Exploring Why Dinoflagellates Are So Sensitive To Small-Scale Turbulence.The Microtubule Assemblage Alteration Hypothesis |
author_facet |
Berdalet, Elisa Llaveria, Gisela Huitorel Philippe |
author_sort |
Berdalet, Elisa |
title |
Exploring Why Dinoflagellates Are So Sensitive To Small-Scale Turbulence.The Microtubule Assemblage Alteration Hypothesis |
title_short |
Exploring Why Dinoflagellates Are So Sensitive To Small-Scale Turbulence.The Microtubule Assemblage Alteration Hypothesis |
title_full |
Exploring Why Dinoflagellates Are So Sensitive To Small-Scale Turbulence.The Microtubule Assemblage Alteration Hypothesis |
title_fullStr |
Exploring Why Dinoflagellates Are So Sensitive To Small-Scale Turbulence.The Microtubule Assemblage Alteration Hypothesis |
title_full_unstemmed |
Exploring Why Dinoflagellates Are So Sensitive To Small-Scale Turbulence.The Microtubule Assemblage Alteration Hypothesis |
title_sort |
exploring why dinoflagellates are so sensitive to small-scale turbulence.the microtubule assemblage alteration hypothesis |
publisher |
Hellenic Centre for Marine Research |
publishDate |
2010-11 |
url |
http://hdl.handle.net/10261/80493 |
work_keys_str_mv |
AT berdaletelisa exploringwhydinoflagellatesaresosensitivetosmallscaleturbulencethemicrotubuleassemblagealterationhypothesis AT llaveriagisela exploringwhydinoflagellatesaresosensitivetosmallscaleturbulencethemicrotubuleassemblagealterationhypothesis AT huitorelphilippe exploringwhydinoflagellatesaresosensitivetosmallscaleturbulencethemicrotubuleassemblagealterationhypothesis |
_version_ |
1777665939639107584 |